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Biomedical subjects

E Hochuli

Publications and source records attributed to E Hochuli.

At least 73 records · Page 4Linked to original sources
PubMed1977

Dihydroxyacetone reductase from Mucor javanicus. 1. Isolation and properties.

An NADPH-dependent oxidoreductase has been extracted from the mycelium of the fungus Mucor Javanicus (Wehmer) and enriched 1000-fold with respect to the protein contained in the crude extract after centrifugation at 2600 X g. The molecular weight of the enzyme was estimated by gel filtration to be about 100 000; electrophoresis under dissociating conditions indicates four subunits of molecular weight about 28 000. Data on stability and activity of the enzyme as a function of pH and temperature are reported. From a kinetic study and product analysis of the reduction of the two enantiomeric trans-1-decalones and also from a kinetic study of the oxidation of the two diastereomeric pairs of trans-1-decalols it follows that the enzymes is an e-Si oxidoreductase (according to the nomenclature proposed by Dutler et al., Eur. J. Biochem. 22 [1971]203-212 and Prelog and Helmchen, Helv. Chim. Acta, 55 [1972] 2581-2598). This classification is amply confirmed by the kinetic behaviour of a large number of alicyclic substrates. Using (4-2HSi-labelled coenzyme to reduce (9S)-trans-1,4-decalindione, it was shown that the enzyme is HSi (= HS = HB)-stereospecific with respect to the coenzyme. It is demonstrated that the oxidoreductase from Mucor javanicus can be used for the preparation of optically pure chiral alcohols and ketones. In the following paper evidence is presented that the natural substrate of the enzyme is dihydroxyacetone.

Alcohol Oxidoreductases↗
PubMed1977

[Severe infections in our obstetric-gynecologic case material including nosocomial infections (1972--1976) (author's transl)].

A consideral by increased incidence of severe infections in 1975 provided the impetus for a critical analysis of such cases (n = 29) during the last four years. In the group of infections acquired outside the hospital setting, 13 were adnexal infections and 2 were amniotic infections. The group of true hospital infections (n = 14) consisted of 4 entoplastic cases, 7 cases with contaminated infusions and 3 cases of amniotic infections. A shift in the pathogenic spectrum in favor of gram-negative bacteria, particularly Klebsiella, Pseudomonas and anaerobes, was observed in those infections acquired within the hospital setting-- The mortality rate for the whole group was 10% (!).-- In addition to the usual hygienic measures used in hospitals to successfully combat endemic diseases resulting from severe nosocomial infections, a short-term perioperative prophylactic antibiotic, the advantages and disadvantages of which were mentioned, was integrated in the resistance dispositive. In severe adnexal inflammation (2 times in connection with an intrauterine device), the method of choice for surgical therapy was adnexectomy or, if necessary, hysterectomy and drainage. Septicemic diseases in connection with venipuncture material (entoplastic) can be avoided, for the most part, by carefully selecting the type and nature of the material as well as through preventive hygienic measures. The infusions contaminated exclusively by Klebsiella led to particularly serious infectious disease.

Adult↗
PubMed1977

The development of children born by vaginal or abdominal breech delivery (author's transl).

The early and late development of children born by the breech either by vaginal breech delivery or by caesarean Section is compared. The early and late morbidity of the two groups is discussed in detail. Considering the recently published data by Hagberg (6) theinterpretation of findings classified as secondary morbidity is very dificult. There are numerous especially antenatal factors which interfere with the interpretation. The conclusion is presented that it is a present not possible to make a definite statement on the value of vaginal or Caesarean Section delivery in breech presentation. It is still considered to be safe to do Caesarean Section delivery in breech presentation. It is still considered to be safe to do Caesarean Sections in breech presentations on an individualized basis.

Acidosis↗
PubMed1977

[Possibilities and limitations of obstetric intensive medicine: a cost-savings analysis (author's transl)].

With the increasing use of obstetrics intensive supervision methods, perinatal mortality could be decreased from greater than 2.5% to less than 1.0% between 1965-1975. Cases of clinically relevant acidosis in the umbilical artery (pH less than 7.10) declined from 2.41% to 0.51% between 1973-1975. On the basis of these results and the statistical data from Hagberg (14), it can be concluded that 1 child out of every 1.000 births can be preserved from a severe infantile cerebral paresis and 1 child from severe mental retardation via obstetric intensive supervision. The cost for obstetric intensive medicine per 1.000 births was 370.000 Swiss francs in 1975. As a result of the decline in cerebrally damaged and mentally retarded children, expenditures of between 1.3 to 1.9 million Swiss francs were avoided. This means a savings of 1 to 1.5 million Swiss francs.

Blood Chemical Analysis↗
PubMed1976

["Fetal monitoring" within the framework of actual obstetric monitoring].

