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Biomedical subjects

E I Kvasnikov

Publications and source records attributed to E I Kvasnikov.

At least 37 records · Page 2Linked to original sources

[Effect of carbon source and cultivation temperature on growth limitation of Candida tropicalis by biotin].

The effect of biotin on the specific growth rate of Candida tropicalis was studied on media with various sources of carbon nutrition at temperatures of 29 and 39degreesC. The yeast was found to require 3--5 times less biotin on the medium with paraffin than on the medium with glucose and hexodecane, and 3--4 times more biotin on the same substrate with an increase in temperature. The growth rate increased by 36--40% on liquid paraffins containing 0.5 mcg/litre of biotin at 29degreesC and 1.5--2.0 mcg/litre of biotin at 39degreesC. The saturation constant of biotin increased from 0.033 to 0.063 mcg/litre on the medium with paraffin, and more by an order on the medium with glucose and hexadecane, at a rise of growth temperature from 29 to 39degreesC.

Alkanes↗

[Chemotaxonomic markers of several coryneform bacteria and the "Rhodochrous" group].

Chemotaxonomic characters (the monosaccharide composition and diaminopimelic acid (DAP) of cell hydrolysates, the type of lipid LCN-A) were studied with bacteria of the "rhodochrous" complex and the Arthrobacter genus isolated from natural substrates as well as with collection strains of the genera Arthrobacter, Brevibacterium and Corynebacterium. If arabinose, galactose and meso-DAP were present in cell hydrolysates of these bacteria, they always contained lipid LCN-A. Bacteria of the "rhodochrous" complex can be differentiated from Corynebacterium sensu stricto by location of lipid LCN-A spots on chromatograms, and from the genera Brevibacterium (B. linens) and Arthrobacter, by the presence of arabinose, meso-DAP and lipid LCN-A in the cells. The representatives of two latter genera do not contain arabinose and lipid LCN-A in their cells.

Actinomycetales↗

[Distribution of Rhodosporidium diobovatum yeasts in soil and on plants].

As was found by the hybridization technique, 43 strains among 92 studied strains of the yeast Rhodotorula glutinis belong to Rhodosporidium diobovatum Newell et Hunter (Ustilaginaceae, Basidiomycetes). The strains of Rhodosporidium diobovatum were found in oil-bearing soils, rhizosphere, and phyllosphere of cultivated and wild plants.

Basidiomycota↗

[Yeast flora of plant rhizosphere and phyllosphere].

The species belonging to the genera Cryptococcus and Hansenula with saturnian spores predominate in the rhizosphere of agricultural plants. The sporiferous strains of Debaryomyces, Hanseniaspora apiculata, Metschnikowia pulcherrima, and asporogenic Candida krusei and Trichosporon cutaneum prevail in the rhizosphere of wild plants. Candida krusei and Trichosporon cutaneum prevail in the rhizosphere of wild plants. The cultures of Rhodotorula, Candida krusei and Metschnikowia pulcherrima are typical of the phyllosphere of both cultural and wild plants. The phyllosphere of cultural plants contains also the asporogenic strains of Cryptococcus, Candida tropicalis, Trichosporon pullulans, Tr. cutaneum, and Hansenula, while Hanseniaspora apiculata and Saccharomyces cerevisiae predominate in the phyllosphere of wild plants. The yeast flora of the majority of studied plants is diverse and comprises 10--20 species (in cabbage, potato, linden, aspen, and pear trees). The rhizophere and phyllosphere of some plants contain only 2 to 4 yeast species (onion, hop, wild apple).

Plants↗

[Structure of Candida tropicalis K-41 thermotolerant yeasts at various growing temperatures on a mineral medium with hydrocarbons].

In the C. tropicalis K-41 cells fixed by glutaraldehyde and osmium most membranes consisted of two electron-transparent and one electron-dense layer. Morphologo-structural peculiarities of the cell fraction grown at 39 degrees testify to a certain disconnection of energetic and constructive metabolism, inhibition of synthetic processes. Cell division was more active at 29 degrees.

Alkanes↗

[Lactic bacteria in the digestive tract of poultry].

Lactic bacteria predominate in the microflora of the digestive tract of chicken and turkey. They are represented mainly by Lactobacillus acidophilus, L. salivarius, L. fermentum and L. buchneri. Streptococcus faecium is always isolated. L. ruminis, L. vitulinus, L. delbrueckii, L. coryniformis and L. viridescens were found in this ecological niche for the first time. S. faecium and S. faecalis prevail in the digestive tract of geese and ducks, while lactobacilli are detected in a lesser amount and are represented mainly by L. plantarum. L. salivarius cells isolated from the digestive tract of poultry are highly polymorphous. Most of the lactic acid bacteria found in the digestive tract of poultry can grow at 45-50 degrees C whatever is the species they belong to.

Animals↗