Search PubMed⌕ Search

Biomedical subjects

E M Messing

Publications and source records attributed to E M Messing.

At least 73 records · Page 4Linked to original sources
PubMed1987

Method for assuring accuracy of bone biopsy using technetium 99 bone scan.

In the clinical staging of malignancy, radionuclide bone scanning has played an increasingly important role. An area of increased radionuclide uptake on technetium 99 (99Tc) bone scan which is not visualized on skeletal radiographs can present a significant diagnostic dilemma. This can be further compounded by nonrevealing percutaneous or open surgical biopsies. The authors present a method of definitively localizing the appropriate site for bone biopsy in such circumstances. Use of this technique has allowed us to ascertain that isolated rib lesions in two patients with extra-osseous malignancies were not due to metastatic disease.

Aged↗
PubMed1987

Epidermal growth factor--interactions with normal and malignant urothelium: in vivo and in situ studies.

Epidermal growth factor (EGF) is excreted in urine in high concentrations and thus incubates with bladder epithelial cells continuously. However, it is not known whether any urothelial cells can bind urinary EGF or respond to it. Using a monoclonal antibody (528) to the binding portion of the human EGF receptor, immunoperoxidase staining demonstrated that the basal cell layer of normal urothelium is richly endowed with cell surface EGF receptors while the superficial cell layer is not. Alternatively, superficial cells of premalignant and malignant urothelium have many surface EGF receptors. Intravesical EGF induces in vivo activity of ornithine decarboxylase and DNA synthesis in rat bladders, with nuclear thymidine incorporation being limited to the basal epithelial cell layer. These studies indicate that urothelium can respond to urinary EGF and that this response parallels the distribution of EGF receptors. These findings combined with the difference in EGF-receptor expression between malignant and normal cells indicate that urinary EGF may play a role in bladder tumor development and/or growth.

Animals↗
PubMed1987

The significance of asymptomatic microhematuria in men 50 or more years old: findings of a home screening study using urinary dipsticks.

In an ongoing home screening study 231 men 50 or more years old without known causes of hematuria have tested their urine each week with a chemical reagent strip for the presence of blood. After 3 months of testing 23 patients have had hematuria at least once. Of these men 5 have had urinary cancers and 5 have had other serious underlying diseases requiring immediate treatment. In only 3 of these 10 men (only 1 with cancer) did hematuria occur in more than a third of the testings or on subsequent microscopic urinalysis. The degree of hematuria was unrelated to the seriousness of its cause. We conclude that in this population hematuria occurs so intermittently that when found on routine urinalysis, regardless of quantity, serious underlying pathological conditions must be ruled out aggressively. Furthermore, regular hematuria home testing appears to offer promise as an economical means to detect urinary cancers and other serious diseases in asymptomatic men 50 or more years old.

Hematuria↗
PubMed1987

The diagnosis of interstitial cystitis.

The diagnosis of interstitial cystitis can be firmly established by evaluating symptoms and history carefully, ruling out other diseases which can mimic its clinical picture, and performing the necessary cystoscopic examination (almost always under anesthesia). We also advocate that biopsy be performed in all individuals; although, since no pathognomonic abnormalities are demonstrable, it is unclear if the diagnosis of interstitial cystitis can be established or excluded by biopsy alone (unless, of course, another disease is encountered). While urodynamic studies are primarily useful to rule out interstitial cystitis, they may, in addition, have a role in subsequent management and/or in assessment of therapeutic response and thus, at least simple cystometry should probably also be performed. However, more complex urodynamic (e.g., electromyography, urethral pressure profilometry, etc.), radiographic (e.g., intravenous urography, voiding cystourethrography, or computerized axial tomography), laboratory or surgical (e.g., laparoscopy, prostate biopsy) studies are of minimal value in diagnosing interstitial cystitis and should only be done in the face of clinical evidence indicating their appropriateness.

Biopsy↗
PubMed1986

Lymphocele after retroperitoneal node dissection for testis tumor.

