Search PubMed⌕ Search

Biomedical subjects

E Housset

Publications and source records attributed to E Housset.

At least 109 records · Page 6Linked to original sources
PubMed1977

[Electrophoretic mobility of antithrombin III in an agarose gel with heparin. (author's transl)].

The electrophoretic mobility of several forms of antithrombin III in an agarose gel has been compared with the mobility in a gel containing heparin. When a serum was studied, three different compounds were observed. The inactige antithrombin III with higher molecular size, separated by gel filtration, was found to be homogenous even if there was heparin in the gel. A purified antithrombin III, prepared by affinity chromatography, contained an immunoreactive material of higher molecular size which has no activity and a higher mobility in agarose gel. When heparin is incorporated in the agarose plate, the electrophoretic mobility of this polymerized antithrombin III is not modified.

Antithrombins↗
PubMed1977

Immunochemical study on serum proteins in systemic sclerosis.

Forty one patients with systemic sclerosis were studied after separation into three groups according to Barnett's classification. A multi-dimensional statistical analysis eight serum proteins revealed a difference between control patients and patients with type I and type II scleroderma. Type I scleroderma was characterised by a rise in alpha 2 macroglobulin and in the C4 fraction of complement, whilst in type II scleroderma all the proteins studied were raised, with the exception of CO complement, which was normal, and transferrin which was markedly decreased.

Blood Proteins↗
PubMed1976

[Gonococcal septicemia. 1 case].

This paper describes a case of disseminated gonococcal infection, a rare disease in France. A 41 year-old woman was hospitalised with acute polyarthritis and characteristics skin lesions. Jaundice and liver function abnormalities are difficult to interpret due to a preexisting alcoholic cirrhosis. The possibility of an endocarditis is raised because of a systolic murmur heard at the base of the heart. Bacteriological identification of N. Gonorrhoeae is carried out in blood culture; it has also been recovered by scrapings of a cutaneous bullae by staining only. Therapy was instituted by daily intravenous penicillin G sodium 50 000 000 u. and intramuscular gentamicin 160 mg for 45 days. There resulted good clinical and bacteriological response. The elements of clinical and bacteriological diagnosis, as well as the therapy are discussed.

Acute Disease↗
PubMed1976

Breast milk jaundice: in vitro inhibition of rat liver bilirubin-uridine diphosphate glucuronyltransferase activity and Z protein-bromosulfophthalein binding by human breast milk.

Twenty-four samples of breast milk from nine mothers of infants suffering from breast milk jaundice were studied. Eight samples of milk from mothers of nonjaundiced infants, along with five formula milks enriched with polyunsaturated fatty acids, served as controls. Milks from mothers with jaundiced infants had no inhibitory effect when assayed immediately after thawing. However, after these milk samples were stores at 4 degrees, they strongly inhibited bilirubin conjugation (80.3% inhibition of uridine diphosphate glucuronyltransferase (UDPGT) activity) and bromosulfophthalein (BSP) binding to cytoplasmic Z protein (dye binding inhibited 82.1%). There was no effect on BSP binding to Y protein (see Table 1). Heating the milk to 56 degrees modified the results in the following manner; when the milk was heated immediately after thawing, no inhibitory effect was seen, even after storage for 96 hr. On the other hand, when the milk was first stored at 96 hr and then heated, it had the same inhibitory effects as the milks which were stored without heating. The present study shows that pathologic breast milk will inhibit BSP-Z protein binding only when stored under conditions that also cause the appearance of the capacity to inhibit bilirubin conjugation in vitro, as well as causing the liberation of nonesterified fatty acids. Thus, the appearance of this inhibitory capacity in vitro seems linked to the lipolytic activity particular to pathologic milks.

Animals↗
PubMed1976

Chromosomal breakage and scleroderma: studies in family members.

Chromosome studies were performed on 54 apparently healthy relatives of 29 scleroderma patients and on 40 controls. Increased chromosomal breakage is observed in 86 per cent of brothers and sisters and in 68 per cent of children of patients. If the findings in relatives are compared to those obtained in the 29 scleroderma patients of these families, the percentage of abnormal cells does not differ significantly between the two groups (25.8 per cent and 29.1 per cent respectively), and there is no difference for the frequencies of gaps and open breaks. However acentric fragments, "minutes" and morphologically abnormal chromosomes are significantly increased in patients as compared to their asymptomatic relatives with increased breakage. The distribution of breaks is found to be random. The presence of structural chromosome aberrations in relatives of scleroderma patients may have a special importance with regard to the concept of familial autoimmune disease.

Adult↗
PubMed1975

[Correlation between scleroderma and Sjogren's syndrome. Value of systematic biopsy of the labial salivary glands].

