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Biomedical subjects

E Lederer

Publications and source records attributed to E Lederer.

175 records · Page 10Linked to original sources
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[Isolation and characterization of mitogens from Nocardia].

Two methods have been devised to isolate mitogens from Nocardia cells. (1) Lysozyme treatment of delipidated cells followed by fractionation of the soluble extract leads to a mitogen devoid of cell wall constituents. (2) Cell disruption followed by differential centrifugations shows that two mitogenic structures are present: the cell wall and fragments of the cytoplasmic membrane. The active component of the cell wall is the peptidoglycan wherefrom soluble mitogenic fractions have been obtained using bacteriolytic enzymes other than lysozyme. An intact glycan moiety seems necessary for mitogenic activity.

Centrifugation↗
PubMedDate not supplied

Relationship between chemical structure and adjuvant activity of some synthetic analogues of N-acetyl-muramyl-L-alanyl-D-isoglutamine (MDP).

Synthetic N-acetyl-muramyl-L-alanyl-D-isoglutamine, hereafter referred to as muramyl dipeptide or MDP, represents the minimal structure which can replace mycobacterial cells in Freund's complete adjuvant. This synthetic adjuvant can also enhance the humoral antibody response when administered to mice with an antigen in water by various routes including the oral route. The present paper reports some new results concerning the relationship between the structure and the adjuvant activity of MDP. Synthetic analogues of MDP were tested either in a water-in-oil emulsion in guinea-pigs or in saline in mice. The chemical modifications concerned essentially the D-glutamyl residue. The results obtained confirm the importance of maintaining the intact structure of the D-Glu residue and show that adjuvant activity can be abolished by certain modifications at the level of both carboxyl functions.

Adjuvants, Immunologic↗