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Biomedical subjects

E Kim

Publications and source records attributed to E Kim.

At least 307 records · Page 17Linked to original sources
PubMed1977

Contralateral displacement of abdominal viscera by a retroperitoneal liposarcoma: ultrasonic demonstration.

Occasional reports have described contralateral displacement of the kidney, gall bladder, or liver by masses, but no ultrasonographic description of this phenomenon has been published. To our knowledge, this is the first report of contralateral displacement of all three structures by an encapsulated, well-differentiated low-grade liposarcoma. The significance to the ultrasonographer lies in the fact that he may be the first physician, as we were in this case, to have the opportunity to recognize the true anatomical relationships of all three organs and to suggest that this tumor is more likely to be benign or slowly growing rather than a highly infiltrative process.

Adult↗
PubMed1977

Treatment of the intestinal tuberculosis.

Twenty-seven cases of patients with intestinal tuberculosis were reported. Seventeen cases showed good response to antituberculosis chemotherapy. The numbers and size of ulceration, pseudopolyp and deformity of intestinal tract were checked by endoscopic and X-ray studies on comparative evaluation between prechemotherapeutic and postchemotherapeutic stage. In these cases, subjective symptoms were disappeared parallel with the marked improvement on X-ray and endoscopic findings. These data suggest that with current well planned antituberculosis chemotherapy program, tuberculous colitis is controlled fairly while tuberculous enteritis in controlled inadequately. Therefore, surgical procedures are commly indicated to the treatment of tuberculous enteritis.

Adult↗
PubMed1976

Primary and secondary carcinomata with focal nodular hyperplasia in a multinodular thyroid: case report.

A patient is described whose multinodular thyroid gland was found to have a primary papillary adenocarcinoma, a metastatic renal-cell carcinoma, and focal nodular hyperplasia. To our knowledge, this is the first case report of such an unusual combination. In a patient with known malignancy elsewhere, the possibility that a recent thyroid mass may be a metastasis should be considered.

Adenocarcinoma↗
PubMedDate not supplied

Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA.

5S ribosomal RNA sequences were determined for thirteen actinomycetes mainly representatives with the rare group B type peptidoglycan. The primary and secondary structure of the resultant sequences were of the type characteristic of Gram-positive bacteria with DNA rich in guanine plus cytosine. The sequencing and associated chemotaxonomic data provide compelling grounds for classifying actinomycetes with a group B type peptidoglycan in a single family. The family Microbacteriaceae fam. nov. is proposed to accommodate actinomycetes classified in the genera Agromyces, Aureobacterium, Clavibacter, Curtobacterium and Microbacterium.

Actinomyces↗
PubMedDate not supplied

Multiple infarcted regenerative nodules in liver cirrhosis after systemic hypotension due to septic shock: radiologic findings.

We describe a case of multiple infarcted regenerative nodules in a patient with advanced liver cirrhosis who had experienced an episode of septic shock. Sonography showed multiple hypoechoic or isoechoic nodules; contrast-enhanced computed tomography showed multiple, low-attenuating nodules with rim enhancement; and magnetic resonance imaging showed multiple nodules of low, iso-, or high signal intensity. Explanted liver showed coagulation necrosis of multiple regenerative nodules. Peribiliary cysts in chronic liver diseases, liver abscesses, spontaneous necrosis of hepatocellular carcinomas, and metastasis should be differentiated.

Adult↗
PubMedDate not supplied

Accurate prediction of the amount of in situ tumor in palpable breast cancers by core needle biopsy: implications for neoadjuvant therapy.

