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Biomedical subjects

E Razin

Publications and source records attributed to E Razin.

63 records · Page 4Linked to original sources
PubMed1981

Growth of a pure population of mouse mast cells in vitro with conditioned medium derived from concanavalin A-stimulated splenocytes.

A pure population of mast cells was obtained after 14 days of culturing mouse bone marrow cells in the presence of medium derived from concanavalin A-stimulated mouse spleen cells. The cells were characterized as mast cells by their morphologic appearance and histologic staining, by their histamine content (450 ng per 10(6) cells) and by the demonstration of IgE receptors on their surface (150,000--440,000 receptor sites per cell). The histamine content and the number of IgE receptors remained constant for at least 7 wk of culture. These mast cells could be passively sensitized to mice hybridoma IgE. They then released 43% of their histamine content upon incubation with anti-mouse hybridoma IgE.

Animals↗
PubMed1981

Selective growth of a population of human basophil cells in vitro.

An initially homogeneous population of basophilic polymorphonuclear leukocytes was derived from human fetal liver cells grown in culture for 5-7 days. The cells were characterized as basophils by their morphology, histologic staining characteristics, and histamine content, and by the presence of IgE receptors on their surface. In this culture system the basophils were viable for up to 10 days.

Basophils↗
PubMed1979

Function of macrophage prostaglandins in the process of phagocytosis.

The role of prostaglandins (PGs) in phagocytosis of sheep erythrocytes (SRC) by mouse spleen adherent cells was examined. A decrease of about 50% in the phagocytosis was observed when macrophages were treated with 5 microgram/ml indomethacin. Concomitant addition of PGs (10(-6) mg/ml), however, prevented the inhibitory effect of indomethacin. It is thus suggested that PGs play an important role in phagocytosis.

Animals↗
PubMedDate not supplied

Studies of different aspects of the role of protein kinase C in mast cells.

Mast cells induce the inflammatory process when their FcepsilonRI receptors aggregate in response to an antigen binding to immunoglobulin E. Direct interactions between FcepsilonRI receptor cytoplasmic domains and various intracellular proteins initiate diverse signal transduction pathways resulting in the immediate release of proinflammatory agents. A delayed response also occurs that includes the release of various cytokines. It is clear that the activation of kinases, such as protein kinase C (PKC), is a requirement for both the early and delayed responses of this inflammatory process. In this review we present the results of various studies investigating the role of PKC isozymes in mast cells.

Animals↗
PubMedDate not supplied

The incapability of interleukin-4 to induce AP-1 activity in murine mast cells.

The results presented in this study shed new light on the molecular mechanism responsible for the control of interleukin (IL)-3- and IL-4 mediated mast cell proliferation. By measurements of AP-1 DNA-binding activity, it was found that IL-3 induced such activity while IL-4 did not. This difference in the pattern of AP-1 DNA-binding activity induced by each lymphokine indicates the differential involvement of AP-1 in the different proliferative responses of mast cells to IL-3 and IL-4.

Alkaloids↗