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Biomedical subjects

E Janssen

Publications and source records attributed to E Janssen.

At least 37 records · Page 2Linked to original sources
PubMed1994

Validation of a psychophysiological waking erectile assessment (WEA) for the diagnosis of male erectile disorder.

OBJECTIVE: The aim of the present study was to evaluate a psychophysiological waking erectile assessment (WEA). WEA was designed to elicit penile responses using visual and vibrotactile stimuli and cognitive tasks (distraction and monitoring of erections). METHODS: One hundred consecutive patients with erectile dysfunction and a control group of 50 sexually functional males were examined. Clinical decision analysis, including a receiver operating characteristic (ROC) analysis, was used to determine the accuracy of WEA in predicting independent diagnostic classifications. RESULTS: Fifty percent of the patients were independently diagnosed as having pure psychogenic impotence. In 45 percent of the patients an organic factor was found. Discrimination was best in three (out of 7) WEA conditions in which film was combined with vibration. This combination of conditions resulted in a test sensitivity of 81 percent, and predictive values positive (the probability of "no organic involvement" given a penile response greater than 12 mm) ranging from 66 to 95 percent. About one third of the patients with pure psychogenic erectile dysfunction had an average response of more than 30 mm to the three conditions combining vibration and film. None of the patients with organic involvement exceeded this 30 mm criterion. Thus, the predictive value positive reached its maximum of 100 percent. CONCLUSIONS: The results suggest that WEA is an appropriate initial screening procedure, and that it is of particular value in the detection of psychogenic cases.

Decision Support Techniques↗
PubMed1994

A self psychological approach to treating the mentally ill, chemical abusing and addicted (MICAA) patient.

There is a growing population of mentally ill, chemical abusing and addicted (MICAA) patients being admitted to inpatient psychiatric units. This article proposes a self psychological approach for this population and discusses how this model addresses the underlying intrapersonal and interpersonal reasons for chemical abuse. A synopsis of self psychology is presented and applied to both addiction and MICAA patients. A self psychological treatment approach is reviewed, including specific nursing interventions.

Diagnosis, Dual (Psychiatry)↗
PubMed1994

Visual stimulation facilitates penile responses to vibration in men with and without erectile disorder.

This study compared reflexogenic and psychogenic penile responses in men with and without erectile disorder. It was hypothesized that men with psychogenic erectile dysfunction respond minimally to vibrotactile stimulation. An enhancement of penile responses was expected when vibration was combined with erotic film. Patients were 50 men with psychogenic erectile dysfunction, 45 men with organic erectile dysfunction, and 50 sexually functional men. The combination of film and vibration resulted in stronger penile responses than the stimuli presented separately. Men with psychogenic erectile dysfunction and sexually functional men did not differ in responses to film and film-and-vibration conditions. As predicted, responses of the 2 groups were different in the vibration condition. Interpretations are provided in terms of attention and appraisal. The findings are relevant to the development of psychophysiological diagnostic procedures.

Adult↗
PubMed1986

B and T lymphocyte mitogenic properties in a snake venom.

From the crude snake venom of Agkistrodon rhodostoma two mitogenic principles could be detected: A potent T-lymphocyte stimulating anti-beta-D-galactose specific lectin with a molecular mass of about 18 kD and a very strong B-lymphocyte mitogenic factor. Whereas the lectin could be purified by affinity chromatography and characterized as a cell agglutinating, but not precipitating lectin, the highly active B-lymphocyte mitogen could be separated but not characterized. Both factors are much stronger than the mitogens known so far.

Animals↗
PubMed1985

Group B streptococcus type II antisera have anti-galactan specificities.

It has been confirmed by immunochemical methods that group B streptococcal capsular polysaccharides of type II contain terminal non-reducing beta-(1-6)-D-galactosyl groups. This immunodominant structure is detected by immune antisera from rabbits. As beta-(1-6)-D-galactosyl residues are the predominant end groups of a number of plant and animal galactans, anti-type II streptococcus antisera can also be used as anti-galactans. On the other side, different anti-galactans from various origins (lectins, myeloma proteins) can be applied to identify group B streptococcal type II strains. In addition, it is shown that other group B streptococcal types can also be recognized by different lectins.

