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Biomedical subjects

E Piasecki

Publications and source records attributed to E Piasecki.

31 records · Page 2Linked to original sources
PubMed1989

Psychological support and psychiatric management of patients with automatic implantable cardioverter defibrillators.

There are well over 6,000 automatic implantable cardioverter defibrillators (AICD) that have been implanted in the United States since 1980. The device clearly reduces arrhythmic mortality in high risk patients. Many AICD patients have other cardiac diseases, most commonly, coronary artery disease with associated prior myocardial infarctions. These patients have special psychosocial stresses that may make them vulnerable to depression and other psychological disturbances. Psychological support together with psychiatric treatment, if needed, should be provided to these patients. Use of psychotropic medications should be closely monitored, especially in this population, as many of these drugs can exacerbate ventricular tachyarrhythmias. Special precautions should also be taken when administering electroconvulsive therapy in these patients.

Adjustment Disorders↗
PubMed1988

Properties of natural porcine interferons.

Natural porcine interferons (PoIFNs) were obtained from: adherent peripheral blood leukocytes induced with Newcastle disease virus (NDV) (IFN-alpha), kidney fibroblasts induced with poly(I:C) (IFN-beta) and whole blood leukocytes induced with phytohemagglutinin (IFN-gamma). PoIFN-alpha was active in porcine, bovine, human, and murine cells. It was sensitive to anti-human IFN-alpha antiserum. Analyzed by SDS-polyacrylamide gel electrophoresis and by gel filtration, the molecular weight of the main fraction of PoIFN-alpha was between 25,200 and 28,700. PoIFN-beta and a small fraction of PoIFN-alpha were bound to concanavalin A which suggests that they are glycoproteins. PoIFN-beta and PoIFN-gamma were active only in porcine and bovine, and porcine cells, respectively. Physicochemical properties of porcine IFNs resembled those of human IFNs. PoIFN-alpha or PoIFN-beta and PoIFN-gamma acted synergistically in the antiviral assays. Thus, the porcine IFN system appears to be similar to the system in the human and in murine species, being serologically related to the former.

Animals↗
PubMed1988

1,4-naphthoquinone-2-sulfonic acid derivatives coupled to dextran as carriers for interferon.

Three derivatives of 1,4-naphthoquinone-2-sulfonic acid possessing the fragments of Cibacron Blue (CB) were synthesized and bound to Dextran T 2000 by ether binding. Polymers IVD, VD and VID were incubated with interferon (IFN) to obtain complexes: carrier-IFN. It was found that polymer VID has weaker affinity to mouse IFN-beta/alpha and to human IFN-beta than Blue Dextran. The polymers IVD and VD had no affinity to the interferon.

Affinity Labels↗
PubMed1988

Effects of interferons, interferon inducers and growth factors on phagocytosis measured by quantitative determination of synthetic compound ingested by mouse bone marrow-derived macrophages.

Sodium salt of 9-oxo-10-acridineacetohydroxamic acid (HCA), a new synthetic compound, forms small crystals in aqueous solution. These crystals were easily phagocytized by the mouse bone marrow-derived macrophages. The ingested HCA crystals were visible under light microscope as dark granules. The degree of phagocytosis was estimated by the spectroscopic measurements of absorption of ingested HCA. About 10 day-old cultures of mouse bone marrow-derived macrophages were found to be suitable for a study on the effect of murine or human interferons. It was observed that murine interferons alpha, beta and gamma at low concentrations (10-200 U/ml) stimulated and higher concentrations (400-1000 U/ml) had no effect on the phagocytosis. Previous treatment of interferons with anti-IFN sera abolished the effect of the interferons. CMA-induced interferon and growth factors were found to modify the phagocytic activity of macrophage cultures.

Acridines↗
PubMed1988

Assessment of interferon production in patients with chronic nonviral diseases by whole blood or whole bone marrow techniques.

Hundred and fifty-four samples of heparinized blood from patients with chronic toxoplasmosis, chronic brucellosis, lung diseases, psychiatric affective disorders, healthy individuals and neonates, were obtained. The experimental material included also 14 bone marrow samples from patients with hematological diseases or lung cancer. The whole cell populations were treated with several classical IFN inducers and supernatants were assayed for IFN activity in human lung adenocarcinoma cell line A 549 using VSV or EMC viruses as challenge. The response of WBC to NDV or PHA + PMA was high and remarkably stable in majority of cases except listed below. The levels of IFN induced by LPS were low. The response of WBMC to IFN inducers closely resembled the response of WBC. Low IFN levels in samples of stimulated WBMC from some hematological patients were connected with low cellularity. WBC from psychiatric patients with affective disorders weakly responded to stimulation with NDV. This suggested an existence of deficiency of IFN alpha production connected with affective disorders. Also WBC from cord blood failed to react to PHA + PMA and were poorly responsive to NDV.

