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E I Kovalenko

Publications and source records attributed to E I Kovalenko.

12 recordsLinked to original sources
PubMed2006

[The long-term results of the dynamic observation of patients with adrenal gland adenomas].

The authors demonstrate the results of many years of observing patients with hormone-inactive adrenal gland adenomas. The subjects were 80 patients observed during 8 months to 12 years. The study shows that slow growth is generally typical of these adenomas. In 15% of patients an average growth of 1 mm/year was noted; this growth did not exceed 10 mm throughout the whole period of observation. The researchers also observed changes in the clinical symptoms and hormonal parameters over time. The study demonstrates that long existence of adenomas do not aggravate the clinical course of arterial hypertension and diabetes mellitus, and do not express any hormonal activity. The study established more precise indications to dynamic observation and its algorithm.

Adrenocortical Adenoma↗
PubMed2005

[Disputable points and negative tendencies in diagnosis and surgical treatment of accidentally revealed adrenal tumors].

Two main problems are discussed in detail: differential diagnosis of adrenal tumors before surgery, and policy of treatment of these patients. Indications for fine needle biopsy are determined, based on personal and world experience reasoned indications to surgical treatment and follow-up are formulated. Some negative tendencies in adrenal surgery are also discussed.

Adrenal Gland Neoplasms↗
PubMed2002

[Prospects of computer tomography in diagnosing adrenal tumors].

Computed tomography (CT) is one of main topical methods of adrenal tumors diagnosis. Medical histories of 68 patients treated from 1995 to 2000 for various adrenal tumors were analyzed. CT was used in all the patients. These data were compared with results of morphological study. It is demonstrated that CT has high sensitivity in diagnosis of adrenal tumors (96.55% cases). It permits to predict the type of tumor with high probability. The degree of tumor size mistake made at CT was estimated. Mean coefficients to assess true sizes of tumors before surgery were calculated. This is important for choice of treatment policy in hormonal-unactive adrenal tumors.

Adrenal Gland Neoplasms↗
PubMed1998

[Incorporation of neoglycolipids into K562 cells. Model for the study of carbohydrate dependent lysis of target cells by natural killer cells].

A model system was developed to study the role of cell surface oligosaccharides in cytotoxicity mediated by natural killer (NK) cells. Polymeric glycoconjugates Glyc-PAA-PE and Glyc-PAA(Flu)-PE (where Glyc is the 3-aminopropyl Le(x) residue, PAA is the poly(N-2-hydroxyethylacrylamide) matrix, Flu is the residue of a fluorescein derivative, and PE is the phosphatidylethanolamine residue) were synthesized, and their association with the membranes of living K562 cells was studied.

Cell Membrane↗
PubMed1985

A new statistical method for identifying interconnections between neuronal network elements.

A new method is proposed to analyse dependencies in point processes, which takes into account specific character of neuronal activity. Simulation modelling of neuronal network revealed that the estimated weight of connection depends monotonically on the value of the model synaptic strength. In contrast to the crosscorrelation, the method allows for nonlinear interconnections and does not require point processes to be stationary and samples to be large. Examples are presented of the method's application to neurophysiological data analysis.

Animals↗
PubMed1981

[Types of spike activity and nature of neuronal interrelations in the rat neostriatum].

Statistical methods were used to study types of background activity (BA) of neostriatum neurons. Dependence of the crosscorrelation function on the BA type and on a distance between the neurons was examined in acute experiments on rats (one group was immobilized with d-tubocurarin, the other was under nembutal anesthesia). It is shown that BA according to the shape of the autocorrelation and crosscorrelation functions can be classified into five types (I.1, I.2, I.3, II.1, II.2). Pronounced differences in the crosscorrelation function were found between the neurons with grouped BA (I.1, I.2) and the neurons with unitary BA (II.1, II.2). Differences in the crosscorrelation function between close and distant (300-500) microns) neurons were also found.

Action Potentials↗
PubMedDate not supplied

[Modification of the target cell surface with lipophilic glycoconjugates and interaction of the modified cells with natural killer cells].

An experimental model system involving the modification of carbohydrate composition of the target cell surface with neoglycolipids was developed for studying the role of surface carbohydrates of target cells in the NK-cell-mediated cytotoxicity. The polymeric glycoconjugates of the Glyc-PAA-PEA and Glyc-PAA(Flu)-PEA types (where Glyc was an oligosaccharide residue, PAA poly(acrylamide) polymer, and PEA the phosphatidylethanolamine residue, and Flu fluorescein residue) capable of incorporation into the cell membrane were synthesized. The optimum structures of neoglycoconjugates and conditions for their incorporation into K562 and Raji cell lines, which differ in their sensitivity to the NK-cell-mediated lysis were selected. The mechanism of association of glycoconjugates with the plasma cell membrane and the kinetics of their elimination from the cell surface were investigated using the fluorescent-labeled Glyc-PAA(Flu)-PEA derivatives. The spatial accessibility of the carbohydrate ligands for the interaction with human NK cells was demonstrated. The target cells modified with the Le(x) trisaccharide were shown to be more sensitive to the cytotoxic effect of human NK cells than the intact cells. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 3; see also http://www.maik.ru.

Cell Line↗