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Biomedical subjects

E Kay

Publications and source records attributed to E Kay.

At least 19 recordsLinked to original sources
PubMed1997

Determination of testicular function after torsion by DNA flow cytometry of serial fine-needle aspirates.

OBJECTIVE: To determine the efficacy of DNA flow cytometric analysis of testicular percutaneous fine-needle aspirates in the assessment and follow-up of testicular function after torsion, and to determine the relationship between the duration of torsion and testicular injury. MATERIALS AND METHODS: Three groups of 15 adult rats underwent a 720 degrees torsion, with fixation of the mesorchial ligament, for 1, 3 or 5 h. Bilateral aspirations, performed 7, 21 and 35 days after torsion were examined by flow cytometry. Testes were harvested and evaluated histologically using Johnsen's scoring. RESULTS: Irreversible testicular injury occurred in all three groups of rats, with loss of function after 1 h and loss of viability after 3 and 5 h. The results from flow cytometry suggested significant contralateral testicular injury (P < 0.025) but this was not supported by the histological evaluation. There was a strong correlation between the testicular function assessed by flow cytometry and by Johnsen's scoring of histological specimens (r2 = 0.95). CONCLUSION: The assessment of testicular aspirates by flow cytometry allows testicular function to be followed after torsion in rats, and potentially in humans. Using DNA flow cytometry, the temporal course of the twisted testis in the adult rat was determined; contralateral testicular injury following the reversal of torsion could not be excluded.

Animals↗
PubMed1996

Dental health services research: what is it and does it matter?

Dentists working in the National Health Service offer an efficient service. However, changes in medical and dental technologies require all of us to monitor the effectiveness of both established and new treatments. Our clinical practices should become 'research laboratories' and we must grasp this opportunity to keep dentistry at the forefront of investigations into clinical care.

Cost-Benefit Analysis↗
PubMed1996

Prevalence of cystic fibrosis mutations in pregnancies with fetal echogenic bowel.

OBJECTIVE: To determine the prevalence of the most common cystic fibrosis mutations in pregnancies complicated by fetal echogenic bowel by using DNA testing. METHODS: Forty-five pregnancies with fetal echogenic bowel were studied prospectively for cystic fibrosis mutations. Using polymerase chain reaction, DNA from fetal amniocytes (n = 21), fetal blood (n = 5), or parental blood (n = 19) was amplified and tested for delta F508, G551D, G542X, and 621 + 1G-->T cystic fibrosis mutations, which account for about 85% of the mutations in the British population. In selected cases, further mutations were tested according to the parental ethnic background. RESULTS: Only one of the 26 fetuses screened was heterozygous for cystic fibrosis mutations. Among 38 parental samples screened from the remaining 19 pregnancies, cystic fibrosis mutations were detected in two cases, only one of the parents being a carrier in each case. The prevalence of cystic fibrosis carrier status in fetal and parental samples (1:26 and 1:19, respectively) is within the expected prevalence in the British population (1:25). No fetuses were affected by cystic fibrosis in this series, but five were found to have growth restriction, two trisomy 21, two congenital infection, and two bowel obstruction. CONCLUSION: Our results suggest that ultrasonographic detection of fetal echogenic bowel is not associated with an increased prevalence of cystic fibrosis mutations in pregnancies at low risk for this disease.

Adolescent↗
PubMed1996

A method of estimating tooth life expectancy.

One of the most important outcome measures for dental services is the increase in tooth life expectancy which is brought about by the interventions provided. Thus, a convenient and accurate index of tooth survival is critical both to clinical decision making and to more general assessments of public dental health policies. This paper describes a simple method of approximating tooth life expectancy (TLE). The method is based on the assumption that tooth survival follows a simple declining exponential function. Assuming a constant mortality rate, TLE is the integral of the survival function, expressed as S(t) = e-mu t, where t is time and mu the constant mortality rate. Using 3-year tooth-specific mortality rates for 491 subjects aged 50 years or more, tooth-specific life expectancies for the population as a whole where found to range from 27 years for upper canines to 71 years for lower incisors. Individuals with a mean periodontal attachment loss greater than 4 mm and people on low incomes had significantly lower tooth life expectancies than their periodontally fit and more wealthy counterparts. This technique can be used to obtain disease-specific or intervention-specific tooth life expectancies. Thus, gains in TLE for individuals with different oral, social and behavioural characteristics can be calculated. The declining exponential approximation of tooth life expectancy has the potential to become a powerful tool in the evaluation of dental services and treatments.

