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Biomedical subjects

E Leszczyńska

Publications and source records attributed to E Leszczyńska.

5 recordsLinked to original sources

Enzymatically modified ion-selective electrodes for flow injection analysis.

The application of potentiometric biosensors based on membrane ion-selective electrodes for flow injection analysis (FIA) is presented. The biosensors have been obtained by covalent binding of enzyme molecules directly to surface of ion-selective membranes. The biosensor/FIA systems enable the determination of urea and penicillin in the millimolar ranges of concentration. About 30 samples per hour can be analysed in the performed FIA systems. The operational stability of the bioanalytical systems exceeds 1 month.

Biosensing Techniques↗

[The pulmonary artery size variability during various stages of post-fetal life in man].

Fresh lungs (45 right and 40 left) from dead bodies of 50 (30 male and 20 female) people from 1 to 80 years were examined. The pulmonary arteries were injected for 65% duracryl solution (Spofa-Dental firm) and after maceration the preparations were measured. Pulmonary arteries indices were also calculated. Pulmonary arteries diameter in male measured from 9.0 to 24 mm, average 17.0 mm, in female--from 7.4 to 28 mm, average 18.7 mm. The difference between the right and left pulmonary artery size was statistically significant, both in male and female persons. The correlation indices between size of male right pulmonary artery and age, length and body weight amounted to 0.70, 0.61 and 0.53, while for left pulmonary artery--0.58, 0.55 and 0.49. The same indices of female specimens valued for right pulmonary artery 0.72, 0.58 and 0.51. The size of the left pulmonary artery was correlated only with age (index 0.65).

Adolescent↗

[Effect of cadmium on serum level of total protein and glycoprotein carbohydrate components in rats].

In experimental cadmium intoxication 100 rats were intraperitoneally injected with single doses of 0; 5 1,0, 2,0 5,0 mg Cd++/kg body weight as well as with 14 doses of 0,25 mg Cd++/kg. In the serum of animals the level of total protein and of glycoprotein carbohydrate components was determined. After doses in the range of cadmium concentration from 0,5 to 2,0 mg Cd++/kg body weight, a statistically and significant increase of the serum level of the protein-bound hexose hexoamine and sialic acids was stated. On the other hand the dose of 5,0 mg Cd++/kg caused only the increase of hexose in comparison with values of these constituents recorded in the control group. In animals receiving multiple doses of cadmium and also in those after a single administration of 1,0 mg Cd++/kg, a significant increase of the total protein level was started. The last group revealed the highest concentrations of protein and glycoprotein carbohydrate constituents on the third day after injection of cadmium while after 14 days, amouunts of these compounds approximated the levels estimated in the control group. A univocal evaluation of the obtained results is difficult, however in the mechanism of the quantitative alterations within the glycoprotein components of rats serum, the possibility of cadmium--induced biosynthesis of these compounds should be taken into consideration.

Animals↗