Rapid immunodiagnosis of kidney rejection by using activation markers on kidney tubular cells and infiltrating T subset cells.
Explore the source record for details and available documents.
Antibodies, Monoclonal↗
Biomedical subjects
E Larsson
Publications and source records attributed to E Larsson.
Small bowel transplantation in the rat: a new technique.
Explore the source record for details and available documents.
Animals↗
[Remaining sucking needs in the children in developed countries leads to dummy and finger sucking].
Explore the source record for details and available documents.
Child, Preschool↗
Homologous type II collagen induces chronic and progressive arthritis in mice.
Native mouse type II collagen was used for immunization of DBA/1 mice. Arthritis developed exclusively in male animals and was characterized by a variable and delayed onset, a slow and progressive development, and frequent exacerbations of disease in several joints including those that were previously affected. Titers of anti-type II collagen autoantibodies were found not to correlate well with arthritis development. It appears that experimental arthritis induced with homologous type II collagen resembles rheumatoid arthritis in humans, both in certain clinical features and in the lack of correspondence between anti-type II collagen autoantibody titers and disease symptoms.
Animals↗
Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies.
Twenty monoclonal antibodies reactive with type II collagen were characterized as to their determinant specificity and their reactivity with cartilage-derived components. The monoclonal antibodies reacted with 7 different epitopes on the native type II collagen triple helical structure. Antibodies defining 3 of these epitopes occurred more frequently in sera from arthritic mice than in sera from nonarthritic mice. In vivo injection of some selected autoreactive antibodies caused synovitis, but in no case did it give rise to full-blown arthritis.
Animals↗
Virus p30-related protein in follicular fluids and C virus-like particles on the cell membrane of the human oocyte.
Fluids from 208 follicles were analyzed with respect to the concentration of estradiol and progesterone and to the presence of RD 114 retrovirus p30-related protein (rp), assuming that such a protein indicates the presence of C-virus particles in the follicular fluids. The results obtained showed that 60-80% of the follicular fluids within the same range of steroid hormone levels contained virus p30 rp. Electron microscopy showed 80-nm virus-like particles, more or less well preserved, in about half of the number of oocytes examined. Negative staining of purified follicular fluid particles showed ring-like structures with a size of 100-200 mm. It is concluded that the presence of virus p30 rp in follicular fluids is correlated neither to a certain steroid hormone concentration nor to the oocyte capability to become fertilized and cleave. The observed expression of an endogenous virus genome during oocyte maturation strengthens the hypothesis that this type of genome is active at early developmental stages.
Cell Membrane↗
Immunocytochemical demonstration of neuron-specific enolase for differential diagnosis of retroperitoneal tumours in children.
Six patients with malignant retroperitoneal tumours causing considerable diagnostic difficulties are reported. In a retrospective investigation the presence of neuron-specific enolase (NSE) was analysed immunocytochemically in specimens of these tumours. In all the patients who had finally got the definite diagnosis neuroblastoma, tumour specimens had a strong reaction for NSE whereas all other tumours did not react. An analysis of NSE already at the primary investigation could have led to an earlier definite diagnosis.
Child↗
The effect of dummy-sucking on the occlusion: a review.
Explore the source record for details and available documents.
Adolescent↗
Deoxythymidine kinase, a possible marker for monitoring activity of cytomegalovirus infection after renal transplantation.
Measurement of deoxythymidine kinase activity (S-TK) in serum is used as a marker for cytomegalovirus (CMV) infection following transplant surgery. A case is presented where a kidney transplant patient suffered a fatal CMV infection. The new antiviral drug Foscarnet was used to treat the infection. Retrospective analysis of S-TK showed a good correlation with the course of the disease, its treatment, recurrence and final outcome.
Antiviral Agents↗
Effect of dummy-sucking on the prevalence of posterior cross-bite in the permanent dentition.
In 1971 a comprehensive health inspection of all 4-year-olds in the County of Skaraborg was carried out. This also included a dental examination, in which the prevalence of cross-bite was related to the child's sucking habit. The aim of the present study was to investigate the development of the transverse relation between the arches in these 4-year-olds up to the age of 16. Among the 4-year-old dummy-suckers, the prevalence of cross-bite was five times as high than among children with no previous sucking-habit. In spite of this fact, it has not been possible in this study to establish a significant correlation between previous dummy-sucking and the development of cross-bite in the permanent dentition.
Adolescent↗
Ultrastructural characteristics of human oocytes fixed at follicular puncture or after culture.
The material consisted of 73 oocytes obtained at follicle aspiration in ovarian-stimulated women. Oocytes in various stages of maturation were either immediately fixed or cultured before fixation. Observations by transmission electron microscopy disclosed that 20% of the immature oocytes, which appeared normal in the light microscope, had commenced atresia, and one immediately fixed oocyte surrounded by a normal-appearing preovulatory cumulus mass was found to be degenerated. Further, a cumulus mass judged as preovulatory contained an oocyte possessing a germinal vesicle. Light microscopy is thus not always adequate for judging the condition of ova surrounded by cumulus cells. Cytoplasmic changes which were regarded as being related to oocyte maturation were a decrease in the number of vacuoles and multivesicular bodies, an increase in the volume of the endoplasmic reticulum, a clustering of mitochondria, and the appearance of aggregates of tubuli of the smooth endoplasmic reticulum surrounded by mitochondria and mitochondria-vesicle complexes. Certain features were found to cause misinterpretations at examination of oocytes in a light microscope. For instance, polar bodies can be mimicked by corona cells in the perivitelline space and pronuclei in oocytes by large mitochondria-vesicle complexes in the ooplasm. Consequently, not all oocytes in which polar body-like or pronuclei-like structures are observed are necessarily fertilized oocytes.
