Search PubMed⌕ Search

Biomedical subjects

E Marti

Publications and source records attributed to E Marti.

71 records · Page 4Linked to original sources
PubMed1991

Characterization of BPV-like DNA in equine sarcoids.

The DNA from equine sarcoid samples from New York State and Switzerland was isolated and probed with bovine papillomavirus type 1 (BPV-1) to determine if BPV genomes were present. Twelve of 13 sarcoids from New York State and 17/20 sarcoids from Switzerland contained DNA that hybridized to the BPV-1 probe. Restriction enzyme analysis of the positive samples demonstrated restriction fragment profiles characteristic of BPV-1 in 22 sarcoids and restriction fragment profiles characteristic of bovine papillomavirus type 2 (BPV-2) in 7 sarcoids. In addition, three tissues histologically diagnosed as pyogranulomatous dermatitis, fibropapilloma, and fibrosarcoma contained BPV-like DNA. Tissues with BPV-1-like and BPV-2-like DNA contained an average of 285.7 (21 to 808) and 125.8 (2 to 762) BPV-like genomes per cell, respectively. Minor differences in the restriction fragment profiles of the BPV-like DNA and evidence for partial BPV-like genomes were found in some sarcoids. BPV-like DNA was not detected in lymphocyte DNA from sarcoid-affected horses. These results confirm previous observations and support the hypothesis that bovine papillomavirus, or a very similar virus, is linked to the cause of equine sarcoid.

Animals↗
PubMed1991

The genetic basis of equine allergic diseases. 1. Chronic hypersensitivity bronchitis.

The genetic influence on chronic hypersensitivity bronchitis (CB) was investigated in families at two studs and among half-siblings of three affected and three non-affected sires at several farms. The family members at the two studs were born and raised under the same conditions, whereas the half-siblings were kept individually under very different conditions and were exposed to various environmental factors. The diagnosis was based on long-term observations and multiple clinical examinations at each of the two studs. In the half-sibling group, the diagnosis was based on the individual history and on a thorough clinical examination. The history of all horses suggested the disease was caused by allergies (symptoms provoked by hay). Statistical analysis of the data in the first study showed that a greater percentage of off-spring of two affected parents developed CB (9 of 13) than those with only one affected parent (23 of 48) and those with two healthy parents (5 of 29). The distributions of the affected offspring in these three categories (none, one or both parents affected) differed significantly (P less than 0.005) from what would have been expected without a genetic effect. The tendency to develop the disease was inherited equally from dams or sires. In the second stud fewer animals (n = 42) were included in the study, but the results were similar. Parents without a history of CB produced off-spring with a low incidence of disease (1 of 16) compared with a higher incidence among descendants of one or two affected parents (10 of 26; P = 0.02).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1991

Presence of substance P and angiotensin II in corticotropic cells of the lizard Gallotia galloti: immunochemical study in the adult and during ontogenesis.

The hypophysis of the lizard Gallotia galloti showed substance-P-like immunoreactivity in both the adenohypophysis (pars distalis, PD; pars intermedia, PI) and the neurohypophysis (median eminence and pars nervosa), whereas angiotensin-II-like immunoreactivity appeared only in PD and PI. The elution-restaining procedure has allowed us to demonstrate the colocalization of both peptides with adrenocorticotropic hormone (ACTH) in PD and PI cells. Electron microscopic study revealed the presence of substance P immunoreactivity on ACTH secretory granules. The ontogeny of both peptides in corticotropic cells has been studied, revealing that the presence of substance P in ACTH-containing cells of the PI occurs from the embryonic stage 33 (S 33), whereas in the PD it occurs from S 34, coinciding with the appearance of ACTH within the same cells. In both median eminence and pars nervosa of the neurohypophysis, substance P appeared later in development, at S 38. Angiotensin II immunoreactivity in PI cells first appeared at S 38, while in PD it appeared from S 40.

Adrenocorticotropic Hormone↗
PubMed1991

Ontogeny and immunohistochemical differentiation of the Pars Tuberalis in the lizard Gallotia gallotti.

The development of the pituitary Pars Tuberalis (PT) was studied in the lizard Gallotia galloti using classical, histological and immunohistochemical techniques. As early as stage 32 of development, the Rathke's pouch exhibits 2 lateral extensions that will give rise to the PT. In these extensions, cellular proliferation results in the formation of two cell masses which develop rostro-laterally and contact the basal diencephalon at stage 35. At stage 37, these two cell groups lose their connection with the pars distalis (PD). Until the end of embryonic development, connective tissue separates the nervous tissue from the glandular one. At hatching, the disappearance of this connective tissue results in the incorporation of the two cell groups into the nervous tissue without visible separation. During development, immunoreactivity for anti-betaLH and anti-TSH appears in the cells of the PT respectively at stage 39 and 37. In adults, almost all cells of the PT can be demonstrated using antisera against gonadotrophins.

Animals↗
PubMed1990

Early neuronal development in the spinal cord of a reptile assessed by neurofilament protein immunoreactivity.

