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Biomedical subjects

E NETER

Publications and source records attributed to E NETER.

At least 19 recordsLinked to original sources
PubMed1964

SEPARATION BY ETHANOL OF COMMON AND SOMATIC ANTIGENS OF ENTEROBACTERIACEAE.

Suzuki, T. (Children's Hospital, Buffalo, N.Y.), E. A. Gorzynski, and E. Neter. Separation by ethanol of common and somatic antigens of Enterobacteriaceae. J. Bacteriol. 88:1240-1243. 1964.-Ethanol in a concentration of 85% permits separation from crude culture supernatant fluids of the common enterobacterial antigen and the O antigen, the former being ethanol-soluble, the latter being ethanol-insoluble. The evidence was obtained in hemagglutination, hemolysis, and hemagglutination-inhibition tests. In contrast to the crude antigens obtained from enteric bacteria other than Escherichia coli O14, the ethanol-soluble fraction is immunogenic in the rabbit upon intravenous injection, and antibodies against the common antigen are produced in relatively high titers. Aqueous mixtures of ethanol-soluble and -insoluble fractions engender antibodies against the common antigen in titers significantly lower than those stimulated by the soluble fraction alone. Ethanol-treated whole antigen fails to stimulate antibody formation against this antigen. These results suggest the presence of an inhibitor in the ethanol-insoluble fraction.

Animals↗
PubMed1964

SELECTIVE DESTRUCTION BY PSEUDOMONAS AERUGINOSA OF COMMON ANTIGEN OF ENTEROBACTERIACEAE.

Whang, H. Y. (Children's Hospital, Buffalo, N.Y.), and E. Neter. Selective destruction by Pseudomonas aeruginosa of common antigen of Enterobacteriaceae. J. Bacteriol. 88:1244-1248. 1964.-Supernatant fluids and filtrates from several strains of Pseudomonas aeruginosa, when incubated with common antigen from various enteric bacteria, will produce selective destruction of this antigen, leaving intact the simultaneously present O antigen. This effect is evidenced from hemagglutination, hemolysis, antibody absorption, hemagglutination inhibition, and immunization experiments. The Pseudomonas factor is heat-labile, being destroyed by heating at 100 C for 10 min. The action of this factor on the antigen is time-dependent and temperature-dependent, requiring incubation for 18 to 72 hr and progressing more rapidly at 50 or 56 C than at 37 C. The specificity of the reaction is indicated by the fact that supernatant fluids from cultures of Staphylococcus aureus and Bacillus subtilis, as well as several enzymes, such as pancreatic protease, trypsin, and lipase, have no effect on the common antigen. Utilization of the Pseudomonas factor, conceivably an enzyme, may aid in the eventual elucidation of the chemical nature of the common antigen of enteric bacteria.

Antigens↗