Agglutination Tests↗
PubMed1985

Jackfruit (Artocarpus integrifolia) and the Agaricus mushroom lectin fit also to the so-called peanut receptor.

The lectin from jackfruit (Artocarpus integrifolia) reacts with the peanut-specific lectin receptor represented by the T-disaccharide, which is also part of the Thomsen (= T)-Friedenreich antigen, namely O-beta-D-galactosyl-(1----3)-N-acetyl-D-galactosamine. The difference between the jackfruit lectin and peanut lectin has been shown to be mainly due to the further linkage of the T-disaccharide: Whereas the peanut lectin reacts with both anomeric forms, jackfruit lectin and the closely - with respect to its specificity - related Agaricus bisporus mushroom lectin react predominantly with the alpha-linked T-disaccharide, so that they cannot detect the lipid-linked T-disaccharide, as the peanut lectin does.

Antigens, Tumor-Associated, Carbohydrate↗
PubMed1984

[Determination of mercury, lead and cadmium in fish from the Rhine and Main using flameless atomic absorption].

From 1976 until 1982 854 fishes from the Rhine and in 1983 43 fishes from the Main-river were analysed for mercury, from 1980 until 1982 212 fishes from the Rhine and in 1983 43 fishes from the Main-river were analysed for lead and cadmium. The analytical behaviour of these elements with special emphasis on the digestion is described. The results demonstrate that the contamination of the fishes from the Rhine by mercury has decreased within the period. This becomes also evident by the decreasing number of samples which exceed the tolerances. For lead and cadmium no trend can be deduced from the results. The contamination, however is appreciably higher than in unpolluted waters. Cadmium and lead seem to increase in concentration during the course of the river as demonstrated by a comparison of results from different stretches of this river. BGA-guide values (Federal Office of Health) were exceeded in some samples.

Animals↗
PubMed1984

Acid-base balance during repeated bouts of exercise: influence of HCO3.

Ten men and one woman were studied before, during, and following five 1-min cycling bouts (100% VO2 max) 1 h after consuming either 0.2 g/kg B.W. of NaHCO3 or a placebo drink (NaCl). The fifth exercise bout was performed until exhaustion and the time recorded to judge the effects of the NaHCO3 drink on performance. Blood samples taken from a forearm vein revealed that the intake of NaHCO3 increased resting pH (7.34 to 7.41), HCO3 (27.5 to 31.0 mM), and base excess (1.1 to 4.6 mM). During and following the exercise, blood pH and HCO3 were always higher (P less than 0.05) in the NaHCO3 than placebo trial. Performance times during the fifth cycling bout averaged 113.5 (SE +/- 12.4) and 160.8 (SE +/- 19.1) s in the NaCl and NaHCO3 trials, respectively. This 42% difference between the means was significant at the 0.01 level. The blood hydrogen ion to lactic acid ratios (nM/mM) measured between each exercise bout and during recovery from the exhaustive fifth bout suggests that the enhanced performance during the NaHCO3 trial was the result of greater buffer capacity. Thigh muscle (vastus lateralis) pH measured immediately before the fifth cycling bout in four of the subjects revealed that the working muscles were less acid in the NaHCO3 trial (pH = 6.81) than during the NaCl treatment (pH = 6.73). Thus, the alkalizing influence of oral HCO3 supports the concept that the hydrogen ion concentration in blood and muscle has a direct influence on performance during repeated, supramaximal exercise.

Acid-Base Equilibrium↗
PubMed1982

Two different anti-galactan lectins in eel serum.

