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Biomedical subjects

E Hofmann

Publications and source records attributed to E Hofmann.

At least 289 records · Page 16Linked to original sources
PubMed1969

Molekulargewichtsbestimmungen durch polyacrylamidgelelektrophorese unter verwendung eines linearen gelgradienten.

A new method for molecular weight determination using polyacrylamidegel electrophoresis in a linear gel-concentration-gradient (3-20 degrees ) is described. Plotting the log of molecular weights of several standard proteins against distance of migration or against 3 log of rate of n-dgration reveal linear relationships in ranges of 50.000 - 200.000 or 100.000 - 400.000 Daltons respectively. On this basis, a simple method for molecular weight determination of proteins (accuracy +/- 5 degrees ) has been devised. The method can also be applied for an individual protein in protein mixtures using specific staining procedures.

Journal Article↗
PubMed1968

Some kinetic and molecular properties of yeast phosphofructokinase.

Yeast PFK had a sedimentation coefficient of 16.7 S both in the absence and in the presence of ATP, and did not dissociate even at very low protein concentrations. Sodium dodecyl-sulphate caused dissociation of the protein to sub-units of 3.2 S.The effects of pH on substrate affinities are described. In the presence of UTP, acting as non-inhibiting phosphate donor, the behaviour of the enzyme towards F-6-P was co-operative, with a Hill coefficient of 2.2.

Journal Article↗