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Biomedical subjects

E Kajii

Publications and source records attributed to E Kajii.

At least 91 records · Page 5Linked to original sources
PubMed1991

A new genetic polymorphism in human platelet polypeptides detected by two-dimensional electrophoresis.

We described a new genetic polymorphism of human platelet polypeptide, detected by two-dimensional polyacrylamide gel electrophoresis followed by silver-staining. The polymorphism was tentatively designated thrombocyte B (ThB) with a molecular weight of 34 kDa and isoelectric point of 4.7-4.8. In this polypeptide, three different electrophoretic types (1-1, 1-2, 2-2) were identified. Family and population studies indicate that the three phenotypes of the polypeptide are determined by two common alleles at a single autosomal locus. In a Japanese population, the gene frequency of ThB 1/ThB 2 was 0.74/0.26. The ThB polypeptide was not found in other blood cells and decreased or disappeared during the preparation or storage of platelets.

Alleles↗
PubMed1991

[Multiple myeloma with hemolytic anemia and thrombocytopenia].

We report a 59 year old female patient who was diagnosed as having IgG kappa myeloma with hemolytic anemia and thrombocytopenia simultaneously. Although M-protein was suspected to contribute to the hemolysis, the IgG purified from the patient's serum did not bind to red blood cells. Therefore, massive non-specific binding of M-protein to blood cells might contribute to high levels of red blood cell-associated IgG and platelet-associated IgG in the patient.

Anemia, Hemolytic↗
PubMed1991

The effect of the Nepenthes alata extract on the cold agglutinin-associated antigens.

Nepenthes alata is a tropical insectivorous plant with pitchers. After treating human red blood cells (RBCs) with the extract from the Nepenthes pitchers, the antigenicities of I, IF, Lud, and Om on the RBCs disappeared. As these antigens are associated with cold agglutinins, this extract appears to contain several decomposing enzymatic activities that work against glycolipids and glycoproteins. These activities of this extract, however, ceased on heating at 100 degrees C for 15 min.

Agglutinins↗
PubMed1990

Two-dimensional polyacrylamide gel electrophoretic pattern of duodenal tumour proteins.

We investigated the protein pattern of a surgically resected tumour in a case where it was difficult to distinguish between duodenal and pancreatic cancer. The investigation was performed using two-dimensional polyacrylamide gel electrophoresis with silver staining. Samples of the duodenal tumour, normal duodenal mucosa and normal pancreatic tissue from the same patient were compared. Each gel had ca. 250-300 protein spots, and the tumour sample pattern more closely resembled that of normal duodenal mucosa than that of pancreatic tissue, suggesting that the tumour had arisen from duodenal mucosa. There were three proteins identified only in the tumour sample gel, and these may have been tumour-specific proteins.

Aged↗
PubMed1990

Esophageal carcinoma-associated proteins detected by two-dimensional polyacrylamide gel electrophoresis.

Patterns of proteins of five surgically resected esophageal carcinomas were studied by two-dimensional polyacrylamide gel electrophoresis with silver staining. The samples of normal esophageal mucosa and esophageal carcinoma from the same patient were compared. Each gel had ca. 300 protein spots and had a similar pattern of proteins. Four spots were observed in all of the esophageal carcinomas that were not present in any of the normal mucosae. The molecular weights and isoelectric points were 46,000 and 5.3, 46,000 and 5.2, 36,000 and 4.7 and 33,000 and 5.1, respectively. One spot was observed in all of the normal mucosae but not in any of the esophageal carcinomas. Its molecular weight and isoelectric point were 27,000 and 5.3, respectively.

Aged↗
PubMed1990

Expression of major blood group antigens on human erythroid cells in a two phase liquid culture system.

