Production of cardio-active substances by plant tissue cultures and their screening for cardiovascular activity.
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Alkaloids↗
Biomedical subjects
E J Staba
Publications and source records attributed to E J Staba.
Visnagin: biosynthesis and isolation from Ammi visnagi suspension cultures.
During an examination of Ammi visnaga Lam. suspension cultures for the biosynthesis of furanochromones and related medicinal compounds, visnagin was isolated in crystalline form and identified. Thus, certain medicinally important secondary plant metabolites may be produced in appreciable amounts by plant tissue cultures.
Chemical Phenomena↗
Allergens from short ragweed leaf tissue cultures.
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Allergens↗
American and Korean ginseng tissue cultures: growth, chemical analysis, and plantlet production.
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Cell Division↗
American ginseng. II. Analysis of ginsenosides and their sapogenins in biological fluids.
A gas-liquid chromatography (tlc) method was developed to assay individual ginsenosides and sapogenins in rabbit plasma and urine samples. A flavonoid, panasenoside, and a sterol, stigmasterol, were used as internal standards for ginsenosides and their sapogenins, respectively. Linear relationships of peak height ratio to weight ratio were obtained for ginsenosides (A1, 20-350 microgram; A2, 20-400 microgram; B2, 20-300 microgram; C, 20-500 microgram), and sapogenins (panaxadiol or panaxatriol, 10-200 microgram) in 0.1 ml of the silylation mixture. The glc assay method developed was sensitive to 0.2 microgram of ginsenosides and 0.1 microgram of sapogenins.
Animals↗
Steroids and an artifact from Dioscorea deltoidea tissue cultures.
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Culture Techniques↗
Suitability of antidigoxin antiserum for digoxin in plant extracts.
Antiserum specificity for digoxin was demonstrated by means of three tests: cross-reactivities test, dilutions test and thin-layer chromatography test. The cross-reactivities test examined quantitatively the cross-reactivities with specific compounds and provided an explanation for the basis of the dilutions test. The dilutions test was used to demonstrate the suitability or unsuitability of the antiserum for use in the radioimmunoassay of digoxin in a crude biological sample. The tlc test examined for antiserum cross-reactivity with digoxin and related compounds in a crude extract.
Animals↗