From central dressing room to central service department.
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Equipment and Supplies, Hospital↗
Biomedical subjects
E L Davis
Publications and source records attributed to E L Davis.
How Brooklyn centers aid community's emotionally ill.
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Humans↗
Central service: a department in transition.
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Equipment and Supplies, Hospital↗
Influence of finishing technique on microleakage.
Four different finishing techniques were used in a dentin bonding agent/composite resin restoration to evaluate the possible influence of finishing technique on microleakage. Results indicated that finishing technique affects the ability of the restorative system to resist microleakage. Under the conditions of this study, best results were achieved with a 30-fluted bur followed by a short wet polish. Samples finished dry with polishing disks demonstrated considerable microleakage. This study also suggests that homogeneous nonagglomerated microfilled composite resin does not require disk finishing.
Chi-Square Distribution↗
Evaluation of microleakage in tenure-based restorations.
This study evaluates the effects of six composite resins, which use three different curing systems, on the ability of Tenure to resist microleakage. The curing systems were a photoinitiated dual-cure system, a dual-cure system, and light-cure systems. A total of 39 human molars were used. Following 2,000 cycles on a thermocycler, specimens were exposed to 0.2 percent basic fuchsin dye for 24 hours and then examined for microleakage. Results indicated that the composite resin plays an important role in the ability of a restorative system to resist microleakage. The photoinitiated dual-cure system produced significantly less microleakage than light-activated systems. No difference in microleakage was found between light-activated systems and the dual-cure system.
Composite Resins↗
Microleakage evaluation in amalgam restorations used with bases.
Marginal leakage in amalgam restorations often precedes the development of secondary caries. One potential way to improve the marginal seal of such restorations, and thus minimize the risk of caries development, is to apply a glass-ionomer base prior to amalgam placement. This study compared microleakage resistance among amalgam restorations placed with and without light-cured glass-ionomer base materials. Preparations were made in extracted human molar teeth. Four groups were studied, including copal varnish, which was used as a control. All specimens were restored with amalgam. Results indicated significantly greater leakage at the cavity wall/base interface for restorations with a copal varnish cavity liner than for those with a glass-ionomer base. Leakage differences among bases were also found at the amalgam/base interface. Best results were obtained with a dual-cure resin-based system. These samples showed minimal leakage at both the cavity wall/base and the base/amalgam interfaces. These findings suggest that light-cured glass-ionomer bases can be effective in the prevention of microleakage in amalgam restorations.
Analysis of Variance↗
Profile and expectations of students entering general practice residency programs.
Although various studies have attempted to evaluate the success of general practice residency (GPR) programs in providing postdoctoral training, little work has investigated the motivation for senior dental students opting for such training compared with entering directly into private practice. Questionnaires were administered to 58 seniors at the State University of New York at Buffalo School of Dental Medicine 1 month before graduation to determine possible differences between those entering GPR programs and those planning to enter private practice. The survey also examined the goals of dentists about to enter GPR programs and compared them with the training goals determined by the Commission on Dental Accreditation. Although 75% of those entering GPR programs cited additional clinical experience as the reason for choosing this step in their careers, the most frequent response from those entering directly into private practice concerned the financial benefits. Also, of the respondents about to enter GPR programs, only one student indicated a desire for training in hospital dentistry. Although this survey was relatively limited in size, it appears that advanced instruction in the management of patients with complex health care problems is not one of the primary motivating factors for students considering residencies. This may indicate a need at the predoctoral level for greater instruction in changing health care demographics and in the training goals of general practice residencies, to continue to attract qualified dental graduates with goals compatible with those of GPR programs.
Attitude↗
Leakage patterns associated with glass-ionomer-based resin restorations.
This study compared microleakage patterns for glass-ionomer-cement-based resin systems with and without a separating agent placed between the glass ionomer and resin. Results indicated significant leakage (100%) at the dentin glass-ionomer interface for specimens without a separating agent. Those with a separating agent showed almost no leakage between the dentin and glass ionomer (10%) and some leakage at the resin/glass ionomer interface (40%). These results suggest that the forces of polymerization shrinkage are stronger than the chemical bond between glass-ionomer cement and dentin. This bond fails during resin polymerization, eliminating any supplementary retention gained through chemical adhesion to dentin and opening a pathway for microleakage.
Chi-Square Distribution↗
Adhesion of direct restorative materials.
The development of adhesive restorative systems has been a major focus in dentistry. Materials that can attach to tooth structure make it possible to take a far more conservative approach to restorative dentistry.
Adhesives↗
Dentin bonding agents and the smear layer.
The morphology and treatment of the smear layer are discussed. Also included are a review of dentin bonding agent classification and chemistry, and a summary of recent bond strength research results. Finally, the current status of dentin bonding agents is discussed.
Dental Bonding↗
Prevalence of oral defects among neonatally intubated 3- to 5- and 7- to 10-year old children.
Greater prevalence of oral abnormalities was found in two groups of children (ages 3 to 5 and 7 to 10 years) who had been intubated neonatally and were of low birth-weight than in same-aged children of average birth-weight who had not been intubated at birth. These abnormalities included enamel defects, high vaulted and/or grooved palates, posterior crossbites, and palatal asymmetry. In addition, these children were judged to have poorer speech intelligibility and greater speech nasality than the comparison group children. Prematurity and intubation were confounded in this study, allowing the possibility that prematurity alone is the causal factor in these differential results. However, the finding of localized enamel defects among intubated 3- to 5-year-olds, and greater prevalence of high vaulted palates and palatal grooving among intubated subjects in both age groups, provide strong support for intubation as a cause of both the occurrence and duration of oral defects among neonatally intubated children.
