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Biomedical subjects

E Mix

Publications and source records attributed to E Mix.

71 records · Page 4Linked to original sources
PubMed1988

[The binding behavior and functional significance of Fc receptors for IgG on T lymphocytes of the mouse].

In consequence of allogenic stimulation of mouse spleen lymphocytes the binding of fluorescein isothiocyanate conjugated aggregated IgG (FITC-aggr. IgG) per cell is increased (higher affinity of receptors and/or higher number of receptors). A higher specificity of the FITC-aggr. IgG binding to allogeneically stimulated lymphocytes in comparison to fresh prepared resting and syngeneically cultivated lymphocytes could be only detected on the higher fluorescing allogeneically stimulated cells. Increased amount of Fc-receptors for IgG (Fc gamma R) is mainly found on T-lymphocytes with the Lyt-1 + 2 +-phenotype. The manipulation of that Fc gamma R on T-lymphocytes, which were expressed in consequence of allogeneic stimulation by binding of respective ligands, did not change the original effect of nonmanipulated, allogeneically activated cells on a primary MLR. But if the cells were negatively selected by means of monoclonal antibodies and complement, the originally suppressive effect of allogeneically stimulated Lyt-2 positive cells on a primary MLR was abolished by manipulating the respective Fc gamma R.

Animals↗
PubMed1987

Phagocytic activity of CSF monocytes in neurological diseases.

A standard latex phagocytosis test was used to determine the phagocytic activity of CSF monocytes under various pathological conditions. The mean values for groups of neurological diseases did not differ significantly, with the exception of tumour patients. Differences in the phagocytosis of CSF monocytes were seen if subdivisions within the groups of patients with multiple sclerosis (MS) and vertebral irritation syndrome (VIS) were evaluated separately. The active stage of MS and the intervertebral disc prolapse were accompanied by higher values. It is concluded that although the findings are of no use for diagnostic purposes they may be helpful in assessing prognosis and therapy.

Humans↗
PubMed1986

Flow cytometric estimation of transmembrane potential of macrophages--a comparison with microelectrode measurements.

Potential-dependent accumulation of the lipophilic cationic dye 3,3' dihexyloxacarbocyanine (DiOC6(3)) in macrophages has been investigated. Resulting fluorescence of cells was measured by flow cytometry. Alterations of membrane potential of macrophages were induced by ionophore treatment (valinomycin and gramicidin) in a dose-dependent (10(-5) M-10(-7) M) and time-dependent (0 min-45 min) manner. Resulting changes in relative fluorescence intensity were compared with changes of transmembrane potential measured by intracellular recordings obtained by applying glass microelectrodes. The comparative studies offer the possibility to calibrate the flow cytometric estimate of membrane potential of suspended cells. Equilibration of dye partition between cells and surrounding medium is strictly potential-dependent at dye concentrations between 5 X 10(-8) M and 10(-7) M and within an incubation interval from 10 min up to 30 min after addition of dye. Conclusions are drawn concerning the field of application of the optical method. Dynamics of electrical processes following ionophore treatment are discussed in terms of molecular mechanisms of altered ionic transport.

Animals↗
PubMed1985

[Heterogenous immune reactivity in brain tumors: results of cellular immunity in relation to brain tissue antigens].

In brain tumors and other neurological diseases cell-mediated immune reactions to fetal brain tissue antigens (FBA), normal tissue antigen of adult brain (NTA) and tumor-associated antigens of different brain tumors (TAA) have been analysed. The detection of sensitized lymphocytes using the MEM-(macrophage-electrophoretic-mobility-) test revealed general tumor-related results applying the FBA, in some extent a cross reactivity and partly no kind of reaction. A phase-specific reactivity to normal brain antigens could not be found, only cases of multiple sclerosis produced restrictive results employing the NTA. By testing tumor-associated brain antigens different reaction types were seen: The common TAA caused a tumor-characteristic reaction; the histo-specific TAA predominantly presented a organotypic form of reaction, confined a histo-specific reaction pattern; in some cases there were found inadequate, non-corresponding reactions as well as unreactivity. With regard to different types of reaction the problems of heterogeneity of the brain tumors and the cellular immune response--i.e. a heterogeneity of 1. or 2. order--were discussed including further factors concerning several special conditions in the nervous system.

Antigens, Neoplasm↗
PubMed1984

The nonsurgical treatment of microstomia in scleroderma: a pilot study.

A 6-month clinical trial with nine scleroderma patients compared two exercise programs for the nonsurgical treatment of the decreased oral opening ( microstomia ). The experimental group employed three mouth-stretching exercises and a new oral augmentation exercise, using tongue depressors , to obtain a 5.6 mm mean increased vertical oral opening. The controls used six currently recommended "facial grimacing " exercises for a 3.0 mm mean increase. When the controls used the experimental treatment, their mean improvement increased to 5.7 mm. Although surgical correction of microstomia may be warranted, some reversal of this condition may be achieved with the aid of this new nonsurgical exercise program.

Double-Blind Method↗
PubMed1984

Flow cytometric measurements of phagocytosis. I. A methodical and comparative study.