BACKGROUND: Neoadjuvant chemotherapy facilitates breast conservation in stage II breast cancer patients, whose primary tumors are assumed to be invasive because they are palpable. However, chemotherapy may not be indicated in the minority of patients whose clinically T2 tumors are completely or predominantly in situ. Almost all previous studies of core needle biopsy in breast cancer have been concerned with nonpalpable, mammographically detected tumors, and none have evaluated its ability to quantitatively determine the amounts of in situ and invasive disease. METHODS: From September, 1992 to December, 1997, core needle biopsy was performed on all patients presenting to the Kings County Hospital Breast Clinic with palpable breast masses. Carcinoma was present in both core needle biopsy samples and surgical specimens subsequently obtained from 95 of 99 patients. Each specimen was evaluated for tumor type, histologic grade, and the amounts of in situ and invasive carcinoma it contained, and the results from surgical and core needle biopsy specimens from the same patients were then compared. RESULTS: The surgical specimens of 14 patients had completely or predominantly in situ disease. Completely or predominantly invasive disease was present in 67 specimens, and the remaining 14 had significant amounts of both. The high level of agreement between the amounts of in situ and invasive disease in core needle biopsy and surgical specimens is indicated by Pearson and intraclass correlation coefficients of 0.91 (P < .001 and < .00001, respectively). Tumor type was correctly predicted by core needle biopsy in each case. Variables among these patients, including primary tumor size, interval between biopsy and surgery, or administration of neoadjuvant systemic therapy, did not alter agreement between core needle biopsy and surgical specimens. CONCLUSIONS: Core needle biopsy can identify palpable breast tumors that are predominantly or completely in situ, and, thus, avoid unnecessary neoadjuvant chemotherapy. It also can demonstrate that a tumor is predominantly invasive, but cannot rule out small invasive foci. For that purpose, complete surgical excision of the tumor is required.

Biopsy, Needle↗
PubMedDate not supplied

Biochemical and immunocytochemical characterization of GRIP, a putative AMPA receptor anchoring protein, in rat brain.

The mechanisms by which glutamate receptors are concentrated in brain excitatory synapses are believed to involve interactions between receptor subunits and postsynaptic anchoring or scaffolding proteins. Recently GRIP, a protein containing seven PDZ domains, was identified as an AMPA receptor binding protein and implicated in the synaptic targeting of AMPA receptors. Here we show that GRIP mRNA is also expressed in some tissues outside of the brain, including testis and kidney. Specific antibodies were raised to study GRIP protein. On Western blots, GRIP protein appears as a heterogeneous band (approximately 130 kilodaltons) which is expressed in widespread brain regions and throughout postnatal development. Biochemical studies reveal that GRIP is largely membrane associated and enriched in the postsynaptic density (PSD), though not as highly concentrated in the PSD as is PSD-95. By immunohistochemistry, GRIP is distributed in a somatodendritic pattern in neurons of adult rat brain, with especially prominent expression in a subset of interneurons.

Animals↗
PubMedDate not supplied

Effects of glycerol of beta-lactamase production during high cell density cultivation of recombinant Escherichia coli.

Recombinant Escherichia coli was cultured up to OD600 90 with glycerol fed by the DO-STAT method to produce recombinant beta-lactamase. Optimum feeding and induction conditions were determined. When glycerol was maintained at a high concentration (40-50 g/L) after isopropyl thiogalactoside (IPTG) induction at OD600 75, a 2-fold increase in the amount of soluble beta-lactamase was obtained. Similarly, a 3-fold increase was obtained by the addition of NaCl (0.4 M) at low glycerol concentration (10 g/L).

Escherichia coli↗
PubMedDate not supplied

Hg2+ removal by genetically engineered Escherichia coli in a hollow fiber bioreactor.

Escherichia coli cells engineered to express an Hg2+ transport system and metallothionein accumulated Hg2+ effectively over a concentration range of 0.2-4 mg/L in batch systems. Bioaccumulation was selective against other metal ions and resistant to changes in ambient conditions such as pH, ionic strength, and the presence of common metal chelators or complexing agents (Chen, S.-L.; Wilson, D. B. Appl. Environ. Microbiol. 1997, 63, 2442-2445; Biodegradation 1997, 8, 97-103). Here we report the characterization of the bioaccumulation system based on its kinetics and an isotherm. Bioaccumulation was rapid and followed Michaelis-Menten kinetics. A hollow fiber bioreactor was constructed to retain the genetically engineered cells. The bioreactor was capable of removing and recovering Hg2+ effectively at low concentrations, reducing a 2 mg/L solution to about 5 microgram/L. A mathematical equation that quantitatively described Hg2+ removal by the bioreactor provides a basis for the optimization and extrapolation of the bioreactor. The genetically engineered E. colicells and the bioreactor system have excellent properties for bioremediation of Hg2+-contaminated environments.

Bioreactors↗
PubMedDate not supplied

Social and psychological resources in the oldest old.

The purpose of this study was to compare mental health resources, activities of daily living, and social and economic resources in three age groups: centenarians, octogenarians, and sexagenarians. Two hundred and seventy-two older adults were assessed with the Older Americans Resources and Services Survey (OARS). Frequency analyses revealed that centanarians rated lower in social and psychological resources than the other two age groups.

