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Biomedical subjects

E Righi

Publications and source records attributed to E Righi.

At least 37 records · Page 2Linked to original sources

Experimental analysis of internal rigid fixation osteosynthesis performed with titanium bone screw and plate systems.

The authors report the results of an experimental analysis performed on titanium miniplates and screws in order to gain a better understanding of dynamic forces in internal rigid fixation. Ten segments of bovine scapula were prepared. Osteotomies were carried out along the minor axis, following which five were fixed with four hole straight miniplates and the other five with six hole double-Y miniplates. Each sample was fastened in a special clamp adapted to a tension test machine and shearing force was applied. Force versus time was recorded and the 50 bone fragments were examined by a pathologist. On the basis of the test results, two simple computer models were developed. No significant difference was evident between the mechanical and computed tests. The most critical sections were located near the hole proximal to the osteotomy and the microscopic findings confirmed this. On the basis of the experimental results, the authors propose a new plate design in which the area subject to most stress, proximal to the bone section, would be of miniplate thickness, the distal aspect being thinner as in a microplate. It is suggested that this design would provide sufficient stability and a high degree of anatomical adjustment of the system.

Animals↗

Micronuclei in cytokinesis-blocked lymphocytes may predict patient response to radiotherapy.

We have applied the cytokinesis-block micronucleus assay to peripheral blood lymphocytes of patients undergoing radiotherapy in pelvic and pulmonary sites, in order to evaluate the individual cytogenetic response. Our cytogenetic data correlated with the equivalent whole-body dose are homogeneous and compare well with the data presented by other authors. We have used an exponential mathematical formula to calculate the attenuation of the cytogenetic effect with time. The k coefficient (cytogenetic recovery factor) in the formula expresses the degree of attenuation. In lymphocytes from patients after radiotherapy, the trend of the micronucleus frequency observed after 2 Gy of in vitro X-irradiation demonstrates that the cytogenetic effect obtained in vitro is added to that obtained in vivo. The k coefficient is inversely proportional to the micronucleus frequency observed after 2 Gy in vitro. The micronucleus assay and the cytogenetic recovery factor are proposed as suitable diagnostic tools for application in the field of radiotherapy.

Dose-Response Relationship, Radiation↗

Environmental and biological monitoring of chloroform in indoor swimming pools.

The presence of chloroform as the result of disinfection with sodium hypochlorite was demonstrated in the water and ambient air of indoor swimming pools. Environmental monitoring was performed in 12 indoor swimming pools in northern Italy and the level of human exposure was assessed. Biological monitoring performed by gas chromatography on human plasma and alveolar air samples evidenced that the uptake of chloroform in swimmers varies according to the intensity of the physical activity and age. The elimination of chloroform in alveolar air in one subject showed a very short half-life (from 20 to 27 min) and a complete clearance within 10 h after the end of exposure.

Breath Tests↗

Micronuclei and 3AB index in X-irradiated human lymphocytes in G0 and G1 phases.

We applied the cytokines-block micronucleus assay to observe the radiobiological response of human lymphocytes after X-ray treatment in the G0 and G1 phases. In addition, we used 3-aminobenzamide (3AB) to measure the 3AB index in the two phases. The experimental results show that at 2 Gy the MN yield and the 3AB index are dependent on the cell phase and show considerable inter-individual variability. The radiation-induced MN frequency obtained for 33 subjects is 0.470 +/- 0.063 for the G0 phase and 0.689 +/- 0.139 for the G1 phase; the 3AB index values are 0.326 +/- 0.144 and 0.067 +/- 0.058 for G0 and G1 phases, respectively. At the individual level, the 3AB index for the G1 phase correlates inversely with the cytogenetic effects observed in that phase. We discuss the possibility of applying the MN test combined with the 3AB index to lymphocytes at different phases to study the individual response to radiation (individual radiosensitivity).

Adult↗

Indoor exposure to perchloroethylene (PCE) in individuals living with dry-cleaning workers.

