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Biomedical subjects

E Paluska

Publications and source records attributed to E Paluska.

At least 37 records · Page 2Linked to original sources
PubMed1981

Partial characterization of a murine lymph node activating factor released in cultures of H-2 incompatible lymphocytes.

By fractionation on Sephadex columns of the supernatants from mixed cultures of H-2 incompatible lymphocytes we succeeded in partially purifying a lymph node activating factor and in determining its molecular weight. The properties of the lymph node activating factor correspond to those of substances with 16 000 +/- 5 000 D, 65 000 +/- 10 000 D, and also substances with molecular weight higher than 200 000 D which we consider to be aggregates of the minor components. The lymph node activating factor resembles in its molecular weight and effects (in vitro lymph node activation) the products of activated lymphocytes described by other investigators.

Animals↗
PubMed1980

Immunosuppressive effects of a synthetic polymer poly N-(2-hydroxypropyl)methacrylamide (Duxon).

Poly N-(2-hydroxypropyl)methacrylamide (Duxon) was studied as a substitute of blood plasma and was found to prolong significantly the survival of semiallogeneic skin grafts in mice and rats and to reduce MLC reactions of human lymphocytes. It did not inhibit the growth of lymphocytes and the growth of the fibroblast-like (diploid) or epithelioid (heteroploid) cell lines in tissue culture.

Animals↗
PubMed1980

Effect of the treatment of parental cells in vitro with anti-T antibody on bone marrow transplantation in F1 hybrid mice.

Anti-T antibody was obtained from xenogeneic antithymocyte serum by adsorption and elution with brain immunosorbent. Bone marrow and spleen cells from the parental A strain were treated in vitro with anti-T antibody and complement or with the original ATS and complement. So treated cells were assayed for the ability to induce a local and a total GVH reaction in (A X C57BL)F1 hybrid mice, to prolong the survival of lethally irradiated, identical F1 hybrid mice and to form haemopoietic colonies in spleens of the syngeneic irradiated recipients. The local GVH reaction induced by anti-T antibody- and ATS-treated spleen cells was somewhat lower. Administration of a mixture of spleen and bone marrow cells treated with anti-T antibody prolonged the survival of lethally irradiated recipients but did not prevent the consequences of late GVH reaction. The colony-forming activity of bone marrow cells treated with anti-T antibody was not reduced in most experiments. It can be concluded that the treatment of cells from mouse haemopoietic organs with anti-T antibody and complement damages mature T lymphocytes and in this way reduces the ability to induce GVH reaction, but does not damage T lymphocyte precursors and haemopoietic stem cells.

Animals↗
PubMed1979

Lymph node activation by a factor released from lymphocytes of normal mice in reaction with Mycobacterium kansasii.

Substances which have the same properties as the previously described lymph node activating factor are released from the 4-h culture of 10 X 10(6) lymph node cells from normal non-sensitized mice and 2.5--5 X 10(6) Mycobacterium kansasii cells. They increase the number of lymphocytes with nucleoli synthesizing ribonucleic acid in the popliteal lymph nodes of intact mice and give the precipitation lines with the beta- to alpha-globulin electrophoretic mobility, which are specific for the lymph node activating factor, in reaction with serum against the supernatants from mixed lymphocyte cultures. The conclusion drawn from these observations is that the release of the factor or related substances is one of the more general manifestations of the early response of lymphocytes to foreign cells. The activating and in immunoelectrophoresis specifically reacting substance is not released from lymphocytes of mice from a specific pathogen-free colony. We assume that the release of the factor is not the primary reaction of the non-sensitized lymphocytes but the secondary reaction of lymphocytes presensitized with substances which share antigenic determinants with Mycobacteria.

Animals↗
PubMed1979

An attempt at isolation and partial characterization of specific antibodies from antithymocyte serum.

Specific anti-T antibody was isolated from rabbit anti-mouse and anti-rat thymocyte antiserum on immunosorbent prepared by fixation of brain homogenate in glutaraldehyde. Conditions of isolation, purity of obtained antibody and cytotoxic activity were followed. The resulting preparation containing immunoglobulin G is contaminated with beta globulin and albumin, contains non-precipitating proteins originating from brain tissue and is cytotoxic for thymocytes. Cytotoxicity for bone marrow cells was not demonstrated.

Animals↗
PubMed1977

Immunoprecipitation and radioimmunological studies of a lymph node activating factor released during contact of H-2-or HLA-different lymphocytes.

Immunoprecipitation studies showed that the supernatants from the 4-hour mixed cultures of allogeneic mouse lymphoid cells contained a specific component with the alpha2-globulin electrophoretic mobility which can correspond to, or to which a "lymph node activating factor" can be bound. In addition, during allogeneic cell culture serum proteins with the prealbumin mobility were released into the culture medium and in the control culture of cells from only one donor proteins with the albumin mobility predominated. In the supernatants from the 4-hour cultures of human HLA-identical and HLA-diferent lymphocytes beta2-microglobulin was determined by immunodiffusion and radioimmunologically.

Alpha-Globulins↗
PubMed1976

Some biological properties of mouse spleen cells fractionated by the adherence of Sephadex G 25 and glass bead columns.

The possibilities of separation of haemopoietic cells from lymphocytes capable of eliciting the graft-versus-host reaction through column chromatography were investigated. Strain-A mouse spleen cells were fractionated into the adherent and non-adherent fraction on Sephadex G-25, glass bead columns and glass beads coated with antibody against mouse globulin. Increased numbers of cells forming haemopoietic colonies were found in the cell fraction which did not adhere to the antibody-coated glass beads and in cells reversibly adhering to glass beads. No significant decrease in local graft-versus-host reaction was found in any fraction obtained, and the prolonged survival of irradiated semiallogeneic recipients was observed in both fractions obtained on Sephadex G-25 columns.

Animals↗
PubMed1976

Asimple test for immunogenicity of colloidal infusion solutions- the draining lymph node activation.

The increasing number of lymphocytes with nucleoli synthesizing RNA in the mouse lymph nodes, draining the site of injection of infusion solutions, was used as a marker for their immunogenicity. The percentage of "active" lymphocytes significantly increased 3 days after the administration of preparations based on bovine serum or human haemoglobin which were found immunogenic when testing for antibody formation. Such a reaction was not elicited in mice treated with Physiogel, Dextran and Duxon which do not cause any production of antibodies. The described test may serve as a rapid and economical assay for the immunogenicity of infusion solutions and other substances.

Animals↗