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Biomedical subjects

E J Hart

Publications and source records attributed to E J Hart.

17 recordsLinked to original sources
PubMed1987

Neurobehavioral outcome after closed head injury in childhood and adolescence.

Hospital records of 53 children and adolescents, aged 18 years or less, with closed head injury were reviewed for information on long-term outcome. Computed tomographic scans were used to divide the patients into clinicopathologic groups. Within these groups, duration of coma was the major index of severity. Outcome was assessed using the Glasgow Outcome Scale and by evaluating social behavior, school performance, and vocational functioning. Patients with diffuse injury plus focal lesions fared worse than those with diffuse injury only. Coma lasting more than one month led to a poorer outcome in both groups. Many individuals had limiting emotional disturbances, which may have resulted from disruption of frontal systems modulating arousal and social behavior.

Adolescent↗
PubMed1987

Acquired aphasia in childhood: clinical and CT investigations.

We studied eight children with acquired aphasia. All had left hemisphere lesions. In most, the correlation between the CT lesion site and the resulting aphasic syndrome duplicated an anatomic-clinical correlation described in adults. Rapid recovery of language fluency distinguished the children from reported adults. Late follow-up indicated poor scholastic achievements, reflecting an acquired handicap in new learning. Anatomic-clinical correlates and recovery patterns suggest that brain organization for language is similar but not identical in children and adults.

Adolescent↗
PubMed1976

Isolation and characterization of a bacteriophage specific for Neisseria perflava.

Six isolates from normal throat samples have been shown to contain phage active against Neisseria perflava. The phage isolates were similar in terms of host range, latent period, burst size, antigenic properties, morphology, and nucleic acid content. Neutralization studies with antisera demonstrated that the isolates exhibited a very high degree of serological relatedness. These results taken together suggested that the isolates represented a single strain of bacteriophage. This phage, which we have designated NP-1, exhibited a high degree of host specificity, attacking only one of the several strains of N. perflava tested and none of the other species tested. One-step growth experiments yielded minimum latent periods of approximately 35 min; average burst sizes varied from 34 to 63 plaque-forming units per cell. Electron micrographs revealed particles with heads averaging 75 nm in diameter and tails averaging 300 nm in length and 18 nm in diameter. The phage contained double-stranded DNA with a guanine plus cytosine content of 38%.

Bacteriophages↗
PubMed1975

Conditions required for the attainment of colony-type stability of Neisseria gonorrhoeae in liquid culture.

Colony-type morphology in Neisseria gonorrhoeae is associated with virulence, transformability, and the presence or absence of pili. A reliable method for achieving large populations of cells that are relatively stable with respect to colony type would be valuable, for example, in studies of virulence or for the isolation of pilus-specific phages. Previously described methods designed to achieve type stability in liquid culture were inadequate for a variety of reasons, including their low final cell yields and/or their requirements for prolonged incubation. The success of the procedure described in this communication depends upon the use of an overnight plate harvest to insure a relatively large and stable inoculum for the liquid medium. Yields of as high as 10(10) colony-forming units/ml are routinely obtained after 4 to 5 h of incubation. Such cultures exhibit a colonial-type stability of 85 to 95% with respect to the original colonial type used for inoculation of the start plate.

Bacteriological Techniques↗