Two different anti-galactan lectins from eel serum (Anguilla anguilla) were isolated by affinity chromatography on fucogel and on 1-acyl-2-(9-carboxy)nonyl-glycero-3-phosphocholine coupled to Aminohexyl-Sepharose. The fucolectin and the CRP-analogue protein thus purified directly from the eel serum were characterized by SDS-gradient gel electrophoresis, immunoelectrophoresis, and by agar gel diffusion tests. The CRP-analogue protein with a molecular weight of 24.800 Daltons was present in a high concentration. Carbohydrates are absent in both the proteins, showing that the fucolectin and the CRP analogue do not belong to the immunoglobulin/antibody group of serum proteins.

Animals↗
PubMed1982

Limitations of concentration measurements for evaluation of endocrine status of exercising women.

The impact of changes in metabolic clearance rates (MCR), biologic activity of sex-steroids, steroid-protein interaction and steroid-binding properties of plasma proteins in relation to physical exercise are reviewed. The MCR of estradiol is decreased during physical exercise but this phenomenon is only partly explained by a decreased hepatic blood flow. Changes in concentration of high-affinity steroid binding proteins (testosterone-estradiol-binding globulin, TeBG) affect predominantly the amount of free testosterone (T) and its MCR. During exercise both the lower sex-steroids binding affinities of plasma proteins due to increased body temperature and the increased concentration of these hormones cause a shift towards an increased free concentration. Due to competitive interactions between androgens and estrogens for the binding site of TeBG the free concentration of estrogens will probably increase more than expected. The physiological meaning of these changes are discussed.

Adolescent↗
PubMed1980

A quantitative study of free amino acids in eccrine sweat collected from the forearms of healthy young men during sauna bathing.

Using ion-exchange column chromatography we measured the free amino acids in eccrine sweat collected from the forearms of 20 healthy young men at intervals of 15, 30, and 45 min during sauna bathing. In comparison with the amino acid concentrations determined after 15 min of sweating (100%) a significant and uniform decrease occurred in the concentrations of all amino acids examined after a further 15 min (65%), with a further decrease after another 15 min (45%). These results indicate that either the mechanism of excretion of amino acids in the secretory cells of the eccrine sweat glands, or the reabsorption of amino acids by the ductular cells, or the combination of both mechanisms are similar for all the amino acids examined here.

Adult↗
PubMed1980

Isolation, characterization and implications of anti-TF (Thomsen-Friedenreich) agglutinins from different sources.

Anti-TF agglutinins from peanut (Arachis hypogaea) and from vertebrate sera of different species have been successfully isolated by affinity chromatography on acid-activated Sepharose 4 B. The proteins were characterized by immunoelectrophoresis, polyacrylamide gel electrophoresis in the presence of SDS and with respect to their carbohydrate binding specificities. Anti-TF substances from sera showed one precipitin arc in immunoelectrophoresis, but quantitative immunoprecipitation revealed our human anti-TF to be a mixture of the three Ig-classes IgG, IgA and IgM. This finding was confirmed on SDS gel electrophoresis, where high molecular weight aggregates were found before reduction. Hemagglutination inhibition revealed that all isolated anti-TF compounds exhibit an exceptionally high affinity for the immunodominant group of the TF-antigen, namely the beta-D-galactosyl-(1 leads to 3)-N-acetyl-D-galactosamine disaccharide. On examination of formalin-fixed and neuraminidase treated tissue sections (kidney, mammary gland), fluorescein-labelled anti-TF from horse serum showed a virtually identical pattern when compared with fluorescein labelled peanut lectin. Likewise isolated IgA-class myeloma J 539, which shows specificity against beta-(1 leads to 6)-galactans, only bound to the appropriate Gal-beta-(1 leads to 6)-Gal structures, such as those found on bovine lung or the albumin gland of Helix pomatia. Rabbit anti-VCN (Vibrio cholerae neuraminidase) activity could be selectively abolished by beta-galactosyl-containing inhibitors, whereas papain F(ab) fragments from rabbit anti-VCN immunoglobulin did not compete with anti-TF for binding sites on VCN-treated human red cells. Anti-TF, on the other hand, did not compete with anti-VCN for active VCN.

Agglutinins↗