A patient is presented in whom a lymphocele developed after a retroperitoneal lymph node dissection for Stage II embryonal carcinoma of the testicle. The benign nature of this lymphocele has been confirmed not only by the diagnostic procedures outlined, but by its stability over a 42-month follow-up period with no further antitumor therapy. We conclude from reviewing the literature that while aggressive measures are necessary to confirm the diagnosis of a lymphocele, its management should be expectant. If significant obstruction of neighboring structures occurs, an attempt at percutaneous drainage (and possibly sclerosis) seems appropriate despite potential risks of interventional treatment such as hemorrhage and introduction of infection. Open procedures for marsupialization and drainage should be reserved for cases in which more conservative measures fail.

Adult↗
PubMed1986

Nonseminomatous germ cell tumor of the testicle: does extensive staging of the primary tumor predict the likelihood of metastatic disease?

To assess the hypothesis that local extent of the primary lesion in patients with nonseminomatous germ cell tumors of the testicle can predict disseminated disease, clinicopathological correlations of the primary tumor and metastases were determined in a retrospective review of 120 patients treated at our institution from 1970 to 1982. Pathological staging was available for all 93 patients with subdiaphragmatic disease and the primary tumor was examined by routine histological techniques in all cases. Increased primary tumor stage, as evidenced by invasion into the tunica vasculosa or extension into the rete testis, epididymis and/or lower or upper spermatic cord, was associated with metastatic disease in 91, 96, 97 and 97 per cent of the patients, respectively. Only 9 per cent of the patients with pathological stage A disease had vascular invasion compared to 45 and 67 per cent of those with stages B and C disease, respectively (p less than 0.01). Furthermore, metastases and/or eventual dissemination occurred in 84 per cent of the patients with clinical stage A disease and vascular invasion, and in only 23 per cent of those without vascular invasion (p less than 0.01). Size of the primary tumor was not of predictive value. Local extension of the primary lesion was common with embryonal carcinoma but it was not demonstrated when the predominant histological type was teratoma or teratocarcinoma, although metastases were present in 37 and 46 per cent of the latter cases, respectively. The implications of these findings, especially with regard to expectant management for clinical stage A nonseminomatous germ cell testis tumors, are discussed.

Adolescent↗
PubMed1985

Bilateral rectus femoris pedicle flaps for detrusor augmentation in the prune belly syndrome.

A 20-year-old patient with the prune belly syndrome presented with urinary retention and renal failure. In preparation for renal transplantation bladder rehabilitation, consisting of urethral dilation, reduction cystoplasty and detrusor augmentation with paired rectus femoris pedicle flaps, was undertaken. This procedure enabled the patient to empty the bladder totally by spontaneous voiding. Successful renal transplantation was performed subsequently and he has not required intermittent or continuous catheterization. The surgical approach and technique are described, and their application to other conditions of detrusor hypotonicity is discussed.

Abdominal Muscles↗
PubMed1985

Microscopically controlled surgery in the treatment of carcinoma of the penis.

Complete removal of carcinoma of the penis can be achieved by excision of the neoplasm layer by layer and microscopic examination of the entire undersurface of each layer by the systematic use of frozen sections. Microscopic guidance provides substantial assurance of eradication of the primary cancer, and permits preservation of maximal amounts of normal tissue and normal functions. In a series of 29 consecutive cases of squamous cell carcinoma of the penis the 5-year cure rate among the 25 determinate cases was 68 per cent. The fact that in this series only 1 patient had cancer limited to the prepuce is evidence that circumcision usually is successful for neoplasms so limited. The primary carcinoma was eradicated in 23 of the 25 lesions (92 per cent).

Aged↗
PubMed1984

Murine hybridoma antibodies against human transitional carcinoma-associated antigens.

Spleen cells from mice immunized with the human urinary bladder transitional cell carcinoma cell line 647V have been fused with a syngeneic myeloma cell line to produce hybridomas. Screening of supernatants from 40 hybridomas which reacted with the immunizing cell line identified antibodies recognizing a variety of common, shared and tumor-associated antigens as well as newborn calf serum dependent antigens. Three hybridoma antibodies, 9A7 , 2E1 and 2A6 , recognize antigens found on all the human transitional cell carcinoma cell lines and tissue preparations tested, but the antigens were not found on normal human tissue (including urothelium), thus demonstrating the capability of the antibodies to distinguish normal from malignant bladder transitional epithelium. These antibodies, however, otherwise differ in their patterns of reactivity, with 1 recognizing an antigen which is also expressed on highly anaplastic malignant non-transitional cell carcinoma cell lines and tumors, while the other 2 demonstrate reactivities which are far more restricted to transitional cell carcinoma.