Perchloroethylene (PCE) is the most widely used solvent in dry-cleaning; it is toxic to the liver, kidney and central nervous system and may be a human carcinogen. PCE levels in the ambient air of dry-cleaners' homes were measured, and samples of end-exhaled air (alveolar air) from subjects who were not themselves occupationally exposed, but who were members of the household of dry-cleaners were compared with samples from the general population. Thirty apartments were visited housing dry-cleaners and their families, and located well away from the dry-cleaning premises. Indoor air samples and alveolar air samples were collected contemporaneously from the dry-cleaners (36) and members of their household (34). The same sampling procedure was followed in 25 private homes where samples of alveolar air were collected from 41 subjects who were not occupationally exposed and who acted as control group. All the samples were analysed by direct-injection gas-chromatography. PCE levels in dry-cleaners' homes proved to be significantly higher than in control houses (geometric means: 265 vs. 2 micrograms/m3, P < 0.001). PCE levels in the alveolar air exhaled by dry-cleaners, their family members and control subjects were statistically different (geometric means: 5140, 225 and 3 micrograms/m3, respectively; P < 0.001). PCE is a ubiquitous substance in indoor air, but is present at higher concentrations in apartments where dry-cleaners and their families live. Biological monitoring of PCE in alveolar air confirms that family members of dry-cleaners are more exposed than the general population.

Adult↗

Perchloroethylene exposure can induce colour vision loss.

We evaluated colour vision in 35 dry-cleaners exposed to perchloroethylene (PCE) and in a paired number of controls matched for sex, age, alcohol consumption and cigarette smoking. A subclinical colour vision loss, mainly in the blue-yellow range, was present in dry-cleaners. This effect was related to PCE exposure levels, and appeared at environmental concentrations of the solvent well below the current exposure limits for exposed workers. The results suggest that PCE exposure, even at low environmental levels, can induce a dose-related impairment of colour vision.

Adult↗

Micronuclei and 3AB index in human and canine lymphocytes after in vitro X-irradiation.

The comparative study of different species could be of interest, both applied and pure, to the field of cytogenetic damage induced by genotoxic agents. For as accurate as possible an evaluation of the inter-species response differences to radiation, we have carried out a comparison between the behaviors of human and canine lymphocytes, using the micronucleus assay (MN test) according to the cytokinesis-block method. Up to 4 Gy doses, canine lymphocytes have been found to be about three times more radiosensitive than human lymphocytes, due to blastization inhibition (binucleation failure), and, for 1 and 2 Gy doses, about 1.3 times more radiosensitive, due to MN yields. We discuss whether the differing chromosome number (dog 78 and man 46) could have any effect on the cytogenetic response. 3-Aminobenzamide, which inhibits poly(ADP-ribose)polymerase activity, is able to increase the genotoxic effect of X-rays in human lymphocytes, with a different response at the individual level. The same phenomenon with the same characteristics is also found in canine lymphocytes at the inter-individual level. Our in vitro radiobiological study confirms that the cytogenetic response obtained in blood from selected breeds of mammalian species can be utilized for applications in environmental studies.

Animals↗

Peters'-Plus syndrome: report on an unusual case.

We report on a 10 1/2-years-old patient with clinical manifestations of Peters'-Plus syndrome. The patient had Peters' anomaly, slightly arched eyebrows, long eyelashes, a broad nasal tip; malformed and prominent ears, cleft lip and palate, brachydactyly, fingertip pads, severe growth deficiency, and mild mental retardation. Peters' anomaly is a defect of the anterior chamber of the eye, including central corneal opacity, thinning of the posterior aspect of the cornea and iridolenticulocorneal adhesions. Peters'-Plus syndrome is a disorder in which patients with Peters' anomaly are also found to have short stature, brachymedia, abnormal ears, cleft lip and palate, and mental retardation. Our patient showed Peters'-Plus syndrome associated with some clinical manifestations of the Kabuki make-up syndrome.

Abnormalities, Multiple↗

Micronuclei and 3AB-index in patients receiving iodine-131 therapy.