Activities of Daily Living↗
PubMedDate not supplied

Postmortem distribution of zipeprol.

The abuse of zipeprol, an antitussive agent, is prevalent among young people in Korea. For its hallucinogenic effects, abusers have taken overdoses of the drug; thus, fatalities from zipeprol overdose have risen since 1991. In order to determine the postmortem distribution of zipeprol, tissues and blood from 23 decedents who had histories of drug abuse were examined. Homogenized tissue (1 g) and 1 mL blood were extracted by ethyl acetate. Cinnarizine was used as an internal standard. A Varian GC 4600 equipped with a thermionic specific detector was used to quantitate the drug using a DB-5 megabore column, and a Finnigan GC-MS model 4021 was used to obtain mass spectral identification of the extracts. The blood zipeprol concentrations varied from 2.3 to 38.3 micrograms/mL. The highest concentration of zipeprol was found in stomach tissue. Zipeprol concentrations in tissues were higher than the corresponding blood concentrations.

Adolescent↗
PubMedDate not supplied

Fatal zipeprol and dextromethorphan poisonings in Korea.

Zipeprol and dextromethorphan are abused together by young people in Korea to obtain a stronger hallucinogenic effect. Because large amounts of these drugs are taken for this reason, nine fatal poisonings due to zipeprol and dextromethorphan have been reported since 1993. In this paper, the concentration of drugs in the postmortem blood and gastric contents of these victims is examined. The determination and identification of the drugs in biological fluids were conducted by gas chromatography (GC)-thermionic specific detection and GC-mass spectrometry. Linear calibration curves and high recoveries were obtained. The blood concentrations of zipeprol varied from 1.3 to 28.6 micrograms/mL, and the concentrations of dextromethorphan ranged from 1.1 to 18.3 micrograms/mL. The concentration of zipeprol in the gastric contents ranged from 26.8 to 1384.8 micrograms/g, and dextromethorphan concentrations varied from 2.1 to 243.7 micrograms/g.

Adult↗
PubMedDate not supplied

A fatality due to injection of tiletamine and zolazepam.

A 22-year-old male with more than 28 needle marks on his right arm was found dead. First, he was suspected as a drug abuser. Blood, urine, spleen, and injection-site tissue was collected during autopsy. The blood and urine specimens were screened for drugs. Immunoassay studies did not show any illegal drugs. However, two unidentified peaks were isolated in both of these biological fluids by routine gas chromatography-flame-ionization detection (GC-FID) and thermionic specific detection. Additional gas chromatography-mass spectrometry analysis determined these two peaks to be tiletamine and zolazepam. These two agents are used in combination as veterinary anesthesia. The concentrations of these drugs in blood were quantitated by GC-FID and found to be 0.85 mg/L of tiletamine and 3.3 mg/L of zolazepam. In urine, tiletamine and its metabolite, 2-(ethylamino)-2-(2-thionyl) cyclohexanol, were identified to be present along with zolazepam. The concentrations of tiletamine and zolazepam in spleen were revealed to be 0.92 and 3.5 mg/kg, respectively. Injection-site tissue concentrations were determined to be 25.1 mg/kg tiletamine and 23.3 mg/kg for zolazepam. The cause of death in this case was determined to be due to the multiple drug intoxication of tiletamine and zolazepam.

Adult↗
PubMedDate not supplied

Evaluation of a synthetic wound dressing capable of releasing silver sulfadiazine.

A silver sulfadiazine-impregnated poly-L-leucine wound dressing, AgSD-medicated wound dressing, was evaluated for antibacterial capacity against Pseudomonas aeruginosa and cytotoxicity to human fibroblasts and human epidermal keratinocytes. This wound dressing contained 0.4 mg AgSD/cm2. Antibacterial capacity was examined on experimentally infected wound surfaces (3.4 x 10(4) P. aeruginosa organisms/gm) on the dorsum of mice. The AgSD-medicated wound dressing showed effective bacterial control. Cytotoxicity was examined on a monolayer of cells formed in culture dishes. Cellular damage was reduced by the controlled release of AgSD from the hydrophobic poly-L-leucine sponge matrix of the AgSD-medicated wound dressing. Cytotoxicity of the AgSD-medicated wound dressing was much lower than that of 1% AgSD cream.

Animals↗