Search PubMed⌕ Search

Biomedical subjects

E Leonardo

Publications and source records attributed to E Leonardo.

At least 55 records · Page 3Linked to original sources
PubMed1985

[Immuno-oncologic monitoring of patients with bronchial carcinoma. II. Plasma fibronectin determination].

Recent attempts implicating glycoproteins have been focused on the study of fibronectin. This glycoprotein was observed especially in cellular adhesivity processes. Some works were direct to detect the fibronectin concentration in plasma and in other biological fluids to relate increased or decreased levels in pathological features. In this paper we describe a study on plasma fibronectin in lung cancer patients during the course of the disease.

Fibronectins↗
PubMed1985

[Immuno-oncologic monitoring of patients with bronchial carcinoma. I. Carcinoembryonic antigen (CEA)].

Plasma levels of carcinoembryonic antigen (CEA) were determined in 82 lung cancer patients before surgery and during the clinical course of the disease. Results were compared to those obtained in normal controls and in patients with lung inflammatory diseases. Furthermore, relationships between plasma CEA levels and survival were carried out. While CEA levels were not useful to make differential diagnosis, in our study, we hypothized the possibility to use CEA in prognostic evaluations.

Bronchial Diseases↗
PubMed1982

Monoclonal immunoproliferative disorder of B-lymphocytes IgD/lambda changing from chronic lymphocytic leukaemia to myeloma and plasmacytic leukaemia pattern.

An immunoproliferative disorder with M component IgD/lambda and a strong decrease of the other Ig classes in the serum is reported. The peculiarity of this disorder consists in the shift from a predominant chronic lymphocytic leukaemic pattern, at the beginning, to a true myeloma with plasmacytic leukaemia IgD/lambda several months later. The possibility of the removal, in this case, of the initial precocious block with derepression or 'switch on' in transformation of the malignant lymphocyte clone and, consequently, change of a type of malignant lymphoma into another more evoluted and more malignant type is discussed.

B-Lymphocytes↗
PubMed1980

[Evaluation of leukocyte migration inhibition (LIF) in patients with carcinoma of the lung before and after the use of levamisole in vitro].

Aspecific immunosuppression in neoplasia has long been known, even though all its biological aspects are not yet fully understood. Inhibition of leukocyte migration (LIF) was studied before and after the use of levamisole in vitro to determine whether changes occurred in cell reactivity. The results of the investigation are discussed in the light of modern immunopathological theories.

Humans↗
PubMed1980

[Solitary plasmocytoma of the lung. Anatomo-clinical considerations on a case].

A case of solitary plasmacytoma of the lung is presented. The patient is still alive three years after surgery. The anatomical and clinical features of this rare form are discussed. Stress is laid on the fact that diagnostic certainty can only be obtained immunopathologically. It is also recommended that patients be subjected to radiological and laboratory examination in the postoperative period, and every six months for several years, to detect the possible occurrence of multiple myeloma.

Aged↗
PubMed1980

G1 heavy chain disease: clinicopathological, ultrastructural and immunochemical study of a new case.

Clinicopathological ultrastructural, and immunohistochemical findings of a new case of IgG1 heavy chain disease are reported in detail. The abnormal protein lacks the VH and CH1 region with sequence starting at 225 residue. The main pathologic feature was plasmacytic tumor of the lymph nodes with B-cell immunoblastic sarcoma patterns. Neoplastic diffusion to other organs was also present. Plasmacytic neoplastic cells have also been found in the bone marrow. The ultrastructure of the noeplastic cells was characterized by more or less abundant plasmacytic-like endoplasmic reticulum with very frequent peculiar whorled configurations. Immunohistochemical methods revealed the abnormal protein production by neoplastic cells in different stages of differentiation. From this case and from the data of the literature it is concluded that the gamma HCD is due to a neoplastic proliferation of lymphoplasmacytic cells whereas the reticulum cells are never involved.

Aged↗
PubMed1980

Role of the T-cell system in glomerulonephritis induced in rats by human serum albumin (HSA). An immunological and morphological study.