In 28 blood samples from 21 patients undergoing 131I treatment after surgical thyroidectomy for cancer, the micronucleus (MN) frequency observed in peripheral blood lymphocytes (MN-test on binucleated cells) had a weighted mean of 0.044 +/- 0.006 (SEM), which was significantly different (p < 0.001) from that observed in 93 healthy individuals (0.025 +/- 0.001). The MN frequency (F(MN)) of the patients correlated fairly well (R = 0.736) with the modified activity (Amod) calculated by the following equation: [formula: see text] where Ai is the 131I activity on a determined day, e the logarithm base, di the number of days that have passed between the determined day and the day when the blood was drawn, and k is a day coefficient, defined in this context as the "daily attenuation factor". The use of the value of 0.0003 for k allowed the following equation to be obtained: F(MN) = 7.9 x 10(-5) (+/- 1.4 x 10(-5)).Amod + 0.014 (R = 0.736) The MN frequency was used to estimate, by our DOSIME program, the dose (Gy) received at the individual level in the 131I treatment. In these subjects the calculated dose was well correlated with Amod by the relationship: DBio = 0.0009 (+/- 0.0002).Amod + 0.0675 (R = 0.755) 3-aminobenzamide (3AB), an inhibitor of the poly(ADP-ribose)polymerase enzyme involved in DNA repair, induced and increase in X-ray cytogenetic damage (MN yields), evaluated at the individual level using the 3AB-index (I-3AB). The index was obtained from the MN-yield count after x-irradiation with (MN + 3AB) and without (MN - 3AB) 3AB, using the following formula: I = 1-(MN - 3AB/MN + 3AB).(ABSTRACT TRUNCATED AT 250 WORDS)

Humans↗

Toriello-Carey syndrome: report of a new case.

Toriello-Carey syndrome is a malformation syndrome characterized by cranio-facial defects, visceral defects, congenital heart defects, hypotonia, and postnatal growth retardation. To our knowledge, only five patients with this condition have been reported. We describe another case of the Toriello-Carey syndrome extending the phenotypic manifestations of the syndrome.

Abnormalities, Multiple↗

Inter-individual differences in radiation response shown by an in vitro micronucleus assay: effects of 3-aminobenzamide on X-ray treatment.

Among the methods of biological dosimetry of ionizing radiation, we propose the cytokinesis-block micronucleus assay for the measurement of the individual dose absorbed. The dose-response curve was determined for in vitro-irradiated lymphocytes from 25 individuals. The dose-response relationship, fitted by the linear-quadratic function, was F(MN) = 0.015 (+/- 0.0016) + 0.043 (+/- 0.0075).D + 0.083 (+/- 0.0045).D2. Our results are compared with those of other authors. 3-aminobenzamide (3AB) combined with X-rays were used to evaluate the micronucleus dose-response relationship in blood from 14 individuals. While it is known that 3AB inhibits poly(ADP-ribose) polymerase activity in vitro, we demonstrate that it also increases the X-ray-induced micronucleus yields. The resulting dose-response relationship varies from subject to subject. The possibility of using this approach to identify the individual radiosensitivity level is discussed.

Adult↗

Reference values for some fundamental haemostatic factors in the dog.

The study is a methodological survey to define the reference values for some basic haemostatic parameters in healthy dogs. Reference values for PT, APTT and factors VIII:C and IX:C were studied in 34 dogs. The reference values were (minimum-maximum) 6.5-9.5 and 17.5-26.3 seconds for PT and APTT, 58-260% and 67-163% for VIII:C and IX:C respectively and 185.8-354.4 x 10/1 for platelets. These reference values were applied to 130 other dogs which were presented to the clinic for various treatments or health checks. The results show how haemostatic analysis can be used to resolve delicate interpretation of laboratory results.

Animals↗

[Aspects of industrial hygiene in occupational exposure to the hazards of laser light].

The AA. outline the environmental hazards by the laser light taking into account the characteristics of this stimulated emission and the properties of the biological substrate to be damaged. They illustrate the optical and functional behaviour of the eye in order to demonstrate the peculiarities which make it the most important "critical organ" as regards the laser light. They furthermore propose some criteria for the analysis and the registration of the data necessary to individuate the hazards. They indicate the industrial hygiene measures to be adopted in the different exposure conditions and point out some aspects apt to individuate the levels for minimal reactive dose from which it is possible to define the threshold limit values. They conclude with the proposal to normalize as much as possible the rules of hygienistic protection in this new technological field.

Environmental Exposure↗