Biomedical subjects

E Mitsuzawa

Publications and source records attributed to E Mitsuzawa.

4 recordsLinked to original sources

PubMed1984

Pretreatment with antigen or adjuvant, and the preventability of allergic encephalomyelitis induced by basic protein with synthetic muramyl dipeptide in comparison with Freund's complete adjuvant.

Protection against experimental allergic encephalomyelitis (EAE) was examined by prior injection of following materials with Freund's incomplete adjuvant (FIA), Mycobacterium tuberculosis (Tbc), synthetic muramyl dipeptide (Mdp), polyinosinate-polycytidylate (poly IC), or myelin basic protein (BP). 26 days later, guinea pigs received encephalitogenic challenge in the form of 20 micrograms BP + 20 micrograms Tbc in FIA (BP + Tbc) or 20 micrograms BP + 10 micrograms Mdp in FIA (BP + Mdp). (1) Either pretreatment with Mdp, poly IC or FIA alone showed no protecting effects. (2) Tbc/FIA was not effective on challenge with BP + Mdp, but effective on challenge with BP + Tbc, and produced passive hemagglutinating (PH) antibodies of moderate titer and and complement fixing (CF) antibodies of higher titer. (3) BP/FIA was effective on both BP + Tbc and BP + Mdp challenges, and produced PH antibodies of higher titer than that of CF antibodies. (4) Clinicopathological survey revealed that the challenge with BP + Mdp was blocked perfectly by prior injection with BP/FIA whereas the challenge with BP + Tbc was incompletely blocked by either Tbc/FIA or BP/FIA pretreatment. (5) The finding was verified also by cerebrospinal fluid cell count at the termination of clinical observation. As compared with FCA, Mdp adjuvant itself has no antigenic component, which seems to explain the results in this study.

Acetylmuramyl-Alanyl-Isoglutamine↗

PubMed1981

Experimental allergic encephalomyelitis induced by basic protein with synthetic adjuvant in comparison with Freund's complete adjuvant. Role of antibodies in correlation with the clinicopathological features.

Experimental allergic encephalomyelitis (EAE) was induced in guinea pigs with bovine myelin basic protein (BP) with adjuvant of either synthetic muramyl dipeptide (Mdp) or Mycobacterium tuberculosis (Tbc). The following results were obtained: (1) The body temperature of the animals was studied serially after sensitization and its elevation was shown to be an early sign of EAE. (2) Several animals developed the clinical and histological signs of hyperacute EAE. (3) An optimal combined dosage of BP and adjuvant was found for induction of clinical EAE and for the the production of complement fixing (CF) antibodies. (4) Little passive hemagglutinating (PH) antibody was produced by single immunization. These results displayed no essential difference in EAE induced by either adjuvant. (5) Detectable PH antibodies developed later in addition to CF antibodies in a few animals immunized with Tbc adjuvant. These animals were skin-tested to BP, and had recovered from body weight loss or limb weakness. The results suggest that humoral antibodies play a role in modifying the disease process, even if they are not essential in production of EAE.

Acetylmuramyl-Alanyl-Isoglutamine↗

PubMed1976

Experimental allergic encephalitis (EAE) in mice: histological studies on EAE induced by myelin basic protein, and role of pertussis vaccine.

The histological evidence of EAE in mice was presented to show the identity with those of other species of animals. There was a typical perivascular mononuclear cell infiltration. In addition, a hyperacute form was characterized by edema and the infiltration of polymorphs. In vascular lesions, vascular stasis, thrombosis, and hemorrhage were observed. As for the effect of pertussis vaccine used in the induction of mouse EAE, responsiveness to the histamine sensitizing factor was not directly correlated to the incidence of EAE. However, the intravenous administration of pertussis vaccine caused remarkable leukocytosis, which might play a certain role in mouse EAE induction.

Animals↗

PubMed1975

Experimental allergic encephalomyelitis (EAE) in mice. I. Induction of EAE with mouse spinal cord homogenate and myelin basic protein.

The condition of experimental allergic encephalomyelitis (EAE) induction was investigated in several mice strains. SJL and C3H/He strains were found to be susceptible. A single immunization with mouse spinal cord, complete Freund's adjuvant (CFA) and pertussis vaccine produced clinical signs of EAE in SJL and C3H/He strains after 11 to 18 days. Isogenic spinal cord produced EAE in C3H/He strain. A single immunization with myelin basic protein from bovine spinal cord in CFA and pertussis vaccine produced EAE in SJL strain. EAE susceptibility of SJL strain correlated with the amount of mycobacteria used for sensitization. It was necessary to give pertussis vaccine intravenously in all cases.

Animals↗