Cuspid↗
PubMed1996

Perceptions of orthodontic treatment need: Receiver Operating Characteristic analysis.

This study aimed to investigate the variations in dentists' perception of need for orthodontic treatment. Sixteen dentists viewed forty clinical vignettes and recorded their decisions according to the certainty with which they would offer treatment for each case. Receiver Operating Characteristic (ROC) analysis was performed on this data, using the Index of Orthodontic Treatment Need (IOTN) as the "gold standard'. The data suggest that dentists treatment decisions do not concur with current guidelines in use in the UK.

Attitude of Health Personnel↗
PubMed1995

Clinical decision making--an art or a science? Part V: Patient preferences and their influence on decision making.

Part IV of this series introduced the idea that the value a patient places on an outcome has an important influence on the decision making process. This article considers this concept further. It argues that dentists should assess a patient's preferences and consider these before deciding on a treatment option. The article explains that by enumerating all the treatment options and possible outcomes, it is possible to assess the value placed on each result. Similarly an assessment is crucial if the dentist aims to achieve the optimum treatment result--one which is regarded as the most favourable outcome by the patient.

Attitude to Health↗
PubMed1995

Clinical decision making--an art or a science? Part IV: Assessing risks and probabilities.

Deciding whether a patient requires any dental treatment will depend on an assessment of the risks involved. How likely is it that the condition will get worse if it is left untreated? How likely is it that a particular treatment will be successful? This article looks at risk assessment and examines how the relative risks of a particular course of action might be communicated. It argues that dentists should be able to explain clearly and concisely what is likely to happen to the patient.

Decision Support Techniques↗
PubMed1995

Clinical decision making--an art or a science? Part III: To treat or not to treat?

Part II of this series examined the two principle sources of variation: variation stemming from perceptual differences such as diagnostic errors, and variation stemming from judgmental differences such as different treatment strategies. This paper looks at judgmental variation in more detail, considers the nature of practice variation, and asks whether such variations are a sign of good or bad clinical practice.

Decision Making↗
PubMed1995

Clinical decision making--an art or a science? Part II: Making sense of treatment decisions.

Clinical decisions made by dentists can vary considerably. Although there has been a tendency in the media to attribute these differences between practitioners to deliberate unethical practice, as Part I concluded, variations in decision making are a result of the complexity of assessing the risks of different treatment options and evaluating the outcomes of treatment. This article looks at why dentists can make different decisions when faced with identical cases (variation), why sometimes the 'correct' decision is not made (error), and also looks at what other issues affect treatment decision making once disease has been perceived.

Clinical Competence↗
PubMed1995

Clinical decision making--an art or a science? Part I: An introduction.

In order to make the most appropriate decision in a given set of circumstances, an understanding is needed of how people formulate decisions on the basis of their surroundings. Using a case history, this article introduces the factors involved in clinical decision making and highlights the relevance of those factors in the decision making process. These will be discussed in depth in later sections of this series.

Adult↗
PubMed1995

DNA quantification is technically feasible and of value in cervical smear samples: possible applications for determination of progression in low grade dyskaryosis.