Thirty one patients with generalised scleroderma underwent labial biopsy. Sixteen showed a nodular infiltration of the accessory salivary glands indicative of Sjögren's syndrome. In 21, intralobular fibrosis was present. More common in those forms with extensive cutaneous involvement, collagenous fibrosis would appear to be a direct manifestation of scleroderma. The frequency of these abnormalities poses the problem of immunological competence with respect to lymphocytic labial infiltrates in generalised scleroderma.

Biopsy↗
PubMed1975

[Radiological and endoscopic study of the esophagus in 33 cases of generalized scleroderma].

The radiological and endoscopic picture of the oesophagus was investigated in 33 patients with generalised sclerodermia. Signs of involvement were noted in 26 subjects, whereas symptoms of such involvement had been observed in 13 only. Screening for oesophageal sclerodermia sites requires accurate assessment of oesophageal kinetics. Fibroscopic examination gives equally reliable evidence of involvement in the form of two distinctive signs: absence of peristaltic contractions in the lower half of the oesophagus and persistence of a "pool of water" in the organ for over 30 secs following injection of a few cc of water in the lying patient. Endoscopy frequently reveals concomitant peptic oesophagitis (10 cases). This is invisible radiologically. Endoscopy also permits accurate appraisal of the lesions associated with peptic stenosis. Fibroscopic examination of the oesophagus, in fact, could well be made a feature of the evaluation of all patients with actual or suspected generalised sclerodermia, on account of its aid to diagnosis and the early notice it gives of oesophageal complications.

Adult↗
PubMed1975

Discontinued thrombolytic treatment: initial biological results.

Changes in blood coagulation following the action of intermittent treatment with streptokinase are studied : 1) Fibrinogenemia remains at a concentration slightly higher than that obtained after the first 16 hour perfusion. 2) The thrombin time becomes considerably longer on the first day, then becomes shorter and remains slightly long throughout treatment. The theoretical interest of the rise in plasminogen levels during interruption of perfusions of streptokinase is discussed.

Humans↗
PubMed1975

[Intermittent thrombolytic treatment. Results during severe, chronic arterial diseases].

38 patients with severe chronic arteritis of the lower limbs were treated with streptokinase intermittently. All had been refused for surgical operation. One patient died, 4 others had early interruption of treatment. Eleven of the 38 patients had efficient thrombolysis confirmed by arteriography. The facts confirm the possibility of thrombolysis during chronic arterial disease. The fact that the aggravation was recent was favourable factor in prognosis. The eleven patients improved, had severe aggravation of symptomes for less than 2 months. Thus thrombolytic treatment has a place of choice in the treatment of severe arterial disease where surgery is impossible, or dangerous, owing to the uncertain state of the vascular bed below the lesion. Efficacious, it permits reconstructive surgery in cases where it had been at first refused. The use of intermittent treatment, apart from advantages of confort and cost, seems to increase the efficacy of treatment.

Adult↗
PubMed1975

[Radiological and endoscopic investigations of the esophagus in 33 cases of generalized scleroderma (author's transl)].

The esophagus was examined radiologically and endoscopically in 33 patients with generalized scleroderma. 13 patients complained of difficulties which suggested implication of the esophagus, scleroderma was found in the esophagus in 26. Radiological demarcation of location of the scleroderma required investigation of the esophageal peristalsis. Endoscopic examination permits an equally reliable assessment through the identification of characteristic signs: the lack of peristaltic contractions in the lower half of the esophagus, and the persistence of water in the esophagus for more than 30 seconds after injection of a few milliliters in the recumbent patient. Further, a peptic esophagitis can often be recognised endoscopically when it is not detectable radiologically (10 cases) and in cases of peptic stenosis (4 patients) exact location of the lesion is possible.

Adult↗
PubMed1975

Effect of trimethylcolchicinic acid on the synthesis and excretion of proteoglycans in tissue culture.

The action of trimethylcolchicinic acid on the synthesis and excretion of proteoglycans has been studied on the L cell strain. The incorporation of precursors has been measured, and proteoglycans produced in the culture medium have been extracted and their concentration determined. The mucopolysaccharide components have been studied by electrophoresis. Control cultures produce hyaluronic acid, dermatan sulfate and very low concentrations of chondroitin 4-sulphate or 6-sulphate. Cultures treated with trimethycolchicinic acid (4 mu g/ml) produce hyaluronic acid, very high concentrations of chondroitin 4-sulphate or 6-sulphate and only traces of dermatan sulphate. So, trimethylcolchicinic acid does not modify the synthesis of hyaluronic acid: it considerably increases the production of chondroitin 4-sulphate or 6-sulphate and inhibits the production of dermatan sulphate. Protein fraction of the proteoglycans is proportionally increased in treated cultures, but there is no marked difference between amino acid concentrations of proteoglycans extracted from control and treated cultures. A slight fall in the cystine concentrations was the only change in the amino acid content of proteoglycans extracted from treated cultures. A hypothesis to explain these results is discussed.

Amino Acids↗