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Biomedical subjects

E Quirós

Publications and source records attributed to E Quirós.

28 records · Page 2Linked to original sources
PubMed1993

[Evaluation of DNA-HIV detection in various population groups].

BACKGROUND: To evaluate the diagnostic use of Polymerase Chain Reaction (PCR) for the detection of HIV-DNA. METHODS: We have investigated HIV-DNA in peripheral blood mononuclear cells of 150 individuals: 50 seronegative without recognized factors to acquire HIV infection, 50 seronegative that showed risk habits and 50 seropositive. For amplification we have used primers SK38/SK39 which amplify a conserved region of 114 bp from HIV's gag gen. The detection of the amplificate was performed using solution hybridization with 32P-labelled SK 19, 6% polyacrylamide gel electrophoresis and autoradiography. RESULTS: We have detected HIV-DNA at every seropositive patient (100%), 6 samples from seronegative individuals that showed risk habits and 1 sample from non-risk seronegative individuals. CONCLUSIONS: Polymerase chain reaction represents a diagnostic tool at HIV infection, specially for those patients where seroconversion can not be demonstrated by conventional methods.

DNA, Viral↗
PubMed1992

[Annual cost of microbial immunological tests for HIV-positive individuals in a hospital center].

The goal of this study is to increase the knowledge of the AIDS cost in a hospital. We have studied the HIV patients diagnosed during a year in our hospital and the charges of the serological tests performed to them. 20.8% of the tests were to study antibodies against HIV (only 20% were positive). After patient distribution between extra or intrahospital origin, we highlight that 61% of the charges are attributed to patients who do not belong to the hospital but to its catchment area. We emphasize the problem that AIDS patients are creating to hospital, being necessary that personal select only useful tests honestly.

AIDS Serodiagnosis↗
PubMed1992

[Quantification of the levels of p24 antigen and antibodies in human immunodeficiency virus infection].

BACKGROUND: Quantification and clinical evaluation of p24 antigen and anti-HIV antibody levels. METHODS: Follow up of 13 HIV infected patients (53 sera) by determination of p24 antigen, total anti-HIV antibodies, anti-p24 and anti-env antibodies by ELISA and their semiquantitation. IgG and IgM class antibody determination by immunoblot techniques. RESULTS: The highest levels of p24 have been found in WR 1 and WR 6 stages. Commonly accepted serologic pattern has not been found in 100% of our cases. Western-blot is more sensitive for the detection of anti-p24 antibodies. CONCLUSIONS: p24 antigen appearance and a decrease in anti-p24 antibody level is related to a worse clinical prognosis. p24 antigen quantification is a usefull test for monitoring AZT therapy in patients suffering from AIDS.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Immune complex p24 antigen: a new prognostic marker in human immunodeficiency virus infection.

p24 antigen (p24 Ag) is usually not found in patients in the asymptomatic phase of HIV infection, mainly because it is bound to anti-p24 antibodies (p24 Ab), with which it forms immune complexes. Nine asymptomatic patients positive for HIV-1 antibodies (stage II CDC criteria) treated with r alpha-interferon were followed (7.5 +/- 0.5 months), and a basal and final sample were tested for p24 Ag immune complexes (p24 Ag-IC), free p24 Ag, p24 Ab, CD4 count, beta 2-microglobulin, properdin B factor, and C3 and C4 complement fractions. p24 Ag-IC was detected in 55.5% of the samples, whereas free p24 Ag was detected in 5.5%. The finding of no change or an increase in p24 Ag-IC serum levels may be considered a prognostic marker. We believe that p24 Ab cannot be used as a prognostic marker unless p24 Ag-IC detection is simultaneously evaluated.

Antigen-Antibody Complex↗
PubMedDate not supplied

[Liver cirrhosis: pulmonary function].

We performed a functional respiratory examination which consisted of arterial gasometry, spirometry, diffusion capacity to CO2, alveolo-arterial gradient of O2 and pulmonary volumes to 8 patients with cirrhosis diagnosed by clinical history, laboratory exams, abdominal ultrasound and histology. Our results showed a slight obstructive pattern of peripheric airways (FMM: 88.87 +/- 8.7%) in the spirometry, no difference in arterial gases at upright and recumbent position was observed, with low values of apO2 (75.51 +/- 1.16 upright and 75.87 +/- 2.16 mmHg recumbent) without statistic significance. The gradient G(Aa) O2 increased to (30.89 +/- 1.06 mmHg). Besides there was a diffusion abnormality with a DLCO2/VA of (71.87 +/- 6.05%). Breathing 100% O2, did not change the gradient which allows us to postulate the existence of an abnormality of gaseous interchange due to shunts. We found no relationship between albumin levels and DLCO2/VO neither with pO2 in upright position; there was a relationship at recumbent position between the hepatic disorder and the arterial desaturation. We concluded that there is no significant hypoxia even with position changes, there is increase of G (Aa) O2 by shunt type disorders and that this is probably related with albumin levels.

Adult↗
PubMedDate not supplied

[Clinical and prognostic factors of mortality in upper digestive hemorrhage].

In order to investigate prognostic factors we prospectively followed 741 patients with upper gastrointestinal bleeding (UGIB). Eleven variables were identified with mortality predictive value. Hematemesis, age of 50 y/o, more than 120 heart beat/minute, 3 or more units of pack red blood cells, esophageal varices, cirrhotic liver and renal failure had independent predictive value. Our findings could help to identify high risk patients.

Age Factors↗