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Biomedical subjects

E Madden

Publications and source records attributed to E Madden.

6 recordsLinked to original sources
PubMed2000

Accelerated prediction of recombinant protein production in Saccharomyces cerevisiae by using rapid monitoring techniques.

The use of a stopped-flow analyser for the monitoring of the production of a secreted recombinant protein, a wild-type cutinase, from S. cerevisiae CEN.PK111-32D pUR7320 is described. Induction is through use of a galactose promoter, and the monitoring facility is used to record the formation of the cutinase and cell density with optical density measurements. A range of induction conditions was studied with a view to using the monitoring to predict the likely level of cutinase formation. Results achieved within 4 to 5 h of induction were of sufficient quality to allow the use of simple modelling relating cutinase formation and cell production to predict likely final specific activities of the product. The utility of such monitoring and prediction is discussed with regards to improved process confidence and definition during fermentation production.

Journal Article↗
PubMed1999

Crystal morphology prediction and morphology variation in NaIO4 and NaIO4.3H2O.

Attachment energies computed with only Coulomb potentials have been utilized to predict crystal morphologies for NaIO(4), sodium iodate, and the hydrate NaIO(4).3H(2)O, sodium iodate trihydrate [actually Na(H(3)O)(IO(3))(OH)(3)]. As with other previously studied water-soluble ionic compounds, these two systems exhibit a systematic relationship between the early growth morphology and that of mature crystals; this relationship can in each case be reproduced by adjusting one attachment energy value. Morphology prediction for these two substances is of particular interest because NaIO(4).3H(2)O is a polar crystal and involves extensive hydrogen bonding, and because obtaining the observed morphology for NaIO(4) involved consideration of solvent desorption at the growing faces.

Journal Article↗
PubMed1997

The well of loneliness, or the gospel according to Radclyffe Hall.

Radclyffe Hall's 1928 novel, The Well of Loneliness, is repeatedly described as a "bible" of lesbian literature. The novel itself repeatedly alludes to biblical stories, especially the story of Christ. Yet there has been little sustained analysis of the biblical language of the novel. Most feminist and lesbian critics have dismissed the biblical allusions and language as unfortunate and politically regressive; religious critics have ignored the novel. This essay reexamines the biblical nature of the novel, especially its portrayal of the lesbian Stephen Gordon as a Christ figure. The study further claims a creative and interventionary power in Hall's use of biblical narratives and tropes, a power traceable in public reception to the novel and in courtroom reactions to the use of spiritual language in a text about lesbianism. By writing the life of a lesbian as a kind of gospel of inversion, Hall turns a language of condemnation into a language of validation, making her use of biblical language a kind of Foucauldian "reverse discourse." The novel's power lies in its portrayal of a lesbian messiah, and in its joining of sexological and religious discourses.

Bible↗