Analysis of Variance↗
Effect of dentinal pretreatment on bond strength between glass-ionomer cement and dentin.
Removal of the dentinal smear layer prior to placement of glass-ionomer cement is thought to maximize the strength of glass-ionomer cement/dentin bonding. This study evaluated the effect of three polyacrylic acid pretreatments on bond shear strength between glass-ionomer cement and dentin. Extracted human molars were divided into four groups of 30 specimens each. One group (the control) received no pretreatment. Specimens in the remaining groups were pretreated with one of three commercially available polyacrylic acid conditioners, used according to the manufacturers' recommendations. The results indicated significant differences in shear strength among pretreatment conditions. Since the manufacturers' recommendations varied, it is not clear if these results were due to differences in polyacrylic acid concentration or to other factors, such as application time or placement procedure.
Dental Bonding↗
Detection of cystic fibrosis delta F508 mutation by anti-double-stranded DNA antibody.
This study evaluated an enzyme immunoassay (EIA) as a screening tool for detection of the most common mutation (delta F508) in cystic fibrosis (CF). Guthrie card bloodspots were extracted to remove whole blood polymerase chain reaction (PCR) inhibitors. The washed filter paper was directly amplified under standard (98 bp amplicons) or modified PCR conditions (491 bp amplicons) for the delta F508 mutation. Monoclonal anti-double stranded deoxyribonucleic acid antibody was used to detect competent hybrid formation between PCR products and normal (N) and mutant (M) cDNA (deoxyribonucleic acid) probes coated to microtiter plate wells. Under standard conditions, mean relative color production (N/M) via an enzyme-linked horseradish peroxidase secondary antibody was 8.8, 0.6 and 0.04 for individuals normal, heterozygous and homozygous for the CF delta F508 mutation, respectively (n = 27). Comparison of EIA results to those obtained by tris-borate-EDTA/8 percent polyacrylamide gel electrophoresis (TBE-PAGE) yielded excellent correlation (100 percent) for all three genotypes (n = 27). In comparison to TBE-PAGE, the EIA was 5 to 10 fold more sensitive when serially diluted PCR samples were evaluated. Larger PCR products (491 bp amplicons) for the CF delta F508 mutation obtained under modified conditions were not resolved by TBE-PAGE. The EIA, however, demonstrated equal sensitivity to the 98 bp and 491 bp amplicons. Performance time for TBE-PAGE analysis was substantially shorter (25 percent) than the EIA (3.5 to 4 h and 4.5 to 5 h, respectively) when small batches of samples (n = 5) were analyzed. The TBE-PAGE was not, however, convenient for screening large numbers of PCR-amplified samples (n > 15).
Antibodies, Antinuclear↗
The effect of storage on Guthrie cards: implications for deoxyribonucleic acid amplification.
The effect of storage on (1) amplifiability of nucleic acid (present at low level) and (2) properties of whole blood polymerase chain reaction (PCR) inhibitors (present at high levels) in Guthrie card bloodspots was evaluated. Natural PCR inhibitors (protein, hemoglobin, iron) were selectively eluted from Guthrie cards (1 to 30 mo storage) under nondenaturing conditions and quantitated. The PCR was performed by direct amplification. It was found that PCR inhibitors become increasingly resistant to elution ("fixed") over time. For example, 600 micrograms protein, 1.87 au hemoglobin, and 374 ng iron were solubilized from 1 mo bloodspots. In contrast, only 137 micrograms protein (22 percent), 0.34 au hemoglobin (18 percent), and 147 ng iron (39 percent) were solubilized from 30 mo bloodspots. Fixation does not result from excessive desiccation since bloodspot weight 2.20 mg +/- 0.21 (1 mo) and 1.92 mg +/- 0.31 (30 mo) was not significantly changed (p > 0.05). The majority of protein was characterized as albumin, and two rbc metal-containing proteins, carbonic anhydrase and hemoglobin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Despite the presence of "fixed" PCR inhibitors, it was found that bloodspots stored 1 to 30 mo could be amplified for two regions (98 bp and 491 bp amplicons) encoding the delta F508 cystic fibrosis mutation. It is suggested that nucleic acid also becomes "fixed" to the filter paper matrix and accounts, in part, for the ability to amplify Guthrie cards by direct PCR and low yield of deoxyribonucleic acid (DNA) reported for microextraction methods.
Blood↗
Polymerase chain reaction amplification of Guthrie card deoxyribonucleic acid: extraction of nucleic acid from filter matrices.
This study evaluated two deoxyribonucleic acid (DNA) extraction methods for Guthrie card bloodspots. Our water-based extraction technique was compared with a commercial kit (GFX extraction system). In contrast to our nondenaturing method, the GFX system utilizes chaotropes to extract DNA. Extracted DNA is subsequently purified by selective elution from a glass fiber matrix. To evaluate DNA extraction, polymerase chain reaction (PCR) for a 491 bp region encoding the cystic fibrosis delta F508 mutation was performed and PCR products electrophoresed on polyacrylamide gels and stained with ethidium bromide. Amplification of GFX-extracted DNA required low sample volume (1 to 5 microL) indicating the presence of residual PCR inhibitors. The GFX volumes (1/20 to 1/4 punch) were comparable to our standard conditions and represented 0.16-0.80 microL whole blood (20 to 40 ng DNA). The GFX-extracted DNA was found to be stable (6 mo, -15 degrees C). Performance time for the GFX method was less than our standard water-based extraction (about 30 min), but more costly. The GFX extraction kit was adaptable for limited sample (i.e., extraction of a 3 mm punch yields 20 amplifications). The GFX extraction kit provides a useful means to standardize Guthrie card DNA extraction.
Blood Chemical Analysis↗