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Biomedical subjects

E Michalak

Publications and source records attributed to E Michalak.

48 records · Page 3Linked to original sources
PubMedDate not supplied

Dilated cardiomyopathy in children: clinical course and prognosis.

The clinical profile of 19 patients with dilated cardiomyopathy ages 2-18 years (mean 13.4 +/- 4 years) was reviewed to detect any factors that might be predictive for their survival. Follow-up range from 5 to 105 months (mean 39 +/- 33 months). Routine treatment consisted of digitalis and diuretics: 14 patients received antiarrhythmics, 6 received vasodilators, and 12 were managed with immunosuppression. There were 12 survivors and 7 nonsurvivors: The 1-year mortality was 21.2% and the 2-year mortality 35.8%. All deaths were within first 2 years. Of the 12 patients who survived 2 years, a significant improvement was noticed in 9. In 3 patients tachycardia-induced cardiomyopathy was diagnosed, and abolition of supraventricular tachycardia was followed by improvement and regression of cardiomegaly. Endomyocardial biopsy was performed in 16 patients. Four with a histologic diagnosis of active myocarditis survived, and in 3 of them a considerable improvement was noticed. Of the 12 patients with nonspecific histologic findings, 6 died (p < 0.05). There were no significant differences between survivors and nonsurvivors for any of the following parameters: incidence of severe heart failure (NYHA class III-IV) and severe ventricular arrhythmias (Lown class III-V), relative heart volume, echocardiographic left ventricular diastolic diameter and shortening fraction, and the hemodynamic parameters of cardiac index, left ventricular ejection fraction, left ventricular end-diastolic pressure, and left ventricular end-diastolic volume index.

Adolescent↗
PubMedDate not supplied

FISH mapping of two putative keratin gene clusters on cat (Felis catus) chromosomes.

Genes encoding keratins are evolutionary highly conserved and clustered in two linkage groups in mammalian genomes. Canine keratin 9 (K-9) and keratin 2e (K-2) cosmid-derived gene probes were used to localize the acidic and basic-neutral keratin gene clusters to cat chromosomes E1q12 and B4q15, respectively. The status of the physical map of the cat genome is discussed.

Animals↗
PubMedDate not supplied

[Histopathologic differentiation of tumors derived from neuroblastic cells in children depending on age].

The histopathology of 224 tumours of neuroblastic origin was analysed in relation to the age of patients. Maturing neuroblastomas (NBS) dominated in infants, where as in the most numerous group of children 1-5 years of age, prevalence of neuroblastomas without any signs of maturation was noted. In children over 5 years of age a high incidence of tumours with evident maturation was noted (ganglioneuroblastomas and ganglioneuromas). The prevalence of maturing neuroblastomas in infants correlate well with an advantageous clinical course of neuroblastomas at this age.

Adolescent↗
PubMedDate not supplied

[Value of comparing flow cytometry examination and histopathology in prognosing of osteogenic sarcoma - preliminary report].

Our preliminary report presents two cases of osteogenic sarcoma examined after initial chemotherapy. In both cases histological maps with morphological effects of chemotherapy and flow cytometry of DNA content in tumour cells were made. Results of flow cytometric DNA histograms were linked with an estimation of the proliferative potential of tumours. The correlations between histological grade, proliferative potential of tumours with the morphological effects of chemotherapy were confirmed in the presented cases. Flow cytometry results were ready in a few hours after surgery, when full histopathological analysis was available in 2-4 weeks. Analysis of the two methods of estimation of osteogenic sarcoma suggests that flow cytometry may be a complementary method to histological examination and estimation of proliferating rate, especially aneuploid cell population and this information can be helpful in clinical management.

Adolescent↗
PubMedDate not supplied

[Difficulties in the histopathological evaluation of osteogenic sarcoma].

The performed analysis concerned the results of the histopathological examination of 169 cases of osteogenic sarcomas with reference to diagnostic difficulties. It was found that the most difficult cases belong to the extreme groups of the classification i.e. were high or very low differentiated osteogenic sarcomas. The most difficult diagnostically cases according to the structural classification concern analogically the extreme group of cases of the non-conventional histology. However, the dominant number of the diagnosed cases did not belong to the extreme groups of classification.

Adolescent↗
PubMedDate not supplied

[Comparative study of histopathological patterns of primary tumour and pulmonary metastases of malignant tumours in children and adolescents after complex therapy].

The histopathological features of the primary site and 102 pulmonal metastases were compared in the material from 40 malignancies in children and adolescents. All patients were treated by chemotherapy. Among the malignancies 30 were osteogenic sarcomas. A great histological variability in the multiple metastases removed at one surgical procedure and also in the metastases removed in the subsequent surgeries, were found. Summing up, the whole investigation; in 61.8% of metastatic focusses no difference was found in the histopathological patterns seen at the primary site and pulmonary metastases, in 17.6% total regression was observed, in 13.8% dedifferentiation and in 6.8% maturation of the tumours tissue were observed.

