[Inhibition of reactive hyperemia by kallikrein inhibitors in canine oral tissue].
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Animals↗
Biomedical subjects
E Hiyama
Publications and source records attributed to E Hiyama.
Free radical damage to sarcoplasmic reticulum of masseter muscle by arachidonic acid and prostaglandin G2.
In vitro generation of free radicals by xanthine oxidase acting on hypoxanthine as a substrate induced a decreased calcium uptake velocity and reduced calcium-dependent ATPase activity of isolated sarcoplasmic reticulum (SR) vesicles from canine masseter muscle at pH 7.0. At pH 5.5 calcium uptake velocity was also reduced but ATPase activity was unaffected. Application of arachidonic acid or prostaglandin G2 induced the depression of both calcium uptake velocity and ATPase activity. The effect of arachidonic acid and prostaglandin G2 on ATPase activity depended on the pH. At pH 7.0, ATPase activity was decreased, but at pH 5.5 it was unchanged. These effects were reversed by superoxide dismutase (SOD) at pH 7.0, and by SOD plus mannitol at pH 5.5. Prostaglandin H2, prostaglandin E2 and 11,14,17-eicosatrienoic acid had no effect on calcium uptake velocity and ATPase activity at both pH 7.0 and pH 5.5. These results suggest that damage to the masseter muscle is caused by a free radical superoxide anion generated as a result of increased prostaglandins synthesis, and by the production of more lethal hydroxyl radical switched from the production of superoxide anion at low pH.
Adenosine Triphosphatases↗
Effect of peptide extracted from skeletal muscles of the fur seal on periodontal diseases.
It has been confirmed previously that a physiologically active peptide extracted from the skeletal muscles of fur seals exhibits excellent dermal absorption and has peripheral vasodilating properties. These properties were postulated to be beneficial to inflamed periodontal tissues. To test this, a dentifrice containing the peptide was prepared and utilized in a double-blind test. Significant improvement in the gingiva conditions was observed when a 0.1% or more concentration of peptide was used. As time progressed, distinct clinical improvement in redness, swelling, drainage, bleeding and pocket depth was noted. No significant side effects were noted although there were seen increased salivation and gingival blanching. It is believed, as a result of this and other studies, that the clinical efficacy of this peptide is due to its vasodilating action on the venules, thus reducing the internal pressure in the vascular beds.
Adolescent↗
Immunohistochemical detection of telomerase (hTERT) protein in human cancer tissues and a subset of cells in normal tissues.
We examined human telomerase reverse transcriptase (hTERT) protein distribution by immunohistochemistry in cultured cells and tissue sections. Cells with telomerase activity had nuclear positive signals whereas cells without telomerase activity did not. In most normal epithelial tissues, hTERT expression was prominent in the early proliferative descendent progenitors cells. In cancers with high telomerase activity, hTERT expression was detected in almost all neoplastic cells and correlated with telomerase activity levels, whereas cancers with low telomerase activity had fewer hTERT-positive cancer cells. In pediatric neuroblastomas with a favorable outcome, both the percentage of positive cells and the signal intensities of each hTERT-expressing cell decreased. These studies indicate that detection of telomerase at the cellular level is achievable and may have utility in cancer diagnostics.
Adult↗