In order to examine the sequential expression of major blood group antigens on human erythroblasts, a selective two phase liquid culture system for erythroid progenitors was established. After mononuclear cells obtained from peripheral blood were cultured in the presence of phytohemagglutinin stimulated-leukocyte conditioned medium (PHA-LCM) for 7 days (the first phase), nonphagocytic cells were recultured under hypoxic culture conditions containing 30% fetal calf serum, 1% bovine serum albumin, 300 micrograms/mL transferrin and 2 U/mL recombinant erythropoietin (the second phase). Mature (orthochromatic) erythroblasts were observed on day 4 of the second phase, and reached 57.1 +/- 3.1% of total cells on day 8, followed by the appearance of denucleated red cells, equivalent to mature red cells in peripheral blood. Hemoglobin contents reached the level of 16.8 +/- 0.7 micrograms/10(6) cells on day 8. Flow cytometric analyses revealed that, on day 3 of the second phase, cells became blood type M-positive, corresponding to the maturation of erythroid cells. Regarding the expression of ABH blood group antigens, a small number of blood type H-positive cells were initially detected on day 0 of the second phase, while blood type A-positive cells, which essentially were not observed on day 0, increased gradually corresponding to the extent of erythroid maturation. In the present system, Lewis and P1 blood group antigens were expressed at day 5 of the second phase, although autologous plasma was required to determine the expression of Lewis blood group antigens. This culture system is beneficial for studies on normal and abnormal human red cell membranes, because the erythroid progenitors in human peripheral blood were used, and a reasonable number of erythroid cells (0.5 to 1.5 x 10(7] was obtained with good maturation.

ABO Blood-Group System↗
PubMed1990

[A case of pulmonary thromboembolism due to circulating lupus anticoagulant].

A 22-year-old man was admitted because of hemosputum and progressive dyspnea with 3 attacks of chest pain and dyspnea over the previous 4 months. Chest roentgenography showed pulmonary infarction of the left lower lobe, and the diagnosis was confirmed by pulmonary perfusion and inhalation scintigraphy and pulmonary arteriography. Thrombolytic therapy was performed, but no significant effect could be obtained and anticoagulant therapy was performed continuously. No deep-vein thrombosis could be seen. He was considered to have autoimmune hemolytic anemia with lupus anticoagulant on the basis of auto-antibody data. Lupus anticoagulant is an antibody to phospholipid, and it is suggested that a decrease in the production of prostanoid in the endothelium causes thrombosis. In this case, as the patient showed a low level of 6-keto-PGF1 alpha in the blood, it is suggested that one of the etiological factors of pulmonary thromboembolism is a disorder of prostacyclin production in the endothelium, causing thrombosis by lupus anticoagulant.

Adult↗
PubMed1990

Blood group antigens transformed by proteinase K-treatment and the discovery of a natural autoantibody of these treated red blood cells.

Proteinase K-treatment of red blood cells either diminished or abolished the antigenic activities of glycophorin A and glycophorin B, and revealed the presence of a cryptic antigen that was recognized by antibody naturally existing in the autoplasma. About ninety five percent of all healthy persons have this autoantibody belonging to the IgM classification, whose titer ranges from 2 to 32. The activity of this autoantibody was absorbed by histidine and glutaminic acid. We were able to isolate this autoantibody from the plasma by means of an alkaline elution method and the autoantibody did not agglutinate chymotrypsin-treated red blood cells and red blood cells treated with chymotrypsin, following proteinase K-treatment. These results indicate that after proteinase K-treatment this autoantibody may not have an affinity for glycolipids, but for proteins digested by chymotrypsin.

Autoantibodies↗
PubMed1990

Characterization of a monoclonal crossreacting anti-A, B antibody.