A flow cytometric technique is described for measurement of phagocytic activity in human leucocytes using FITC-labelled latex particles (0.77 micron). Cell suspensions separated from peripheral blood by dextran sedimentation were hemolyzed to avoid particle uptake by erythrocytes. The leucocytes were incubated for various time intervals with a standardized solution of 2 x 10(7) FITC-labelled latex particles per 2 x 10(5) leucocytes in 0.6 ml medium. Relative fluorescence per cell was measured on the basis of 10(5) cells, and a simple technique has been described to evaluate data characteristic for different steps of phagocytosis and to discriminate between phagocytizing and non-phagocytizing cells. In comparison, rice starch phagocytosis has been measured microscopically. A positive correlation between both methods has been found in 11 patients with different disease-related phagocytic capacity.

Erythrocytes↗
PubMed1984

Immunological reactivity of tumor associated glycoproteins prepared by affinity chromatography.

Crude 3M KCl extracts prepared from tumor tissue and ascitic tumor cells of methylnitrosourea-induced fibrosarcoma of CBA mice or of a human stomach adenocarcinoma were gelfiltrated on Ultrogel AcA 34. Single fractions of these preparations were incubated with spleen cells of tumor bearing mice or blood peripheral lymphocytes. The response was detected by MEM-technique. Antigenic activities were found in molecular weight ranges from 200 to 300 kD (P1) and from 40 to 70 kD (P2). Single glycoproteins of the P1- and P2-fractions could be separated by affinity chromatography on immobilized D-galactose specific mistletoe lectin I, as detected by electrophoresis on microgradient gels (1.2-40%) or on SDS polyacrylamide slab gels. Especially the isolated glycoproteins of the P1-fractions are responsible for the observed antigenic reactivity, assayed in vitro by the MEM-technique and in vivo by foot pad swelling test.

Adenocarcinoma↗
PubMed1982

Histamine-receptor bearing lymphocytes. II. The use of flow cytofluorometric analysis of histamine-receptors on mouse lymphocytes of different organs.

The histamine binding capacity of cells in various lymphoid organs has been determined by flow cytofluorometric (FCF) analysis of histamine receptors. Using fluoresceinated bovine serum albumin conjugated to histamine (HIS-BSA-FITC) as a staining reagent two distinct lymphocyte subpopulations of differing fluorescence intensity have been identified in each organ investigated. The thymus has a low number of histamine-receptor bearing cells, while bone marrow, lymph nodes and spleen reveal increasing binding capacities to the histamine conjugate. It is suggested that there exists a different susceptibility of distinct lymphocyte subpopulations for histamine, thereby enabling its controlled immunoregulatory activity.

Animals↗
PubMed1976

[Behavior of thymus-arised lymphatic cells in the peripheral blood in acute leukoses].

On the basis of their ability of the spontaneous formation of rosettes by unsensibilised sheep erythrocytes the proportion of thymic lymphatic cells in 7 acute and 3 chronic lymphatic leukaemias was determined. Further observations on the content of T-cells in the peripheral blood were performed in leukoses, whereby the remission was begun with prednisone and cytostatics, under the immune stimulation with BCG during the remission and in 3 children with lymphogranulomatosis before and after splenectomy. The results of the acute stage of leucosis refer to the possibility of a prognostic evidence with the subclassification of the ALL. The T-cell-leukaemias revealed a particularly unfavourable course of disease. Cytochemically they were acute lymphatic leukaemias of undifferentiated type. In leucoses with smaller proportion of T-cells the blasts above all revealed a granular PAS-reaction.

Animals↗
PubMedDate not supplied

Effect of monoamine reuptake inhibiting antidepressants on major histocompatibility complex expression on macrophages in normal rats and rats with experimental allergic neuritis (EAN).

This study examined the modulation of IFN-gamma induced MHC class I and II expression on normal Lewis rats and rats with EAN peritoneal macrophages cultured in the absence or presence of 10(-4)-10(-8) M of the 5-HT reuptake inhibiting antidepressants zimeldine, and its metabolites norzimeldine and cpp200 oxalate as well as the antidepressants clomipramine and imipramine, in addition amitriptyline, nortriptyline and maprotiline in EAN rats. In normal rats, MHC class I expression was suppressed by the antidepressants zimeldine, norzimeldine and cpp200 oxalate at concentrations up to 10(-5) M. At concentrations between 10(-6) to 10(-8) M, the same drugs significantly enhanced MHC class expression. Clomipramine at 10(-8) M and imipramine at 10(-6)-10(-7) M enhanced MHC class I expression, while the MHC class II expression was not significantly influenced by concentrations < or = 10(-5) M of these two drugs. In EAN rats, MHC class I expression was enhanced by zimeldine, cpp200, imipramine, and nortriptyline at 10(-5)-10(-8) M, amitriptyline at 10(-5)-10(-7) M as well as by norzimeldine and clomipramine at 10(-6) M-10(-8) M. However, maprotiline at 10(-4)-10(-6) M suppressed class I expression in the presence of 0.5 U/ml and 1.0 U/ml of IFN-gamma. MHC class II expression was suppressed by cpp200 and clomipramine at 10(-4)-10(-5) M in presence of 0.5 U/ml of IFN-gamma. At concentrations < 10(-5) M most tested drugs significantly enhanced IFN-gamma induced MHC class II expression. Compared to the results in normal rats, drug effects on EAN macrophages were more pronounced and reached higher levels of significance. The 5-HT reuptake inhibiting antidepressants also exerted a modulatory effect on MHC class I and II in EAN rat macrophages even in the absence of IFN-gamma. The modulatory effect of antidepressant drugs on IFN-gamma induced MHC class I and II expression may contribute to their influence on demyelinating autoimmune diseases, and may have implications for their clinical use.

Amitriptyline↗