Animals↗
PubMed1984

Growth stimulating activity produced by human bladder cancer cells.

Growth promoting properties have been identified in the conditioned serum-free medium from cultures of the human transitional cell carcinoma cell line 647V. This activity appears to reside in a molecule of more than 5000 MW. The factor responsible for 647V growth is distinguished from epidermal growth factor, since it fails to inhibit the specific binding of epidermal growth factor by 647V cells, and 647V cells are not stimulated to grow by epidermal growth factor. It also does not appear to be an insulin-like growth factor-like molecule since it fails to competitively bind with human insulin-like growth factor-carrier protein.

Binding, Competitive↗
PubMed1984

Cultures of normal human urothelial cells from ureters of perfused cadaver transplant kidneys.

In this comparative study, we report that urothelial cultures initiated from ureters of mechanically perfused cadaver-obtained transplant kidneys display characteristics of explant attachment, explant viability, cellular morphology and area of outgrowth essentially indistinguishable from cultures established from ureters of living donor kidneys. We conclude that ureter specimens from cadaver kidneys are a suitable, routinely available source of tissue from which cultures of normal human urothelial cells can be propagated.

Cadaver↗
PubMed1982

Serum-free medium for the in vitro growth of normal and malignant urinary bladder epithelial cells.

A serum-free medium, DH-S1, is described which is valuable for the establishment of primary cultures of normal and malignant transitional epithelium (transitional cell carcinoma of the bladder). Growth of epithelial cells in DH-S1 is facilitated but that of fibroblasts is suppressed. Another established human transitional cell carcinoma cell line, 647V, has grown continually in DH-S1 for over 36 passages. Morphological and antigenic studies comparing 647V cells growing in serum-containing and serum-free medium reveal differences which are pronounced at low cell density but which almost disappear at higher densities. A new human transitional cell carcinoma cell line, LA-B1, is described whose initial growth was supported by this serum-free medium.

Animals↗
PubMed1979

Complication of Clorpactin WCS90 therapy for interstitial cystitis.

A case of ureteral fibrosis complicating Clorpactin WCS90 treatment for interstitial cystitis in a patient with vesicoureteral reflux is presented. The results of a laboratory experiment designed to study the effects of Clorpactin WCS90 on refluxing ureters are discussed.

Animals↗
PubMed1979

Stones in orthotopic, non-obstructing ureteroceles.

A case is presented in which stones were found bilaterally in orthotopic, non-obstructing ureteroceles in a child. This is apparently the first reported instance of this condition occurring in the presence of sterile urine. A review of related literature gives clues to the possible etiology of such an occurrence.

Child, Preschool↗
PubMed1978

Interstitial cystitis: early diagnosis, pathology, and treatment.

In a retrospective review, 52 patients with interstitial cystitis have been studied. Patients with persistent lower tract irritative symptoms, repeatedly sterile urine, and negative urine cytology must be suspected of having interstitial cystitis, and a diagnosis of urethral syndrome in such patients is highly questionable until cystoscopy under anesthesia has been performed. We believe that the finding of multiple petechia-like hemorrhages (glomerulations) on the second distention of the bladder is the hallmark of interstitial cystitis, and that a reduced bladder capacity and a Hunner's ulcer represent a different (classic) stage of this disease. In all stages, the characteristic histologic finidng is submucosal edema and vasodilation. The presence of eosinophils and mast cells is variable, and even in the classic disease the muscularis often appears to be normal. Immuno fluorescent studies and laboratory tests, including the fluorescent antinuclear antibody test (FANA), have not helped us to diagnose (or investigate) interstitial cystitis. Bladder instillations with a 0.4 per cent solution of oxychlorosene sodium (Clorpactin WCS-90) have provided remarkable relief for many patients with this disease, particulary those with the classic form.

Adult↗