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Biomedical subjects

E Okon

Publications and source records attributed to E Okon.

At least 127 records · Page 7Linked to original sources
PubMed1986

Histamine and chondroitin sulfate E proteoglycan released by cultured human colonic mucosa: indication for possible presence of E mast cells.

An association between the release of histamine and chondroitin sulfate E proteoglycan (PG) was demonstrated in human colonic mucosa (HCM). Colonic biopsy samples incorporated [35S]sulfate (2.7 X 10(6) +/- 188 X 10(3) cpm/mg of wet tissue; mean +/- SEM, n = 5) into PG, which was partially released into the culture medium during the incubation period. Ascending thin-layer chromatography of the released 35S-labeled PG after its digestion by chondroitin ABC lyase (chondroitinase, EC 4.2.2.4) followed by autoradiography yielded three products that migrated in the position of monosulfated disaccharides of N-acetylgalactosamine 4-sulfate and N-acetylgalactosamine 6-sulfate and of an oversulfated disaccharide possessing N-acetylgalactosamine 4,6-disulfate. Cultured colonic mucosa released 23.6 +/- 3.7 ng of histamine per mg of wet tissue (mean +/- SEM, n = 16) without any specific trigger. Comparison by linear regression analysis of the release of histamine and chondroitin [35S]sulfate E PG revealed a correlation coefficient (r) of 0.7 (n = 16; P less than 0.005). Histological examination of the colonic biopsies revealed the presence of many mast cells in various degrees of degranulation in the mucosa and submucosa, most of which were found in the submucosa. Incubation of the HCM biopsies in the presence of anti-human IgE revealed 58% +/- 12% (mean +/- SEM, n = 3) enhancement in the release of chondroitin [35S]sulfate E PG and 64% +/- 10% (mean +/- SEM, n = 4) of histamine release. The above correlation, the observation that most of the mast cells showed various degrees of degranulation, and the lack of heparin synthesis as opposed to the synthesis and immunological release of chondroitin sulfate E strongly suggest that the E mast cell exists in the human colon.

Cells, Cultured↗
PubMed1986

Urticarial vasculitis and recurrent pleural effusion: a systemic manifestation of urticarial vasculitis.

A 62-year-old woman with an 8-year history of urticaria, arthralgia and arthritis developed recurrent pleural effusion. A thorough laboratory workup during repeated attacks of concomitant urticaria and pleural effusion disclosed only hypocomplementemia and the histopathological finding of the skin biopsy revealed leukoclastic vasculitis. These findings are compatible with the entity of urticarial vasculitis. It is thus suggested that pleural effusion can form part of the systemic manifestations of urticarial vasculitis.

Complement Pathway, Classical↗
PubMed1986

Localization of growth hormone-releasing hormone in the human hypothalamus and pituitary stalk.

The localization of the recently identified GH-releasing hormone (GHRH) in the human hypothalamus and pituitary stalk was determined by microdissection techniques and a specific RIA for GHRH. The highest concentrations of GHRH immunoreactivity (IR-GHRH) in the hypothalamus were found in the area of the infundibular nucleus (83 +/- 4 ng/mg protein; average +/- range). Lower quantities were found in other hypothalamic regions. Very high concentrations of IR-GHRH were present in the upper portion of the pituitary stalk (1454 +/- 48 ng/mg protein), and they decreased gradually toward the distal end of the stalk (21 +/- 3 ng/mg). This concentration gradient suggests that the peptide reaches the anterior pituitary mainly by way of the long portal vessels. Somatostatin, the second neuropeptide involved in the regulation of GH secretion from the anterior pituitary, had a pattern of distribution along the pituitary stalk very similar to that of IR-GHRH.

Adult↗
PubMed1986

Cytomegaly in benign ovarian cysts.

We report two cases of benign ovarian cysts that revealed cytomegaly of the epithelial lining cells. One case was a large solitary luteinized follicle cyst of pregnancy and puerperium. The other case was an endometrial cyst. Changes in the cells included marked pleomorphism and hyperchromasia of the nuclei, large amounts of cytoplasm, and lack of mitotic figures. We ascribed these cellular features to hormonal effects and stress the importance of recognizing such changes and differentiating them from malignancy.

