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E Jantzen

Publications and source records attributed to E Jantzen.

At least 55 records · Page 3Linked to original sources
PubMed1982

Fatty acid analysis for differentiation or Bordetella and Brucella species.

The fatty acid composition of Bordetella pertussis (13 strains), B. parapertussis (3 strains), B. bronchiseptica (6 strains), Brucella abortus (6 strains), B. melitensis (4 strains) and B. suis (5 strains) was determined. Both genera contained straight-chain saturated and mono-unsaturated acids as well as cyclopropane substituted isomers, but the overall differences between the two genera were distinct. The Brucella species contained exclusively C16 to C19 acids. Minor amounts of hydroxylated acids (tentatively identified as 3-hydroxy-hexadecanoate and 3-hydroxy-octadecanoate) could only be detected after thin-layer chromatography and substantial concentration. The Bordetella species, on the other hand, contained a number of both non-hydroxylated and hydroxylated fatty acids of chain-lengths varying from C10 to C19. Intra-generically, no clear distinction between the Brucella species could be detected. The three species of Bordetella, on the other hand, were clearly distinguished by their fatty acid patterns. B. pertussis did not contain cyclopropane fatty acids encountered in significant amounts in the two other species, and B. bronchiseptica was characterized by containing 2-hydroxy-dodecanoate in significant amounts.

Bordetella↗
PubMed1981

Fatty acids of Fusobacterium species: taxonomic implications.

Fatty acids of Fusobacterium species were examined by gas-liquid chromatography. Fusobacterium nucleatum, F. necrophorum, F. mortiferum, F. gonidiaformans and F. varium showed similar patterns, characterized by the presence of 3-hydroxytetradecanoate, n-tetradecanoate, hexadecenoate, n-hexadeconoate, ocadecenoate, n-octadecanoate and a component having the properties of octadecadienoate. Fusobacterium nucleatum contained 3-hydroxyhexadecanoate as a distinctive character. Simpler fatty acid patterns characterized by the absence of 3-hydroxytetradecanoate and other hydroxy fatty acids were observed in F. plauti, the single strain of F. prausnitzii and in the majority of strains classified as F. russii and F. naviforme. Neither methyl-branched nor cyclopropane fatty acids could be detected in any of the strains examined. In addition to fatty acid methyl esters, the chromatographic profiles of all species except F. mortiferum, F. gonidiaformans and F. naviforme contained substantial amounts of fatty aldehyde dimethyl acetals of chain lengths C14 to C18.

Chromatography, Gas↗
PubMed1980

Cellular fatty acid composition of Haemophilus species, Pasteurella multocida, Actinobacillus Actinomycetemcomitans and Haemophilus vaginalis (Corynebacterium vaginale).

The fatty acid composition of 35 Haemophilus influenzae strains was found to be grossly similar and characterized by relatively large amounts of 14:0, 3-OH-14:0, 16:1 and 16:0. The three C18 fatty acids 18:2, 18:1 and 18:0 were also present, but in much lower concentrations. This general pattern was also found for most of the other species of Haemophilus examined (H. aegyptius, H. aphrophilus, H. canis, H. gallinarum, H. haemolyticus, and H. parainfluenzae). Small but distinct quantitative discrepancies were detected for H. ducreyi and the haemin-independent species H. paraphrohaemolyticus, H. paraphrophilus and H. suis. Actinobacillus actinomycetemcomitans was found to be indistinguishable from H. influenzae. Pasteurella multocida also exhibited a fatty acid pattern closely related to that of Haemophilus, but could be distinguished by its higher concentration levels of the C18 fatty acids. The fatty acid pattern of H. vaginalis was considerably different from those of the other species examined. This species lacked 3-OH-14:0 and 18:2 and contained small amounts of 14:0 and 16:0, whereas 18:1 and 18:0 were the major constituents.

Actinobacillus↗
PubMed1979

Cellular fatty acid composition of Francisella tularensis.

Several unusual fatty acids characterized strains of Francisella tularensis. Long-chain (C20-C26) acids and the hydroxy acids 2-hydroxy-decanoate, 3-hydroxy-hexadecanoate, and 3-hydroxy-octadecanoate appeared to be of special diagnostic value.

Decanoates↗
PubMed1977

Occurrence and patterns of waxes in Neisseriaceae.