This study evaluates the feasibility of DNA analysis of cervical intraepithelial neoplasia III (CIN III) lesions on cervical smear and formalin-fixed paraffin-embedded tissue (FFPET) blocks with a view to extending this type of analysis to milder grades of dyskaryosis. DNA ploidy was determined by image analysis using a CAS 200 Image Analyser. Seventeen patients with a diagnosis of CIN III were studied. Results show that all smear and tissue samples were non-diploid with nine aneuploid and eight tetraploid lesions. In 6/7 patients whose smears and corresponding biopsies were examined there was complete agreement as to the DNA profile. We conclude that DNA quantification is technically feasible in archival, routinely prepared cervical smears. This technique should now be applied to CINI and CINII cervical smears to determine if it is of value in identifying those lesions that will progress to CIN III. This study is particularly timely with the possibility in the near future of estimation of ploidy by image analysis using instruments such as the Highly Optimized Microscope Environment (HOME) system.

Aneuploidy↗
PubMed1995

The relevance of health state utilities to lower third molar surgery.

A study of the attitudes of 102 arts and science undergraduate students was carried out to establish relative utility values, that is the participants own assessments, for possible outcomes of surgical intervention and non-intervention in lower third molar management. Using a standardised visual analogue scale, respondents were asked to rate 17 outcomes of treatment presented to them by means of short descriptions (vignettes). Ranking of mean utility values showed that post operative complications were considered to be a greater handicap than the sequelae of leaving third molars in situ. The respondents considered that the effects of irreversible lingual and inferior dental nerve damage reduced health to a major degree, and that normally encountered post operative complications such as moderate pain, swelling and trismus were a greater handicap than recurrent mild/moderate pericoronitis.

Adult↗
PubMed1995

Public health aspects of third molar surgery. The effect of surgeons' treatment thresholds on efficiency and effectiveness.

Disease associated with third molars is being recognised increasingly as a public health problem in terms both of rising incidence and of appropriate use of resources. Since minor alterations in treatment philosophy can have a substantial impact on the efficiency and effectiveness of oral surgery services, this study examines the sensitivity and specificity of oral surgeons' decisions and uses Receiver Operator Characteristics (ROC) analysis to investigate their ability to detect and make appropriate judgements about teeth which need extraction according to established criteria. The study shows that the seniority of the surgeon making the treatment decisions had no significant effect on the decisions made and also demonstrated wide variations between the treatment plans made by individual clinicians. It is clear from this study that if an efficient and effective service for the treatment of third molar disease is to be commissioned, policy decisions about criteria for intervention must be made by health service purchasing authorities, adhered to by secondary care providers, and shared with the primary care practitioners who refer patients for treatment.

Decision Making↗
PubMed1994

Oxygen free radical activity in experimental colonic carcinoma.

Oxygen free radical activity has been implicated in carcinogenesis through DNA lysis. This study measured chemiluminescence and superoxide dismutase (SOD) inhibition as an index of oxygen free radical activity in azoxymethane-induced colonic carcinoma. After 3 weeks of acclimatization, ten Fisher 344 rats were injected with azoxymethane (7 mg kg-1 week-1) over 10 weeks. All animals were killed at 37 weeks and colonic mucosa (5-25 mg wet weight) was sampled from sites of histologically confirmed gross tumour and sites 3-5 cm distant from tumour as well as from ten paired normal controls. The samples were processed in buffered luminol and chemiluminescence was measured in a luminometer. Each sample was then placed in a solution of SOD for 3 min before a second chemiluminescence estimation. Mean(s.e.m.) chemiluminescence from control mucosal samples decreased from 8.1(1.0) to 3.9(1.1) mV per 100 mg tissue (P < 0.05) after incubation in SOD, suggesting that oxygen free radical activity was being measured. There was an increase to 20.2(0.9) mV per 100 mg in azoxymethane-treated colon remote from tumour and to 53.9(2.1) mV per 100 mg at tumour sites; both were reduced significantly after incubation with SOD to 6.7(0.7) and 11.0(0.8) mV per 100 mg respectively (P < 0.001). Chemiluminescence was significantly increased in azoxymethane-induced colonic carcinoma, both remote from and maximally at the tumour site, suggesting a field change in carcinogenesis associated with oxygen free radical activity.

Animals↗