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E Mansfield

Publications and source records attributed to E Mansfield.

34 records · Page 2Linked to original sources
PubMed1988

Educational priorities in mental health professions: do educators and consumers agree?

Empirical comparisons of priorities of consumers and educators in university-based programmes in psychiatry, psychiatric nursing and social work are described. Data resulted from a Q-sort of 200 objectives for mental health education. The results of the tests of validity and reliability of the instrument are described, followed by a discussion of the similarities and differences among the four samples of subjects: consumers and educators in the three mental health professions. Suggestions for applications of this methodology by inter- and intradisciplinary service providers are described.

Attitude of Health Personnel↗
PubMed1984

Rapid searches for complex patterns in biological molecules.

The intrinsic redundancy of genetic information makes searching for patterns in biological sequences a difficult task. We have designed an interactive self-documenting computer program called QUEST that allows rapid searching of large DNA and protein data banks for highly redundant consensus sequences or character patterns. QUEST uses a concise language for specifying character patterns containing several levels of ambiguity and pattern arrangement. Examples of the use of this program for sequence data are given. Details of the algorithm and pattern optimization are explained.

Amino Acid Sequence↗
PubMed1982

Comparison of psychiatric-mental health nursing education objectives: consumers, educators, and practitioners.

This consumer-nurse study was part of a larger project, Research in Mental Health Education, which compared the priorities of educators and practitioners within and between three core mental health professions and between the professionals and consumers. In the consumer-nurse portion of the study, the objectives for graduate education in psychiatric-mental health nursing were validated by psychiatric-mental health nursing educators (N=20) based in four university programs in the East, West, and Midwest. Another sample of nurse educators and practitioners in this specialty area, drawn from nine states, prioritized these objectives. A separate deck of objectives for mental health professionals was generated and prioritized by consumers (N=15) who were members of the Mental Health Association of Minnesota. Standardized procedures for generating and validating the objectives for nurses and consumers and the same task, the Priority Sort, were used by all subjects, nurses, and consumers, to prioritize the objectives. Similarities and differences between nurses and consumers are discussed. Differences between nurse educators and practitioners, which are statistically significant, also are described. Implications for consumer and practitioner input into curricular decisions are discussed as well as interdisciplinary courses that could conserve faculty time and budgetary resources.

Community Participation↗
PubMed1980

A conceptual framework for psychiatric-mental health nursing.

A conceptual framework for a psychiatric-mental health nursing program gives direction for course content and becomes a rich source of hypotheses leading to development of theory. For this described framework tenets of humanistic theorists provide the philosophical base: Belief in the individual's responsibility, capacity and potential for growth; meaning of life; respect and dignity of the individual; and freedom of the individual for self-determination. Four major components comprise the framework: social systems and developmental theories, communication concepts, and self-esteem. Set in the nursing process schema, concepts from social systems and developmental theories are used in the assessment, concepts from communication theories are the basis for intervention, and changes in self-esteem become a measure of evaluation. The framework is applicable for individual clients, families, and/or groups. The nurse therapist is viewed in the same framework which may evolve into an intersystem model. Numerous research questions are suggested which may validate the relationships between the components of the framework.

Communication↗
PubMedDate not supplied

Characterization of RFB4-Pseudomonas exotoxin A immunotoxins targeted to CD22 on B-cell malignancies.

To develop an immunotoxin for the treatment of B-cell malignancies, we constructed several candidate conjugates with RFB4, a B-cell specific anti-CD22 IgG1, and truncated forms of Pseudomonas exotoxin (PE). The four versions of PE included PE35 and PE35KDEL, which were linked to RFB4 via a disulfide bond, and PE38 and PE38KDEL, which were linked via a thioether bond. The PE35 truncated forms, which are fully active in ADP ribosylation and lack receptor binding sequences, do not require intracellular proteolytic cleavage in order to be active. PE35KDEL has the consensus endoplasmic reticulum retention signal, KDEL, replacing the wild type PE C-terminal sequence, REDLK. The PE38 forms retain all of domain II and therefore require cleavage to be active within cells. Cytotoxicity experiments on CD22-positive cell lines revealed that the PE35 conjugates were more active than the PE38 versions and the presence of the KDEL sequence generally enhanced toxicity by 5-10-fold compared to that of REDLK. The RFB4-PE35KDEL immunotoxin was most active in cytotoxicity assays against Burkitt's lymphoma cell lines such as Daudi and CA46 (IC50 = 0.2 ng/mL) and displayed little cytotoxicity toward human vascular endothelial cells (IC50 > 20 micrograms/mL). Results of experiments conducted in nude mice showed that both RFB4-PE35KDEL and RFB4-PE35 could inhibit the development of subcutaneous CA46 tumors.

ADP Ribose Transferases↗
PubMedDate not supplied

Parallel molecular genetic analysis.

We describe recent progress in parallel molecular genetic analyses using DNA microarrays, gel-based systems, and capillary electrophoresis and utilization of these approaches in a variety of molecular biology assays. These applications include use of polymorphic markers for mapping of genes and disease-associated loci and carrier detection for genetic diseases. Application of these technologies in molecular diagnostics as well as fluorescent technologies in DNA analysis using immobilized oligonucleotide arrays on silicon or glass microchips are discussed. The array-based assays include sequencing by hybridization, cDNA expression profiling, comparative genome hybridization and genetic linkage analysis. Developments in non microarray-based, parallel analyses of mutations and gene expression profiles are reviewed. The promise of and recent progress in capillary array electrophoresis for parallel DNA sequence analysis and genotyping is summarized. Finally, a framework for decision making in selecting available technology options for specific molecular genetic analyses is presented.

DNA↗