Forty-five strains classified in the family Neisseriaceae were analysed for wax esters by gas-liquid chromatography. The amounts and types of waxes varied between the taxa. Waxes were not detected in 16 strains of 'true neisseriae' (genus Neisseria) or in two strains of Kingella, but they were found in all 'false neisseriae', in all species of Moraxella except Moraxella phenylpyrouvica, in five out of 10 strains of Acintobacter, and in all strains of a group of psychrophilic, oxidase-positive organisms. The chain lengths of the wax esters ranged from C24 to C42, with C36 predominating. In all taxa, esters with even numbers of carbon atoms constituted 70 to 100% of the total. Saturated, mono-unsaturated and diunsaturated waxes were found. Acinetobacter strains were characterized by large amounts (30 to 98%) of di-unsaturated wax esters; such waxes did not exceed 8% in the 'false neisseriae' or Moraxella spp. Waxes of strains belonging to the psychrophilic, oxidase-positive group generally resembled those found in Moraxella. Wax esters with odd numbers of carbon atoms were abundant in M. lacunata (29%), M. atlantae (15%) and in the psychorophilic group (19 to 28%); long-chain esters (C40 or above) were characteristic of M. atlantae (30%) and one strain of M. osloensis (26%).

Chromatography, Gas↗
PubMed1977

Oxidase positive rods from cases of suspected gonorrhoea. A comparison of conventional, gas chromatographic and genetic methods of identification.

Genito-urethral specimens from 3260 women and 1170 men, with ailments suggestive of gonorrhoea, were examined for growth of oxidase positive rodshaped bacteria, as well as of gonococci. Moraxella osloensis was identified in 26 cases (0.64 per cent of women and 0.43 per cent of men). Three patients harboured phenylalanine negative (or weakly reacting) and tryptophan deaminase negative M. phenylpyrouvica and, in three cases, a Flavobacterium species was detected. Among six oropharyngeal specimens from patients suspected of gonorrhoea, two yielded growth of oxidase positive rods, Kingella kingae and Neisseria elongata, respectively, N. gonorrhoeae was isolated from 537 patients, i.e., 12.1 per cent of all cases. The isolates of oxidase positive rods were in most cases completely identified by streptomycin resistance transformation. On this basis, the diagnostic reliability of some morphological and cultural-biochemical tests and gas chromatography was examined. Gas chromatographic analysis of fatty acid and alcohol composition of whole cells proved distinctive of species defined genetically, irrespective of confusing behaviour of some strains in other tests.

Bacteriological Techniques↗
PubMed1976

Cellular monosaccharide patterns of Neisseriaceae.

Sixty-four strains of Neisseria, Moraxella, and Acinetobacter were screened for cellular monosaccharides by gas-liquid chromatography and other chromatographic techniques. The four sugars ribose, glucose, glucosamine, and 2-keto-3-deoxyoctonate (KDO) were detected in all strains. Heptose was detected only in "true neisseriae" (Neisseria gonorrhoeae, N. meningitidis, N. sicca, N. cinerea, N. flavescens, and N. elongata) and in the tentaively named species Moraxella urethralis. Some marked interspecies dissimilarities within groups were revealed. Thus, N. ovis and M. atlantae were characterized by the presence of mannose. Intraspecies differences were also encountered. N. meningitidis strains of serogroups B and C were distinguished from strains of serogroup A by their sialic acid content. This sugar was also detected in two out of three examined strains of M. nonliquefaciens. In Acinetobacter, heterogeneity of monosaccharide patterns was rather pronounced. The results show the applicability of gas chromatographic "monosaccharide" profiles fo whole cells or extracted carbohydrate in bacterial classification and identification, including differentiation at the subspecies level. In addition, such profiles may be useful for monitoring during purification of cellular polysaccharides.

Acinetobacter↗
PubMed1975

Gas chromatography of bacterial whole cell methanolysates. VII. Fatty acid composition of Acinetobacter in relation to the taxonomy of Neisseriaceae.

The cellular fatty acids of seventeen Acinetobacter strains were determined. Most acids identified were previously found in neisseriae and moraxellae. Specific for Acinetobacter was 2-hydroxydodecanoid acid and a few minor unidentified components. The fatty acid data were analysed by numerical methods and compared with previous results obtained for neisseriae and moraxellae. The findings were consistent with genetic evidence for some affinities of genus Acinetobacter to genus Moraxella and "false neisseriae". Occasionally, a high resemblance in fatty acid pattern was demonstrated between a Moraxella strain and certain strains of Acinetobacter, and also between an Acinetobacter strain and certain "true neisseriae". Still, the acinetobacters constituted one single cluster separated from the other genera of Neisseriaceae.

Acinetobacter↗
PubMedDate not supplied

Civilian external peer review: lessons learned.

Since 1986, the Department of Defense has contracted with an outside agency for Civilian External Peer Review (CEPR). From January, 1986 to December, 1988, records indicate that approximately 350,000 discharges from Army hospitals have been included in the study. Initially, the small number of positive findings prevented generalizations; as the total number of cases screened has increased, several valuable lessons have been learned suggesting avenues for improving care. Additionally, a great deal has been elucidated concerning the process of large-scale clinical peer review.

Evaluation Studies as Topic↗