The neural tube in the Gallotia galloti Stage 22 (S.22) embryo is already closed at the level of the cervical flexure, while caudal closure does not end until S.23. Cell proliferation begins shortly after the final neural tube closure, being rapid and giving rise, by S.26, to a thick ventricular zone. Cell migration does not start until S.28, initially in the basal plate, and forming putative motoneurons. Migration in the alar plate does not start until S.33. The appearance of the first neurofilament protein-positive perikarya coincides with the structural and ultrastructural identification of the neuron, although neurofilament-immunoreactive cells can already be identified inside the ventricular zone. Finally, from S.35 onwards, after major cell migration has already occurred, neuronal maturation continues in situ.

Animals↗
PubMed1990

Neuropeptide tyrosine (NPY) and its C-terminal flanking peptide (C-PON) in the developing and adult spinal cord of a reptile.

The developmental pattern and the adult distribution of neuropeptide tyrosine (NPY) and its C-terminal flanking peptide (C-PON) were investigated in the spinal cord of the lizard Gallotia galloti. Embryonic, postnatal and adult animals were studied by means of the immunohistochemical technique. Neurons containing both peptide immunoreactivities first appeared at the embryonic Stages 37/38. Immunoreactive perikarya were more numerous in the embryonic than in either the postnatal or the adult spinal cord. Fibres immunoreactive for both antisera appeared around the time of hatching, being numerous and widespread in the adult spinal cord. NPY- and C-PON-like-containing neurons and fibres in the adult spinal cord of Gallotia galloti were related to sensory, autonomic and motor areas.

Animals↗
PubMed1987

Ontogeny of peptide- and amine-containing neurones in motor, sensory, and autonomic regions of rat and human spinal cord, dorsal root ganglia, and rat skin.

The developmental patterns of neurofilament triplet proteins, peptide and amine immunoreactivities were compared in motor (ventral spinal cord), sensory (dorsal spinal cord, dorsal root ganglia, epidermis), and autonomic (intermediolateral cell columns, dermis) regions in the rat and human. In the rat, neurofilament triplet proteins first appeared in motoneurones (embryonic day 13). In the youngest human fetuses studied (6 weeks), immunoreactivity was present throughout the spinal cord. Peptides and amines occurred later. Calcitonin gene-related peptide, galanin, somatostatin, neuropeptide Y and its C-flanking peptide (CPON) were the first to appear localized to motoneurones (embryonic days 15-17 rat; fetal weeks 6-14 human). Numbers of immunoreactive motoneurones decreased toward birth, but immunoreactive fibers increased in the ventral horn with enkephalin, thyrotrophin-releasing hormone, and the monoaminergic markers 5-hydroxytryptamine and tyrosine hydroxylase (all presumably of supraspinal origin) the last to appear perinatally. In the dorsal horn, particularly in the rat, a transient expression of substance P-, somatostatin-, and neuropeptide Y/CPON-immunoreactive cells was detected (embryonic days 15-17). A pronounced increase of calcitonin gene-related peptide-, galanin-, somatostatin- and substance P- immunoreactive fibers was found perinatally in both species. This coincided with an increased detection of cells in the dorsal root ganglia containing these peptides and the earliest appearance of calcitonin gene-related peptide-, somatostatin-, and substance P-immunoreactive fibers in the rat epidermis. Few antigens were localized to the intermediolateral cell columns before embryonic day 20 (rat), fetal week 20 (human), with thyrotrophin-releasing hormone-, 5-hydroxytryptamine-, tyrosine hydroxylase-, and vasoactive intestinal polypeptide-immunoreactive nerves appearing perinatally. In the rat dermis, tyrosine hydroxylase-immunoreactive fibers (sympathetic fibers) and fibers immunoreactive for neuropeptide Y/CPON and vasoactive intestinal polypeptide were detected from postnatal day 1. In conclusion, 1) peptide and amine immunoreactivity develops in motor before sensory or autonomic regions, 2) many peptide-containing cells are transient in fetal life, and 3) central terminals of dorsal root ganglion cells express peptides before terminals in the skin.

Animals↗
PubMed1985

Circulating and brain metabolites of minaprine in the baboon.

A mixture of 15N-labelled, 14C-labelled and unlabelled minaprine was administered orally to three baboons, and metabolites in blood, urine and brain investigated. Biological samples were extracted with dichloromethane and the radioactive components extracted were analysed by t.l.c. and autoradiography. Compounds identified by comparing their physiochemical properties with those of synthetic standards and by g.l.c.-mass spectrometry were minaprine, 3-[2-(3-oxo)morpholino-ethylamino]-4-methyl-6-phenylpyridazine, 3-amino-4-methyl-6-phenylpyridazine, 3-[2-(aminoethyl)ethylamino]-4-methyl-6-phenylpyridazine, p-hydroxyminaprine and minaprine N-oxide. In addition to the urinary metabolites, two circulating metabolites were detected: metabolite A, 3-[2-(3-oxo)morpholino-ethylamino]-4-methyl-6-phenylpyridazine, and metabolite B (unidentified). All circulating metabolites appeared very early in blood, confirming the rapid and extensive metabolism of the drug. Metabolites A, B and 3 (p-hydroxyminaprine) were the major metabolites present in plasma. The parent drug was not the major circulating form, and was present in a higher concentration in erythrocytes than in plasma. Erythrocytes might act as a reservoir of the drug and could explain the relatively slow blood clearance of minaprine despite its rapid metabolism. The qualitative metabolic profile in brain tissue was similar to that in blood.