The primary role of the T-cell system in immune-complex glomerulonephritis induced by intravenous weekly injections of human serum albumin (HSA) in rats has been demonstrated. The development of histological, ultrastructural and immunological glomerular alterations which are clearly recognizable in intact animals was prevented by neonatal thymectomy. In vitro tests of cellular immunity (LIF and PHA responsiveness) revealed a close relationship between the involvement of functioning T-cell subpopulations (at least T-helper) and the development of the classic glomerulonephritic pattern. In other words HSA antigen recognition by T lymphocytes, their cooperation with B lymphocytes, and the activation of the latter with related antibody response represent the immunological sequence which leads to the formation of the soluble circulating immune-complexes responsible for the glomerular injury. Our findings suggest that the same immunological sequence can represent the pathogenetic basis for many forms of glomerulonephritis in which T-dependent antigen stimulation is demonstrable. Our data are also discussed in the light of results obtained by others in immuneglomerulonephritis induced in nude athymic mice.

Animals↗
PubMed1979

Identification of rhinopharyngeal plasmacytic tumours by immunofluorescence on paraffin-embedded sections.

Solitary myeloma is an uncommon lesion which may occur in many organs, mainly in the rhinopharynx. This lesion usually appears as non-secretory, and it is constituted by either typical plasma cells or large and atypical cells resembling epithelial anaplastic cells. Therefore, and immunofluorescent method on paraffin-embedded sections may be applied to reveal the presence and the monoclonality of the intracytoplasmic Ig in the pathological cells. In this paper we report a study on 22 patients suspected to bear a rhinopharyngeal tumor investigated by immunofluorescence. Moreover, in 4 cases peripheral blood lymphocytes were also studied. The results are discussed on the basis of the modern immunological trends.

Adult↗
PubMed1978

Intracellular Ig identification on paraffin-embedded solitary plasmacytoid proliferation: a routine method to define the biological nature of plasma cells.

The difficulty to recognize morphologically the biological nature of the exuberant plasma cell proliferations is known by every pathologist. Therefore, an immunohistochemical method has been applied to routine paraffin-embedded sections of biopsies from 26 patients suspected to bear a solitary plasmacytoma. The method has allowed to distinguish between monoclonal (likely to be neoplastic) and polyclonal cases (likely to be reactive).

Clone Cells↗
PubMed1977

Waldenström-like immunocytic lymphoma with IgG serum M component.

A case of immunoproliferative disorder with clinical features of Waldenström's disease but with an IgG-k instead of IgM serum M component is described. The lymphocyte population in the bone marrow, blood and lymph nodes was studied by rosette test, culture with PHA and immunofluorescence staining. Most of the bone marrow lymphocytes had membrane IgG. This case represents an intermediate form between myeloma and Waldenström's disease, thus supporting the unitarian concept of all the immunoproliferative disorders.

Fluorescent Antibody Technique↗
PubMed1977

Primary gastric lymphoid proliferations: immunological criteria to distinguish gastric lymphoma from reactive hyperplasia.

A group of six patients with a marked gastric lymphoid "hyperplasia", two of these with generalised lymphoma and the others suspected for primary gastric lymphoma, were investigated to discriminate between simple reactive hyperplasias and lymphomatous proliferations. Microscopical and ultrastructural examinations were not useful for this purpose but immunological data can give evidence of a two-fold nature of gastric lymphoid hyperplasia. Some cases are without immunological disorders, others are met with peripheral blood signs of immunological alterations similar to those observed in malignant lymphomas and in chronic lymphocytic leukemia. Only these latter cases should, in our opinion, be regarded as primitive gastric lymphomas.

Adult↗
PubMedDate not supplied

Detection of multidrug resistance associated P-170 glycoprotein in previously untreated non small cell lung cancer.

Samples of Non Small Cell Lung Cancer (NSCLC) and normal bronchial tissue obtained in patients submitted to radical surgery and without previous exposure to cytotoxic drugs were investigated for the expression of P-170 glycoprotein using C-219 monoclonal antibody and an immunohistochemistry technique. In normal bronchial tissue the immunostaining was confined to the lumenal surface of the epithelium. Fifteen out of 86 NSCLC had more than 1/4 of examined cells positive for P-170 glycoprotein, but the heterogeneity of the expression ranged from rare scattered cells to a positive pattern for nearly all cells considered, without any relationship with pathologic and clinical prognostic variables.

ATP Binding Cassette Transporter, Subfamily B, Mem↗