The introduction of fetal monitoring and its use in high-risk patients was followed in the period 1971-1973 by the first-ever drop in perinatal mortality below 2%. Total monitoring brought about a further reduction in perinatal mortality to 0.95% (1974-1975). This decrease is statistically highly significant. It suggests that among all intensive care methods continuous fetal heart-rate monitoring plays the main contributory role in the early detection of fetal distress. We found that selection of high-risk patients was not sufficient to achieve a drastic reduction in the incidence of acidosis. Only with total fetal monitoring was it possible to effect a significant decrease (from 2.41% to 0.51%) in the number of infants with acidosis (pH less than 7.10).

Acidosis↗
PubMed1976

The effect of modern intensive monitoring in obstetrics on infant mortality and the incidence of hypoxia and acidosis.

UNLABELLED: We consider intensive monitoring to be fetal monitoring during labor and in the newborn period of all births using the most efficient methods. During the last ten years we have sequentially used the following techniques: Amnioscopy, blood analysis, estrogen determinations in urine, external and internal cardiotokography and internal pressure determinations, gas analyses of umbilical blood. amniotic fluid analyses (phospholipids), ultrasound (B-apparatus) and HCS determinations. All clinics dealing with risk pregnancies should have these techniques available. Total perinatal mortality decreased to below 2% after introduction of cardiotokography. During the last year it decreased to 0.89%. Premature mortality shows the same decrease and is 50% of total mortality. The frequency of premature deliveries remained unchanged at 6.2%. Both improved intensive monitoring and neonatal reanimation and intensive care contributed to the reduction of perinatal mortality. Continuous heart rate recordings make it possible to uncover hypoxic and acidotic states in time and this is of particular value for the premature infant. The incidence of acidosis (pH less than 7.10) was 2.03% before monitoring was introduced and fell to 0.45% this year when intensive monitoring became the rule. No pH lower than 7.0 was found this year. It is thus not sufficient to monitor only cases at risk, since in about 50% of infants born with acidosis no alarming symptoms were found that would have indicated the need for intensive monitoring. CONCLUSION: Infant mortality should be reduced to less than 1% if the diagnostic tools available are applied. Below this nonviable infants limit further improvement. Perinatal hypoxia and acidosis (below pH 7.10) should also be lower than 0.5% but at least lower than 1%.

Acidosis↗
PubMed1976

[Excrement of the meconium and the intra-partum cardiotocogram].

Of 2233 parturients, 170 cases with evidence of meconium during delivery were examined. We tried to establish the correlation between the appearance of meconium and the occurance of contraction induced FHR-decelerations in continuous intra partum monitoring. In addition we purposed to determine, whether the appearing of meconium during labor can be used as a clinical prognosite sign for fetal outcome. Our data support the assumption that the passage of meconium during any stage of labor only represents a sign of potential fetal distress showing the intra partum records pathology FHF-patterns. The appearance of meconium alone -- diagnosed at the time of the entrance of parturients or in an advanced stage of labor -- demands supplemented diagnostic methods for a better interpreting of an actual or compensated fetal distress.

Apgar Score↗
PubMed1975

[Severe incontinence of urine II degrees and its surgical treatment. (Indications for typical operations in cases of incontinence of urine and their results) (author's transl)].

Various surgical methods for the treatment of severe incontinence of urine II degrees can be used. The aim of the present study was to apply primarily in 255 cases of severe stress incontinence. II degrees the specific surgical procedure for treatment: 123 cases of cysto-rectocele repair including vaginal hysterectomy, 71 cases of puborectalis repair, 43 cases of urethrovesicosuspension operation with or without abdominal/vaginal supplementary procedures, till 1970 12 cases of combined operations and finally beginning in 1973 6 cases of dura-sling operation. Indications and principles of surgical intervention are described according to clinical intern procedures. Puborectalis repair (Franz operation) and pubococzygeus repair (Ingelman-Sundberg operation) in cases of missing prolapse combined with severe incontinence gave rather good results. The basis for optimum results after operative treatment of patients with stress incontinence is a detailed pre-operative diagnosis; The most specific operative procedure from the beginning seems to us more recommendable than routine cysto- rectocele repair including a second more specified operation in cases of relapse.

Age Factors↗
PubMed1975

[Is a vaginal breech delivery still justified? (author's transl)].

In our series of vaginal breech deliveries the morbidity from hypoxia and acidosis showed no difference to the same type of morbidity in our series of breech deliveries by Caesarean section. The correct selection of cases, intensive monitoring during labour and the management of the second stage of labour as outlined in our paper are the most important perequisites which permit to plan and justify a vaginal breech delivery in present obstetric practice.

Acidosis↗
PubMed1975

[Hypoxia and acidosis--morbidity (author's transl)].

A critical study based on 3291 analyses of blood from the umbilical cord, and the consequences. In routine analyses acidosis (pH 7.10) was found in 2.03%. This percentage is considered too high. Protracted labor carries special risks. Conventional and intensive care supervision, when risks are foreseen, are often ineffective, since 48.8% of acidoses were recognized only post partum. More general intensive observation with internal cardiotocography and microinvestigations of blood appears to be the only way in which the frequency of acidosis could be decisively reduced.

Acidosis, Respiratory↗