Cell Differentiation↗
Cell-type-specific pattern of myc protooncogene expression in developing human embryos.
The expression of viral oncogenes in cells transformed by acutely transforming retroviruses profoundly alters proliferation and differentiation in the target cell, suggesting that the cellular homologues of the viral oncogenes, the protooncogenes, have a role in normal cell proliferation and differentiation. To investigate the possible developmental role of protooncogenes in human embryogenesis, we have determined the spatial distribution of myc gene transcripts in early human embryos by using in situ hybridization of a labeled myc exon to thin sections. The results indicate a stage- and cell-type-specific regulation of c-myc gene expression in primarily epithelial cells of late first trimester embryos. Furthermore, the data suggest that the linkage between c-myc gene expression and cellular proliferation holds for only a restricted set of embryonic cells.
Cell Division↗
Emission of ink aerosol from ink-jet recorders.
As a follow-up of two patients found to be allergic to EKG*ink, a study was made, using spectrophotometric and light microscopic methods, of the factors influencing the emission of ink aerosol from ink-jet recorders used in electrocardiographs. Ink aerosol concentrations were only detectable by spectrophotometry under extreme conditions. However, the light microscopic method was sufficiently sensitive. At the slow paper speeds normally used (10-25 mm/sec) and under normal working conditions, the concentration of ink aerosol near the recorder was less than 0.1 microgram/m3; but aerosol emission increased exponentially with increasing ink pressure, i.e. increasing paper speed. At the highest paper speed studied (200 mm/sec), the aerosol concentration 36 cm from the ink nozzles was about 65 micrograms/m3. An increase in the distance from the ink nozzles to the chart paper resulted in a decrease in ink aerosol emissions. Aerosol dissipation was decreased by local exhaust ventilation of the ink-jet recorder. However, a protective shield designed to catch emitted ink aerosol only reduced the aerosol concentration at the measuring point at slow paper speeds. At higher paper speeds, the concentration increased. The average ink aerosol concentration near modern electrocardiographs is low, but some EKG operations may result in high peak concentrations. The formation of ink aerosol could be considerably reduced by changing the rheologic properties of the ink.
Aerosols↗
The prevalence and aetiology of prolonged dummy and finger-sucking habits.
Explore the source record for details and available documents.
Age Factors↗
Appearance of different lymphoid cells in synovial tissue and in peripheral blood during the course of collagen II-induced arthritis in rats.
The involvement of different sets of lymphoid cells in the development of collagen II-induced arthritis in rats was studied by means of immunohistochemical analyses on frozen sections of tissue from joint biopsy specimens taken at different phases of arthritis development. Particular attention was paid to cells involved in early pannus formation. Accumulation of anti-Ia-reactive cells close to the cartilage surface was seen early in the development of pannus, and the anti-Ia reactive cells could in later phases be seen infiltrating cartilage and crowding bone surfaces at sites of marginal erosion. With the help of monoclonal anti-T-cell subset antibodies and rabbit anti-rat immunoglobulin antiserum, it was demonstrated that synovial infiltration of T lymphocytes, particularly W3/25-reactive T "helper" cells, occurs very early in the development of arthritis, whereas a moderate increase of Ox 8-positive 'suppressor/cytotoxic' T cells and a small number of B cells and plasma cells are seen later in the course of the disease. Levels of Ia-expressing cells and of T cells belonging to different subsets were recorded in peripheral blood by means of immunocytochemical analyses on cell smears; no significant deviations from normal levels were seen during the development of arthritis.
Animals↗
Tissue distribution of T-lymphocytes and Ia-expressing cells in rat kidney grafts.
In a rat kidney transplantation model, DA kidneys were transplanted into Lewis rats. Syngeneic Lewis transplantations were studied as controls. Histologic evaluation was made, and immunohistochemical staining with a single-staining peroxidase-antiperoxidase technique on frozen sections was performed after 2, 4, 6, and 8 days. Antibodies for Ia antigen (Ox 6), 'suppressor/cytotoxic' cells (Ox 8), pan-T cells (W 3/13), and 'helper/inducer' cells (W 3/25) were used. Allogeneic grafts were almost completely rejected in 8 days. Syngeneic grafts also showed lymphocyte infiltrates, somewhat later than allogeneic ones, but were not rejected. In these infiltrates W 3/25-positive cells dominated, being even more numerous than W 3/13-positive cells. Relatively fewer Ox 8-positive cells were seen in the infiltrates of syngeneic than in allogeneic transplants. Infiltrates occurred later in the renal medulla than in the cortex. Perivascular infiltrates with cells of all investigated phenotypes were seen earlier in allogeneic grafts than in syngeneic ones. All tubular cells within one renal tubule appeared either Ox 6-positive or -negative. With time, all tubules in the allogeneic transplants became Ox 6-positive. Some increase of Ox 6-positive tubules was also seen in syngeneic transplants.
Animals↗
T lymphocytes in collagen II-induced arthritis in mice. Characterization of arthritogenic collagen II-specific T-cell lines and clones.
Collagen type II-specific long-term cultured T helper cells, derived from the DBA/1 mouse, have been established and characterized. Clones from these T-cell lines could be shown to recognize either species-specific or species-nonspecific determinants on the collagen type II molecule, including determinants on autologous mouse collagen. Induction of arthritis via transfer to both irradiated and normal syngeneic recipient mice was obtained with both collagen type II-specific T-cell lines and an autoreactive and collagen type II-specific T-cell clone. Fewer cells were needed to evoke arthritis in normal than in irradiated recipients. Cells from lines and the clone used for transfer were by immunocytochemistry shown to have T helper phenotype.
Animals↗