Ten monoclonal antibodies (MAbs) have been produced from mouse-mouse hybridomas raised to dispase-treated human group AB red blood cells (RBCs). One of these antibodies, T-1435A, reacted with the RBCs of the human group A, B, and AB, but not to the RBCs of group O. The reactivity of T-1435A was specific to a single anti-A, B but not to a mixture of an anti-A and an anti-B. Also, T-1435A reacted strongly with protease- and neuraminidase-treated RBCs, and the susceptibility of its corresponding antigen was markedly reduced by alpha-galactosidase treatment. T-1435A agglutinated group A RBCs from chimpanzees, and its reactivity to RBCs was recognized in tamarins and in owl monkeys. However, this antibody demonstrated a stronger affinity for human RBCs. Further, using A- or B-transferase, human and chimpanzee RBCs that transferred from group O to A or to B were agglutinated by T-1435A. Finally, the corresponding antigen appears to have a structural determinant that fits the T-1435A combining site. Therefore, this monoclonal antibody is useful for determining the ABO blood-grouping when used as a conventional reagent that segregates group O RBCs from the RBCs of the other groups.

ABO Blood-Group System↗
PubMed1990

Behavior of genetic markers in recipients after bone marrow transplantation and problems in forensic medicine.

The authors report studies on four pairs of donors and recipients in bone marrow transplantation (BMT). A broad range of gene markers at 41 gene loci, including 11 red blood cell markers, 5 human lymphocyte antigen (HLA) types, 12 serum protein markers, 5 red cell enzyme markers, and 8 salivary markers were evaluated before and after BMT over 2 months. As a result, 9 out of 41 gene loci of genetic markers in recipients were transformed into the donor type. BMT between family members may lead to transformation of gene markers, but within a pattern compatible with family inheritance patterns, and no genetic paradox will be found in later surveys of familial genetic relationships. However, in a personal identification system in forensic medicine using genetic markers as an index, the appearance of a phenotype incompatible with a blood relationship is possible after BMT with a non-blood-relative donor. This result is similar to the inheritance pattern observed after artificial insemination by a donor's semen (AID), a more complete out-of-family cross.

Blood Group Antigens↗
PubMed1989

Application of analytical isotachophoresis in the study of platelet membrane proteins in patients with chronic myeloproliferative disorders.

The supernatants of dispase-treated platelets from normal persons and patients with myeloproliferative disorders were analysed by capillary isotachophoresis. The examined myeloproliferative disorders were three cases of chronic myelogeneous leukemia, one case of chronic neutrophilic leukemia, one case of essential thrombocythemia and one case of polycythemia vera. An additional peak was revealed in the samples from the patients with chronic myelogeneous leukemia. Platelets from the other three patients showed no additional bands. Isotachophoresis will be useful not only to analyse the superficial peptide on the cell membrane but also to define the peptide associated with malignant transformation.

Amino Acids↗
PubMed1989

A cold agglutinin: Om.

An IgM kappa cold agglutinin reacted with cord blood as well as with adult red blood cells (RBC). The agglutinin reacted strongly with papainized and neuraminidase (RDE)-treated RBC. It resembled anti-Me cold agglutinin in these serological findings. However, the activity of this agglutinin against RBC was markedly decreased in the presence of human milk. The data suggest that the agglutinin is directed against a new glycolipid antigen common to human RBC, termed Om antigen.

ABO Blood-Group System↗
PubMed1989

[Macrophages and erythrocytes].

The mechanism by which senescent red blood cells (SRBCs) are eliminated from circulation by the reticuloendothelial system was investigated using the newly developed phagocytosis assay. Phagocytosis of SRBCs and Vibrio cholera neuraminidase (VCN) treated RBCs by autologous macrophages were significantly higher than that of unfractionated RBCs. The phagocytosis of VCN-treated RBCs was enhanced by pre-treatment of RBCs with freshly prepared autologous serum, but not with heat inactivated serum. These results suggest that the erythrophagocytosis is mediated by lectin-like receptors and complement receptors which are present on the surface of macrophages. We also studied the erythrophagocytic capacity of macrophages in the presence of tumor necrosis factor (TNF) as a model of the anemia due to acute inflammation. The phagocytosis of VCN-treated RBCs was enhanced with the addition of a small amount of TNF (1 U/ml). This result suggests that TNF enhances the destruction of damaged RBCs by activating the reticuloendothelial system.

Adult↗