Adolescent↗
PubMed1988

A simple colorimetric method for the measurement of phagocytosis of crystals of low molecular weight compound by macrophages.

Sodium salt of 9-oxo-10-acridineacetohydroxamic acid (HCA), a new synthetic compound, forms small crystals in aqueous solutions. These crystals were easily phagocytable by the mouse bone marrow-derived macrophages. The ingested HCA crystals were visible under light microscope as dark granules. The counting of cells with the granules allowed the calculation of number of the phagocytic cells in the preparation. It was also possible to measure the amount of ingested HCA by the cells using colorimetric method. The kinetics of phagocytosis of HCA showed that the process was rapid. Quantitative measurements of ingestion of HCA reflected the phagocytic activity of the cultured cells.

Acridines↗
PubMed1987

New benzoquinone derivatives of dextran as carriers for interferon.

A series of derivatives of benzoquinone-2-sulfonic acid (I-VI) containing constituents of the structure of Cibacron Blue (CB) was synthesized and bound to dextran T 2000 with ether binding (VII-XII). Two dextran polymers XI and XII showed in biological assays high affinity for interferon (IFN).

Binding Sites↗
PubMed1987

Synergistic action of interferons (IFN-alpha/beta and IFN-gamma) of different species origin.

The effects of the mixtures of human, porcine and murine interferons (IFNs) alpha, beta and gamma and bovine IFN-alpha were studied in human, porcine and murine cells. The synergistic action of the mixtures of IFN-alpha/beta and IFN-gamma occurred not only in the homologous but also in the heterologous systems. The synergistic effect was observed when one of the IFNs was of alpha/beta type and the other one of gamma type and when both of them were active in the target cells, regardless of their species origin. Because PoIFN-alpha is active in the cells of numerous mammalian species, it is a good tool for the studies on the synergistic effects. The synergistic action of the mixture of IFNs was abolished after inactivation of one of the IFNs. Specific inactivation of IFN-alpha/beta in the mixture was accomplished by incubation with anti-IFN sera and in the case of IFN-gamma, by acidifying to pH 2.

Animals↗
PubMed1986

A few distinct 'molecular sandwiches' are basis for structural and functional similarities of subspecies of interferon alpha and of families of growth-promoting hormones.

'Molecular sandwiches' composed of two aromatic amino acids separated by a hydrophilic one were found on eleven subspecies of human interferon alpha, on murine interferon alpha 2, and human interferon beta 1. In addition, another type of the sandwiches was found on several species of interferon. This confirms and extends the observations concerning the similarities between some interferons and several classical hormones. Furthermore, we are presenting evidence that a distinct type of the 'molecular sandwiches': Tyr-Cys...Cys and/or Cys...Cys-Cys...Cys, that participate in formation of disulfide bonds, is a characteristic marker of most, if not all of the growth-promoting hormones including growth factors. The 'sandwiches' appear to be important for receptor binding.

Amino Acid Sequence↗
PubMed1985

Interaction of sodium salt of 9-oxo-10-acridineacetic acid (CMA) and its analogs with serum albumin. A model for study on binding of the interferon inducer with receptor.

Equilibrium dialysis, gel filtration and SDS polyacrylamide gel electrophoresis were used to study the interaction of sodium salt of 9-oxo-10-acridineacetic acid (CMA) as well as its analogs 7, 8, 11, 13 - 16 with proteins. The compounds were found to bind mainly to serum albumins. Several other proteins had no affinity to the compounds. The close analogs 7 and 8 (sodium salt of 2,7-dibromo-9-oxo-10-acridineacetic acid and sodium salt of 9-oxo-10-acridinebutyric acid) which were inactive as interferon inducers were found to have greater affinity to bovine, mouse or human albumin than the active IFN inducer--CMA. The mechanism of interaction of CMA as well as its close analogs with albumin resembled the first phase of reaction of pharmacologically active ligands with their specific receptor or acceptor proteins. CMA and some of its close analogs were also shown to stabilize the human erythrocyte membrane against hemolysis in the hypotonic solution. However, the activity of the compounds was much weaker than that of other so called membrane active drugs.

Acridines↗
PubMed1984

New derivatives of blue dextran binding and stabilizing human or mouse interferons.

Mouse or human interferons were found to bind strongly to Blue Dextran 2000 and its eight new derivatives (compound VII to XIV covalently bound to Dextran 2000). The congeners of BD were pale violet or grey. The affinity of Blue Dextran and its congeners to interferons were: IFN-beta greater than IFN-alpha greater than IFN-gamma. The carriers stabilized the antiviral activity of beta-type of interferon during storage at 4 degrees C.

Animals↗