Adult↗
PubMed1986

Pouch ileitis--recurrence of the inflammatory bowel disease in the ileal reservoir.

"Pouchitis" is an inflammation of the ileal pouch that has been described as occurring after Kock's procedure--the continent ileal pouch. The case presented herein demonstrates clinical and histological features that resemble ulcerative colitis, implying that this disorder may be a recurrence of the patient's original inflammatory bowel disease and not merely a local and nonspecific process as previously suggested.

Adult↗
PubMed1985

Monoclonal antibodies reactive with B-lymphocytes and histiocytes in paraffin sections.

Representative sections of normal lymph nodes, cases of reactive lymphadenopathy, and Hodgkin's disease were examined using two monoclonal antibodies reactive in paraffin sections using an immunoperoxidase technique. Antibody LN-1 recognizes B-lymphocytes in follicles; antibody LN-2 recognizes a broader spectrum of B-cells, and also shows positivity with some monocytes/histiocytes. The pattern of immunostaining with these antibodies is described with particular reference to Hodgkin The pattern of immunostaining with these antibodies is described with particular reference to Hodgkin and Reed-Sternberg cells. Some of the implications are discussed.

Antibodies, Monoclonal↗
PubMed1985

Secondary myelofibrosis with metastatic breast cancer simulating agnogenic myeloid metaplasia: report of a case and review of the literature.

A patient with carcinoma of the breast, who developed a clinical syndrome resembling agnogenic myeloid metaplasia, is described. She was found to have secondary myelofibrosis with metastatic carcinoma of the marrow, extensive extramedullary hematopoiesis resulting in massive splenomegaly, and leukoerythroblastosis. These findings are rare and myeloid metaplasia with striking extramedullary hematopoiesis causing massive splenomegaly and mimicking agnogenic myeloid metaplasia is seldom seen in patients with metastatic carcinoma. Previous case reports of secondary extramedullary hematopoiesis simulating agnogenic myeloid metaplasia (AMM) in patients with solid tumors are also reviewed.

Adult↗
PubMed1985

Should we worry about gastric cancer in duodenal ulcer patients?

In three patients with active duodenal ulcer, carcinoma of the stomach was detected early during routine endoscopic examination. In all patients subtotal gastrectomy was performed, and pathological examination of the resected stomach revealed carcinoma confined to the mucosa. We review evidence for and against any association between gastric carcinoma and peptic ulcer disease.

Adenocarcinoma↗
PubMed1985

Coexistence of Kaposi's sarcoma and chronic lymphocytic leukemia in the same lymph node.

A patient with chronic lymphocytic leukemia in whom a carcinoma of the gall bladder was previously found is described. The patient was treated with prednisone and chlorambucil and developed a significant lymphadenopathy. A biopsy of one of the lymph nodes revealed the coexistence of Kaposi's sarcoma and chronic lymphocytic leukemia in the same node. This and the presence of three malignancies in the same patient are discussed.

Aged↗
PubMed1985

A new human ovarian carcinoma cell line: establishment and analysis of tumor-associated markers.

In the present study we describe the establishment and characteristics of a new human tumor cell line (OV-1063) positive for carcinoembryonic antigen (CEA) originating from ovarian metastatic tumor cells. Analysis of the cultured cells during their in vitro adaptation period revealed while the primary culture exhibited a low proportion of CEA-positive cells, this proportion increased with culture passages and eventually more than 90% of the cells in the established line were CEA-positive. Thus, during the period of adaptation to in vitro growth, a selection for CEA-positive cells took place but the amount of CEA secreted per each positive cell seemed to be constant. Several tumor-associated characteristics were found positive on the established OV-1063 cell line. The in vitro growing cell line exhibited an abnormal chromosome pattern with a near-trisomy karyotype for some chromosomes, colony formation in soft agar as well as positive staining with a monoclonal antibody B38.1. Culture supernatants of the OV-1063 cells contained significant amounts of CEA as well as CA-125 antigen which is an ovarian-carcinoma-associated antigen.

