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Biomedical subjects

E M Lance

Publications and source records attributed to E M Lance.

At least 73 records · Page 4Linked to original sources
PubMed1969

The mechanism of action of anti-lymphocyte serum. Studies of antibody eluate.

Studies designed to gain insight into the mechanism of action of the active component of antilymphocyte serum were carried out using an antibody eluate prepared from the IgG fraction of anti-lymphocyte serum by absorption and subsequent elution from thymocyte membrane. The resulting antibody eluate was labeled with radionuclide tracer to determine the fate of the antibody in vivo. The result indicated that anti-lymphocytic antibodies are eliminated from recipients extremely rapidly. The mechanism for this rapid clearance appears to depend upon the absorption of antibody molecules onto lymphocyte surfaces and the subsequent clearing and degradation of the antibody-lymphocyte complexes by the reticuloendothelial system. Distribution studies confirm that the major site of antibody-lymphocyte interaction occurs in the periphery with relatively little penetration of antibody within lymphoid organs. Radioautographic studies showed that the pattern of localization within lymphoid and other organs is confined to rather specific areas. These observations are believed to offer strong support for the notion that anti-lymphocyte serum achieves its immunosuppressive effect by bringing about a selective ablation of the population of recirculating lymphocytes.

Animals↗
PubMed1969

Tolerance of rat skin grafts in adult mice.

Adult CBA mice may be made tolerant of August rat tail skin grafts by short intensive treatments with antilymphocyte serum followed by massive ( approximately 100 x 10(6) cells) injections of August rat lymphoid cells. Prolonged tolerance can be achieved only in thymectomized mice, and is accompanied by lymphoid cell chimerism and the manufacture of rat protein. The rat lymphoid cell donors must be treated beforehand with antirat ALS to avoid complications associated with graft versus host reactions. Rejection of rat skin grafts is accompanied by the formation of high titres of antirat hemolysins and lymphocytotoxins, but in "tolerant" mice the rat skin survives in the continual presence of antirat antibodies. Under certain circumstances, however, passive transfusion of high titre mouse antirat serum can precipitate the breakdown of rat skin grafts. It is doubtful if this represents the primary mechanism of rejection. The reaction of normal mice against rat skin is essentially an intensified allograft reaction; it is at all events wholly immunological in character.

Animals↗
PubMed1968

Histopathological effects in mice of heterologous antilymphocyte serum.

The effects of heterologous rabbit anti-mouse lymphocyte antiserum on the morphology of lymphoid and other tissues was investigated in CBA mice. The lymphoid tissues exhibited characteristic changes specific for ALS treatment, which were an invariable accompaniment to its immunosuppressive effects. These consisted of peripheral lymphopenia occurring at some time during a course of ALS treatment and persistent depletion of small lymphocytes in lymph node paracortical areas and splenic follicular periarteriolar zones. The thymic histology was generally well preserved. It is suggested that the relevant lesions reflect a rapid depletion of the pool of recirculating lymphocytes, possibly by a primary cytotoxic effect exerted on cells peripheral to lymphoid tissue. Other histologic features attendant to the administration of ALS were accounted for as consequences of immunization of ALS recipients to rabbit serum constituents or by the deleterious effects of antibodies directed against tissues other than lymphoid cells.

Animals↗