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Biomedical subjects

E R Stout

Publications and source records attributed to E R Stout.

27 records · Page 2Linked to original sources
PubMed1978

Levels of cellular DNA polymerases in synchronized bovine paravovirus-infected cells.

Infection of synchronized bovine fetal spleen cells with bovine parvovirus results in changes in the levels and patterns of DNA polymerases alpha and gamma during the cell cycle. The pattern of DNA polymerase alpha activity closely paralled viral DNA synthesis and the production of progeny virus, and levels, of this enzyme were threefold greater than in mock-infected cells during the period of maximal viral DNA synthesis. DNA polymerase gamma activity remained slightly elevated during viral DNA replication. Levels and patterns of DNA polymerase beta were similar in mock- and virus-infected cells.

Animals↗
PubMed1968

Template requirement of maize RNA polymerase.

Maize RNA polymerase utilizes heated deoxyribonucleic acid more effectively than native deoxyribonucleic acid as a template for ribonucleic acid synthesis. A ribonucleic acid-deoxyribonucleic acid hybrid accumulates in the presence of heated deoxyribonucleic acid. The amount of product formed with either native or heat-denatured deoxyribonucleic acid does not exceed the amount of deoxyribonucleic acid added as template.

Journal Article↗
PubMed1966

Role of oxygen fixation in hydroxyproline biosynthesis by etiolated seedlings.

Etiolated maize and soybean seedlings were grown for several days in atmospheres enriched with O(18). Hydroxyproline subsequently isolated from the seedlings by column and thin-layer chromatography was labeled with excess O(18), but proline was not. Control experiments in which seedlings were grown in H(2)O(18) and unlabeled atmospheres demonstrated that neither proline nor hydroxyproline was labeled with excess O(18). It was concluded that oxygen fixation is an essential feature of hydroxyproline biosynthesis in these seedlings, and that the hydroxyl oxygen atom in hydroxyproline is derived from molecular oxygen and not from water; similar results have been reported previously for sycamore cell suspensions.

Journal Article↗