Animals↗
PubMedDate not supplied

Inbreeding, microsatellite heterozygosity, and morphological traits in Lipizzan horses.

While the negative effects of inbreeding and reduced heterozygosity on fecundity and survival are well established, only a few investigations have been carried out concerning their influence on morphological traits. This topic is of particular interest for a small and closed population such as the Lipizzan horse. Thus, 27 morphological traits were measured in 360 Lipizzan mares and were regressed on the individual inbreeding coefficients, as well as on the individual heterozygosity and mean squared distances (mean d(2)) between microsatellite alleles within an individual. Both individual heterozygosity and mean d(2) were based on 17 microsatellite loci dispersed over 14 chromosomes. The results obtained by multivariate analysis reveal significant effects of stud (P <.0001), age at measurement (P <.0001), and mean d(2) (P =.0143). In univariate analyses, significant associations were obtained between length of pastern-hindlimbs and inbreeding coefficient (P <.01), length of cannons-hindlimb and mean d(2) (P <.01), and length of neck and mean d(2) (P <.001). After adjustment of single-test P values for multiple tests (Hochberg's step-up Bonferroni method), only the association of the length of neck and mean d(2) remained significant (P =.0213). Thus, no overall large effects of inbreeding, microsatellite heterozygosity, and mean d(2) on morphological traits were observed in the Lipizzan horse.

Animals↗
PubMedDate not supplied

[The year 2002 national register on home-based parenteral nutrition].

AIM: To report on the results of the Registry on Home-based Parenteral Nutrition (HPN) of the NADYA-SENPE working group, corresponding to the year 2002. MATERIALS AND METHOD: Compilation of the registry data loaded by the Units in charge of HPN patients care. It consists of an on-line registry available to the registered users of the group's web page (www.nadya-senpe.com). Epidemiological, diagnostic, access route, complications, hospital admissions, degree of disability, and course until December 31st of 2002. RESULTS: Data from 74 patients were gathered (56.8% women and 43.2% men), from 18 hospital centers. Mean age of adult patients was 49.4 +/- 15.5 years and 2.3-1.1 years for patients younger than 14 years (n=3 patients). Diseases that prompted the use of HPN were mesenteric ischemia (29.7%), followed by neoplasms (16.2%), radiation enteritis (12.2%), motility impairments (8.1%), and Crohn's disease (5.4%). Tunneled catheters were used in 52.7% of cases, as compared to 36.5% of subcutaneous reservoirs. Mean treatment duration was 8.7 +/- 4.4 months; 68.9% of patients remained on HPN for a duration longer than 6 months, and in 41.9% longer than one year. Patients' follow-up was mainly done from the reference hospital (87.8%), and the remaining patients (12.5%) by the home care team. In no case patients were followed by the primary care team or other specialists than the ones that prescribed nutritional support. In 94 cases there were complications related to nutritional therapy. The more frequent complications presented were infectious. These complications represented 1.84 admissions per patient. The mean number of visits was 12.9 per patient (10.2 routinary visits and 2.7 emergency visits). At the end of the year, we observed that 74.3% patients stayed in the program, whereas in the remaining 23.6% HPN had been discontinued. The main causes for discontinuation were death (52.9%), and switch to oral diet (23.5%) or enteral nutrition (11.8%). With regards to disability degree, 16.1% were confined to a wheelchair or bed, and 17.6% had no disability at all or only a mild social disability. CONCLUSIONS: We observed a sustained HPN prevalence rate in Spain (1.8 patient pmp). The main cause for its use was short bowel syndrome secondary to vascular disease, followed by cancer. Complications associated to nutritional therapy were common, especially of infectious origin.

Adolescent↗
PubMedDate not supplied

[Jeune syndrome and fungal bronchial asthma].

A young girl suffering from asphyxiating thoracic dystrophy secondary to bronchial asthma was submitted to an allergologic evaluation. We have not found these processes associated in what some authors have called the "minor forms" of the Jeune syndrome. Osteochondrodysplasia, called in recent papers thoracic-pelvic-phalangeal dysplasia, is characterised by marked retraction of the thoracic cage associated with changes in the bones of the pelvis and extremities. We placed special emphasis on the classification of the different forms of presentation as being of greater or lesser severity from a clinical point of view, and stressed the importance of associated abnormalities (such as renal complications, as in the case of our patient) with respect to the eventual prognosis. The diagnosis of allergy is based on a detailed clinical history, positive results to cutaneous testing and the presence of specific IgE, together with the results of nasal provocation. The recording by rhinomanometre, of the temperature and pressure, and the assessment of the nasal mucosa following contact with the suspected allergen was also of help. Finally, having established the diagnosis, we advised the corresponding treatment, both from the allergic and renal viewpoints and concluded with an evaluation of the prognosis as conditioned by the renal pathology.

Asthma↗