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Biomedical subjects

E M Mackay-Scollay

Publications and source records attributed to E M Mackay-Scollay.

18 recordsLinked to original sources
PubMed1979

Single-radial-hemolysis test for the assay of rubella antibody in antenatal, vaccinated, and rubella virus-infected patients.

The single-radial-hemolysis test (SRHT) is a simple, convenient, and sensitive method that provides accurate measurement of IgG antibody to rubella virus. Lipoprotein inhibitors of rubella hemagglutination do not interfere with the SRHT; thus the assay of low levels of antibody is more reliable by SRHT than by the rubella hemagglutination-inhibition test (RHIT). The technique for the SRHT in a form suitable for large numbers of tests is detailed and compared with that of the RHIT, and the reproducibility of the SRHT in routine operation is assessed. Results obtained by testing various populations in Western Australia are used to show the application of the SRHT in the diagnosis of rubella and in the monitoring of rubella vaccination programs.

Adolescent↗
PubMed1978

A survey of rotavirus associated with gastroenteritis in Aboriginal children in Western Australia.

In a year-long survey which was carried out between April, 1975, and March, 1976, faecal specimens from Aboriginal and some non-Aboriginal children who suffered from gastroenteritis were examined by electron microscopy for rotavirus, and also tested for bacteria and parasites. The children were under six years of age and came from all parts of Western Australia, except Perth. Rotaviruses, "astroviruses" and adenoviruses were detected as well as the usual potentially pathogenic bacteria and parasites. Two peaks of rotavirus incidence were found during the year in specimens from Kalgoorlie, but only one peak occurred in Derby. Rotavirus was somewhat more frequently seen in non-Aboriginal than Aboriginal children.

Age Factors↗
PubMed1978

Coronavirus-like particles in Aboriginals and non-Aboriginals in Western Australia.

Pleomorphic virus-like particles which resemble coronaviruses in morphology were found in faecal specimens from Aboriginal and non-Aboriginal children in Western Australia. They were equally prevalent in those with or without symptoms of diarrhoea. A much greater proportion of Aboriginal than non-Aboriginal children were found to be excreting the particles. The proportion of children who excreted the virus-like particles increased with age. Coronavirus-like particles, indistinguishable from those found in humans, were found in a number of Aboriginals' dogs.

Adult↗
PubMed1975

Acute transmural myocardial infarction associated with active Coxsackie virus B infection.

Out of a total of 233 patients suffering from transmural myocardial infarction, 20 patients were found to have serologic evidence of a concurrent active Coxsackie virus B infection. While the infection may have been coincidental, it is also possible that the virus may have played some part in the illness. Conceivably, myocarditis could be mistaken for infarction or, by some mechanism as yet unknown, the virus might precipitate infarction in susceptible subjects.

Acute Disease↗
PubMed1975

Epidemiological studies of Epstein-Barr herpesvirus infection in Western Australia.

In a study of a Caucasian population in Western Australia the prevalence of antibodies to Epstein-Barr virus (EBV) was 41% in the 9- to 10-year age group, 80% in the 16 to 19-year age group and 92% in young adults. The age-specific annual seroconversion rates indicated two peaks of primary EBV infection in the population studied - one under 5 years of age and the other at adolescence. The geometric mean titre rose with age, from 23 at 5-6 years to 53 at 36-40 years. It was shown that in 73 families studied there was evidence of probable spread of EBV infection among siblings, particularly between those of the same sex. Serological study of patients with infectious mononucleosis indicated that 100% of those examined had antibody to EBV and the geometric mean titre was elevated to 210. Rising titres and seroconversion was demonstrated in these patients together with successful establishment of EBV-carrying cell lines from the peripheral blood in two-thirds of the cases.

Adolescent↗
PubMed1974

A simple method for detecting mycoplasma infection of cell cultures.

This paper describes the overnight Giemsa staining method for detecting mycoplasma infection of cell cultures. This simple method requires no special equipment and has practical application in most cell culture laboratories. This technique has been used conveniently to monitor stock cell cultures for mycoplasma infection over a 12-month period avoiding any contamination.

Amnion↗
PubMed1972

An evaluation of strontium chloride, Rappaport and strontium selenite enrichment for the isolation of salmonellas from man, animals, meat products and abattoir effluents.

Strontium chloride enrichment broth was found to be comparable to Rappaport broth for the recovery of a wide range of Salmonella serotypes from man, animals, meat products and effluents. With the exception of cloacal samples from reptiles, both procedures were superior to selenite F.The performance of strontium chloride Mand selenite F enrichment was improved when effluent samples were incubated at 43 degrees C.Strontium chloride M and Rappaport enrichment were superior to selenite F for the isolation of Arizona species from reptiles.Strontium chloride B, strontium selenite and Rappaport broths were found suitable for the isolation of multiple Salmonella serotypes from sea water contaminated with abattoir effluents. The strontium chloride B and strontium selenite enrichment media were superior to Rappaport broth when samples were incubated at 43 degrees C.Modified bismuth sulphite agar was found superior to Salmonella-Shigella agar as a solid subculture medium.The investigation of a food poisoning outbreak due to Salmonella typhimurium phage type 21 is reported.The significance of the choice of sampling and isolation techniques in salmonellosis in man and animals is discussed.

Abattoirs↗
PubMed1969

A simple quantitative and qualitative microbiological screening test for bacteriuria.

A simple, accurate screening test for significant bacteriuria is described. It entails brief contact of a freshly voided specimen of urine with the surface of a solid culture medium lining the wall of a transparent disposable plastic container, incubating it at 37 degrees C for 24 hours, and counting colonies developing on a proscribed surface area. The method obviates errors due to contaminants, affords a limited species differentiation of the causative organisms, and provides a ready means of obtaining a direct sensitivity test on the responsible bacteria.

Anti-Bacterial Agents↗