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Biomedical subjects

E Mussini

Publications and source records attributed to E Mussini.

101 records · Page 6Linked to original sources
PubMedDate not supplied

Plasma levels of chlorodesmethyldiazepam in humans.

Chlorodesmethyldiazepam (I) concentrations were followed for 72 h in the plasma of four volunteers given 2 mg of the drug orally. The drug appears to be well absorbed, reaching peak plasma levels of 20--30 ng ml-1 1--2 h after administration; 72 h after administration, plasma concentrations are still measurable (5 ng ml-1). The analytical method involved a single extraction of I from the plasma into benzene followed by centrifugation, evaporation, and gas-chromatographic analysis of the samples.

Administration, Oral↗
PubMedDate not supplied

Changes in muscle esterases in genetically dystrophic and control littermate mice.

The hypothesis that the existence of a family of isoenzymes is at the basis of the heterogeneity of esterase activity among skeletal muscles from dystrophic Re 129J and control littermate mice was tested using isoelectrofocusing (IEF) in polyacrylamide gel. All muscles considered showed not only quantitative heterogeneity, as previously observed in this laboratory, but also qualitative differences with regard to their esterase isoenzymes. Differences in the number and the relative amounts of isoenzymatic bands were found between different muscles from both control and dystrophic animals. In most dystrophic muscles a new isoenzymatic band appeared at pH 6.6. Another band, identified at pH 5.12, was more pronounced in dystrophic than in control muscles. The specificity of these observations is indirectly supported by the fact that other tissue, such as heart, liver, and kidney, did not show the quantitative or qualitative abnormality present in the dystrophic skeletal muscles.

Animals↗
PubMedDate not supplied

Content of methylhistidines in normal and pathological human skeletal muscles.

The content of 3-methylhistidine (3-MH) and 1-methylhistidine (1-MH) was measured in muscle biopsy specimens from 13 normal controls, 19 patients with Duchenne muscular dystrophy, 8 limb-girdle disease patients, and 23 disease controls with different forms of muscular pathology. 3-MH and 1-MH concentrations in normal human muscle did not appear to be influenced by sex, body weight, and age, at least for subjects in the 10--60 year age group examined. Skeletal muscle 1-MH levels did not significantly differ from mean control values in any of the pathologies investigated. In the patient population examined, the mean 3-MH level per unit of noncollagen protein (NCP) was significantly lower than normal in Duchenne dystrophy only, the reduction being related to disease severity. The significantly lower concentrations of 3-MH in muscle of Duchenne patients indicate the importance of measuring 3-MH in diseased muscle to obtain reliable estimates of the myofibrillar protein catabolic rate.

Adolescent↗
PubMedDate not supplied

Lactose malabsorption and recurrent abdominal pain in Italian children.

The role of lactose malabsorption (LM) was investigated in 32 children (mean age 8.13 +/- 2.46 years) with recurrent abdominal pain (RAP). LM was detected in 75% of them by a lactose breath hydrogen test (LBHT) after a 2-g/kg (max 50-g) load. Of the 18 malabsorbers who participated in a 3-month lactose-free diet (LFD), 14 were judged "improved" and reported lower pain frequency (p less than 0.001). The malabsorbers who improved versus the not improved had comparable past lactose ingestion but were distinguishable on the basis of their lactose absorption capacity (0.36 vs. 0.81 g/kg; p less than 0.01), as subsequently determined by multiple LBHTs with 25-, 12.5-, and 6-g loads. The ratio between past lactose ingestion and lactose absorption was 1.89 in the improved and 0.55 in the not improved groups (p less than 0.01), retrospectively indicating lactose as a possible cause of the symptoms in the improved group. The reintroduction of lactose in amounts not exceeding the absorption capacity into the diet of each malabsorber who had improved with LFD caused relapse in none of the 14 subjects monitored for 2-6 months. In conclusion, LM seems an important cause of symptoms in Italian children with RAP. Assessment of the lactose absorption threshold of each subject of LBHTs provides a basis for reintroduction of "calibrated" amounts of lactose-containing foods (e.g., milk) into the diet.

Administration, Oral↗
PubMedDate not supplied

Further studies on rat, mouse and guinea pig muscle esterase activity.

Esterase activity was investigated in several skeletal muscles of different rodent species employing as substrates a series of alpha and beta naphthyl esters of different aliphatic chain lengths. Results with these chemically homogenous substrates show there is marked heterogeneity in the levels of esterase activity in several skeletal muscles of the same animal and among the same muscles from different species depending also on the sex, and the type and stereospecificity of the substrate.

1-Naphthylamine↗
PubMedDate not supplied

Esterase activity in the skeletal muscles of dystrophic and normal mice.

Esterasic activity was measured in several muscles of dystrophic and littermate control Re 129/J mice employing as substrates a series of alpha and beta naphthyl esters of different aliphatic chain length (C2 to C5). Different skeletal muscles of normal and dystrophic mice varied markedly in their esterase activity when tested not only with different, closely related substrates but also with the same substrate. Dystrophic skeletal muscles showed higher esterase activity than the corresponding muscles of controls. No increase in enzymatic levels was seen in the heart and parenchymatous organs of dystrophic hosts.

1-Naphthylamine↗