Agar↗
PubMed1984

Familial hemophagocytic lymphohistiocytosis in Israel. II. Pathologic findings.

Presented are the pathologic findings in familial hemophagocytic lymphohistiocytosis (FHLH), based on observation of 11 cases from four affected families. The outstanding morphologic feature was a multiorgan involvement with lymphohistiocytic cellular infiltrates. The organs most frequently affected were the bone marrow and lymph nodes and, less frequently, the liver and the brain. The histiocytes in the infiltrates were usually bland and displayed prominent hemophagocytosis. The differential diagnosis of FHLH and the difficulty in distinguishing it from malignant histiocytosis is discussed. In view of the focal distribution of lesions, it is suggested that histopathologic diagnosis of FHLH be made only after evaluation of combined findings in several organs and consideration of the patient's family history as well as the result of the clinical and laboratory investigations.

Bone Marrow↗
PubMed1984

Generalized lymphadenitis following B.C.G. vaccination in an immunocompetent 12-year-old boy.

An immunocompetent, healthy 12-year-old boy was presented with generalized large lymphadenopathy in the left side of the body, 1 month after a BCG vaccination with a regular dose. A Mantoux test before vaccination was negative. Histological and bacteriological findings from the largest lymph node biopsy revealed BCG'itis. The patient recovered spontaneously after 7 months. He was confirmed as being immunocompetent by normal serum immunoglobulin levels, normal B and T cell function tests, and a conversion of Mantoux test. BCG infections including lymphadenitis are rare and have been described hitherto mainly in infants, in immune deficient patients, or related to overdose. Such a complication must be taken into consideration when dealing with lymphadenopathy in healthy school-age children, who now undergo BCG vaccinations in many countries.

B-Lymphocytes↗
PubMed1984

Cell adhesiveness is related to tumorigenicity in malignant lymphoid cells.

Mouse lymphoma cells (S49) that grow in suspension culture were selected for increased tumorigenicity through continuous passages in syngeneic BALB/c mice. Developing tumors were classified as high grade malignant lymphoma, small noncleaved type. Variants were selected from these tumorigenic cells that were able to grow as a monolayer attached to their substrate, resembling, in this respect, fibroblastoid cells. Whereas the tumorigenic suspension-growing parental cells were able to induce progressive tumors with an inoculum as low as 100 cells per mouse, the adherent cells were unable to develop as tumors even at an inoculum of 1 X 10(8) cells per mouse. In addition, mice inoculated once with live adherent cells were immunized against 1 X 10(7) suspension-growing cells. Involvement of an immune response in the rejection of tumorigenic S49 cells was suggested by (a) adoptive transfer experiments in which spleen cells from immunized mice protected naive mice and (b) the appearance of antibodies in the sera of immunized syngeneic mice that specifically recognized both adherent and suspension-growing S49 cells and detected differences in [35S]methionine-labeled antigens from these cells. Antibodies raised in rabbits against adherent cells recognized three proteins of 34,000, 61,000, and 72,000 apparent molecular weight in radiolabeled adherent cell extracts that are either absent or present in small amounts in extracts of suspension-growing tumorigenic S49 cells. These findings, taken together with our previous report (Hochman, J., A. Katz, E. Levy, and S. Eshel, 1981, Nature (Lond.), 290:248-249), suggest the S49 system as a novel system for studying growth control in malignant lymphoid cells.

Animals↗
PubMed1984

Disseminated intravascular coagulation in two patients with histiocytic medullary reticulosis.

Two young adults with characteristic features of histiocytic medullary reticulosis (HMR) are described. Of particular interest was the development of striking clinical and laboratory features of disseminated intravascular coagulation (DIC), in the absence of sepsis in both cases. Both patients had a marked and fatal bleeding tendency. The association between neoplasia and DIC is well established, but, to the best of our knowledge, DIC presenting a major feature of HMR has not yet been described. The possible pathogenesis of DIC in HMR is discussed.

Adult↗