Adolescent↗
PubMedDate not supplied

[Correlation of the histological subtype of osteogenic sarcoma with the percentage of necrosis after initial chemotherapy].

The material comprises 195 cases of osteogenic sarcoma in children and adolescents. The tumours were grouped in accordance with two classifications; gradual classification which incorporates three levels of morphological maturation of sarcoma and structural classification which distinguishes conventional and nonconventional types of sarcoma. In every case histological mapping of the tumour was performed with the aim to establish the percentage of necrosis and postnecrotic resorbtion of the tumorous mass after the initial chemotherapy. The purpose of this study was to establish the correlation of the tumour necrosis with both histopathological classifications. The main conclusion was, that the gradual classification is more accurate for the changes occurring in the tumour tissue after chemotherapy.

Adolescent↗
PubMedDate not supplied

[Local recurrences after salvage operations in the therapy of osteogenic sarcoma cases - an analysis of adverse effects based on studied cases].

Therapeutic approach in osteogenic sarcoma until 1982 was connected mainly with surgery. Introduction of chemotherapy to the therapeutic protocols of osteogenic sarcoma improved the 5 years survival of patients with osteogenic sarcoma from 20% to 60-70%. The approach to surgical treatment was also changed. The principle of this treatment is usually to perform an operation to spare the limb with an intraoperative frozen section examination of bone marrow. In our Institute during the last 14 years about 300 cases were diagnosed, out of which about 200 were treated surgically, among these about 50% underwent treatment by salvage operations. Only in 7 cases local recurrences of disease were confirmed. Five of these cases were tumours of distal metaphysis of the femur, one case of proximal metaphysis of the tibia and one case of proximal metaphysis of the humeral bone. The sex range M/F was: 5/2, the average age of onset about 14 years. Various factors playing a role in therapeutic adversities were analysed. Among these were: radical surgery, grade of differentiation of the tumour, histological subtype, soft tissue infiltration, reaction to preoperative therapy and the type of chemotherapy. The conducted analysis indicates the role played by sparing surgery in adversities in therapy (3 cases). Attention was also given to the distinct tendency of osteogenic sarcoma to produce vascular embolism which is a source of haematogenously spreading metastases. In 7 cases vascular tumour embolism caused the appaerance of metastatic focuses adjacent to the primary tumour. A certain role in therapeutic adverities is played by the lack of response to preoperative chemotherapy (4/7 cases). The influence of other factors needs further investigation.

Adolescent↗
PubMedDate not supplied

[Aneuploidy and histopathological classification, according to the Musculoskeletal Tumour Society and WHO, of osteosarcoma in children and adolescents].

A flow cytometry analysis in children and adolescents with osteosarcoma was carried out. Investigation concerned 32 fresh surgical specimens (from patients after preoperative chemotherapy) and 18 paraffin-embedded tumour tissues (archive material). The correlation of aneuploidy population parameters to malignancy grading acc. to WHO (GI-GIII) and MSC histopathological classification, was investigated. It was shown that all specimens with aneuploid populations correlated with the histophatological diagnosis of osteosarcoma. Thus cytometry analysis can be a useful and rapid complementary method to conventional histophtatological diagnosis. On the contrary, no correlation was found between the aneuploid parameters and the low grade and high-grade osteosarcoma acc. to WHO and MSC classification. The Multicycle computer system is useful in retrospective analysis of paraffin-embedded tumour tissues. It is suggested that for this kind of investigation fresh diagnostic specimens (before preoperative chemotherapy) would be more adequate.

Adolescent↗
PubMedDate not supplied

[Clinical characteristics of osteosarcomas and cell aneuploidy in children and adolescents -- retrospective studies].

The relationship between the clinical status skeletal involvement, frequency of metastases and histopathological grading of osteosarcomas 18 children and adolescents and abnormal DNA content (aneuploidy) was analysed. Our findings demonstrate a correlation between Index DNA (DI), percentage of aneuploidy population to histopathological grading and prognosis. No significant correlation was found between aneuploidy and the site of skeletal involvement.

Adolescent↗
PubMedDate not supplied

[Flow cytometry estimate of s phase cell cycle and prognosis of osteosarcoma in children and adolescents].

Flow cytometry analysis has been used to measure the S phase in paraffin embedded osteosarcoma tissues of 22 children and adolescents who were surgically treated. The aim of this study was the assessment of S phase cell cycle in relation to survival, localization of tumour and histopathological grading. Our findings demonstrate the usefulness of flow cytometry determination of S phase cell cycle as a prognostic factor. Higher percentage of S phase cells predict worse prognosis.

Adolescent↗