Biomedical subjects
Publications and source records attributed to E Karasek.
Cyclic AMP concentrations in a non-toxic nodular goiter were measured by means of the radiocompetitive protein-binding assay. It has been shown that cold thyroid nodules contain a higher concentration of cAMP than the macroscopically normal, extra-nodular thyroid tissue.
Effects of dopamine and thyroliberin on the cyclic AMP concentration in the rat anterior pituitary gland were investigated in vitro. Dopamine by itself had no effect on the anterior pituitary cAMP concentration but inhibited the cAMP-accumulating effect of thyroliberin.
The concentration of adenosine 3',5'-monophosphate (cAMP) was investigated in the rat pineal organ after bilateral orchidectomy. Orchidectomy caused a decrease in pineal cAMP concentration.
Concentration of adenosine 3',5'-monophosphate (cAMP) was investigated in the male rat pineal organ after a treatment with human chorionic gonadotropin (HCG). HCG significantly decreased cAMP concentration in the pineal organ.
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Crude tissue extracts prepared by homogenization and ammonium sulfate precipitation of mouse brains as described by Gospodarowicz et al. (1975) for brains and hypophyses of cattle have also been found to contain fibroblast growth factor-activity. In the presence as well as in the absence of calf serum in the medium the survival and proliferation of primary mouse embryo fibroblasts in culture were promoted as documented by dose-dependent increase of cell number and protein content during an incubation period of 48 hours. In the case of serum-free incubation also a significant rise of thymidine incorporation induced within 24 hours by the extract was noted. In contrast, the proliferation of primary chicken embryo fibroblasts or transformed mouse cells of the line "L" was not stimulated. Some possibilities for a practical use of such extracts are discussed.
The effect TRH and thyroxine (T4) in vitro on the content of cAMP in the anterior pituitary gland in rat have been investigated. Thyroxine did not significantly affect this concentration, but TRH produced approximately a two-fold increase in the pituitary cAMP content. Preincubation of the anterior pituitary together with T4--under the conditions when the latter inhibited both proliferative and secretory response of the gland to TRH--did not prevent the elevation of cAMP due to TRH. It is assumed that T4 must have exerted its inhibitory effect outside of the cyclic AMP system.
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The carbohydrate binding glycoprotein Anti-AHP binds to the plasma membrane of the (transformed) L-cell both in agglutination reactions and by the immunofluorescence technique. On the surface of (normal) mouse embryo fibroblast cells Anti-AHP receptors were not detectable. Anti-AHP is suitable as another marker for L cells. The random distribution of the binding sites at 4 degrees C changes with elevated temperatures (clustering, capping of sites). Anti-AHP inhibits the growth of L cells much more than that of fibroblasts. It does not act as a mitogen for the investigated cells.
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