Biomedical subjects

E Lenhartová

Publications and source records attributed to E Lenhartová.

11 recordsLinked to original sources

PubMed1992

Quantitative indices in paternity cases.

The study discusses the basic quantitative indices used as a standard method in foreign professional literature dealing with paternity cases. They are as follows: 1. mean probability of exclusion (PE) which characterizes the informative value of the experts opinions and is the same in all the disputes evaluated by this expert. 2. relative frequency of men chosen at random from the population and excluded at given phenotype of mother and child (RME). 3. probability of paternity (PP) for particular trio: mother-child-the accused man. Hereinafter the results of our studies in the HLA laboratory in Olomouc from 1976-1991 are introduced.

Blood Group Antigens↗

PubMed1992

HLA antibodies in renal transplant candidates.

The level of anti-HLA antibodies was followed in 155 patients of the chronic dialyzed program: against antigens HLA Class I and antigens HLA-DR representing HLA Class II. Correlation between the titre of these antibodies and additional factors were analyzed. The effect of pregnancy and consequently of sex was proved. Of significance were the number of dialyses undergone and the time of inclusion into the dialyses program. Positive correlation between the titre of both types of anti-HLA antibodies is so significant that it makes possible to concentrate on the more simple anti HLA I antibodies determination. The result of crossmatch test is of crucial importance when making decisions on suitable recipient of the kidney from the set of patients of dialyzed program (DP). The presence of anti-HLA alloantibodies can cause hyperacute rejection. The anti-HLA alloantibodies titre is regularly followed in DP patients. Several significant hypotheses can be verified in a large enough set of patients (hypotheses marked with ordinal numbers 1-5): 1. Only the titre of anti-HLA Class I antibodies is usually followed in DP patients. Some workplaces abroad consider it useful to follow even the anti-HLA Class II antibodies. Is anti-HLA I and HLA II antibodies titre in such a significant correlation that it is sufficient to follow only anti-HLA Class I? 2. Mechanisms of HLA antigen sensitization in DP patients are well known. We can verify the influence of transfusion number, allograft rejections and number of previous pregnancies on the anti-HLA antibodies titre.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1992

HLA-A and HLA-B typing: classification of errors.

The authors followed the frequency of errors in serologic identification of HLA-A and HLA-B antigens. They analyzed 205 paternity cases evaluated independently by two judicial experts (a revision of expert opinions). The specification of revealed discrepancies is demonstrated in the study and possible causes of their origin are analyzed. In the conclusions the authors recommend useful steps leading to the decrease of error frequency in HLA antigen typing. The HLA system--the major histocompatibility system of a man, has a broad utilization in the field of practical medicine. HLA compatibility should be respected in the choice of the couple donor-recipient for transplant therapy. HLA, as a highly polymorphic system, is successfully applied in forensic medicine. A strong association with some diseases makes it possible to utilize HLA as a supporting diagnostic sign. The commission for nomenclature of WHO at XI. International Histocompatibility Workshop (1991) recognized the following numbers of HLA specificities: 25 A, 55 B, 10 C, 21 DR, 9 PQ, 6 DP. At present HLA laboratories used mostly serological methods. The need to mutually differentiate HLA antigens which are similar due to their molecular structure puts an extraordinary demand on HLA laboratories. On one hand, a great number of HLA phenotypes exist in the population, which ensures the identification of every individual and the distinguishing of self-nonself components. On the other hand there appear the limited possibilities of HLA laboratories with relatively low numbers of diagnostic sera. We inquired how often errors occurred in HLA antigens determination. We chose a set of 205 auditing experts opinions of paternity cases for analyzing the problem.(ABSTRACT TRUNCATED AT 250 WORDS)

Child↗

PubMed1988