Search PubMed⌕ Search

Biomedical subjects

E Hultman

Publications and source records attributed to E Hultman.

245 records · Page 14Linked to original sources
PubMed1965

Sulfasymazine.

Explore the source record for details and available documents.

Escherichia coli Infections↗
PubMedDate not supplied

A luminometric method for the determination of ATP and phosphocreatine in single human skeletal muscle fibres.

A sensitive method for the analysis of ATP and phosphocreatine (PCr) in single human skeletal muscle fibres is described. Muscle tissue was freeze-dried and single fibres were dissected free with the aid of low-power microscopy. The fibres were then extracted in trichloroacetic acid and neutralized with KHCO3. The assay is based on the continuous monitoring of light produced as a result of ATP degradation in the firefly luciferase reaction. PCr is measured as the amount of ATP formed in the creatine kinase reaction. The coefficient of variation was less than 4% for both ATP and PCr determination. The amount of tissue required for the assay is approximately 0.5 microgram (dry weight). The assay showed good agreement with spectrophotometric and high-performance liquid chromatographic (HPLC) measurements made upon extracts of whole muscle tissue.

Adenosine Triphosphate↗
PubMedDate not supplied

Preliminary studies of energy-rich phosphagens in muscle from severely ill patients.

Preliminary studies on muscle tissue metabolism were made in a series of 18 patients treated in an intensive care unit. In acutely ill patients with circulatory or respiratory insufficiency, there was an increase in muscle lactate content, a decrease in the phosphorylcreatine stores as well as decreased in adenosine triphosphate (ATP) and total adenine (TA) contents. These findings could partly be explained by a relative hypoxia in the muscle but acute hypoxia alone would not account for the decrease in ATP or TA. These changes in the adenylate pool were still more pronounced in patients with prolonged diseases. In this series the ATP content was only 50% of the normal, despite normal lactate content. The reason for the low adenine nucleotide level in muscle tissue is thought to be due primarily to an increased formation and deamination of adenosine monophosphate during hypoxia in combination with a decreased rate of purine synthesis in the liver and/or a decreased capacity for "purine salvage" in the muscle. This itself might, in turn, be mediated by a low energy state in muscle or liver or be due to other metabolic disturbances or tissue damage. It was found that prolonged immobilization without metabolic disturbances did not change the TA content in muscle, while short-lasting severe metabolic acidosis decreased the TA content. A correction of the metabolic disturbance immediately increased the TA content in muscle. A low energy charge potential was found in patients with prolonged diseases, possibly being the cellular expression for the concept of the post-traumatic catabolic state.

Adenine↗
PubMedDate not supplied

Determination of water and electrolytes in muscle biopsies in the nutritional assessment of clinical disorders.

Nutritional inadequacies profoundly affect the content and distribution of water and electrolytes in muscle tissue. However, most changes in tissue water and electrolytes are nonspecific and reflect the degree of sickness; they cannot be used for evaluation of malnutrition, except to indicate whether there is a loss or excess of intracellular ions, such as potassium and magnesium. In patients without disturbances in potassium metabolism, muscle cell potassium and protein are strongly correlated, but this relationship does not hold true in patients with potassium depletion. Accumulation and loss of potassium occur in parallel with changes in muscle glycogen, independent of other muscle constituents, eg, magnesium. Potassium depletion and excess, as well as changes in muscle glycogen, must be considered when evaluating the relation of lean body mass and total body protein content to total body potassium.

Biopsy↗
PubMedDate not supplied

Experimental acute sinusitis in rabbits. Energy metabolism in sinus mucosa and secretion.

In previous studies, sinus secretions have been analysed concerning pO2, pCO2 and pH. In this study the energy metabolism in the maxillary sinus mucosa and secretion was studied in 33 New Zealand White rabbits. In freeze-dried samples, glucose and lactate were analysed by enzymatic assays and ATP by HPLC. In the purulent sinusitis, lactate concentration in the mucosa was 5.67 mmol X kg-1 w.w. and significantly higher than in the control sinus. Also in the non-purulent sinusitis, lactate was increased in the mucosa. The lactate concentrations in the secretion were 9.8 and 8.4 mmol X kg-1 w.w. in purulent and non-purulent secretions respectively and are probably the result of a diffusion from the mucosal cells and of leukocyte metabolism. A reduced ATP content, with increases in ADP and AMP, in the sinus mucosa of the purulent sinusitis suggests a relative energy depletion which could result in impaired epithelial function.

Adenine Nucleotides↗