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Biomedical subjects

E Hippe

Publications and source records attributed to E Hippe.

At least 91 records · Page 5Linked to original sources
PubMed1983

Plasma cobalamin and transcobalamin in patients with primary carcinoma of the liver. A study from Thailand.

Of 37 patients with histologically verified hepatocellular carcinoma (HCC) from Bangkok, Thailand, 34 had raised values of plasma cobalamin, and 1 presented with a markedly increased value of plasma transcobalamin I (TC I). One patient with clinical malignancy of the liver, not proven histologically to be HCC, had a raised plasma cobalamin value and a markedly increased value of TC I. From our own studies and from studies in the literature we find circumstantial evidence that TC I occasionally is produced by the malignant liver cells in HCC.

Adolescent↗
PubMed1983

Enzyme-linked immunosorbent assay (ELISA) for direct quantification of surface-bound platelet immunoglobulins.

Surface-bound platelet IgG and IgM were measured by an enzyme-linked immunosorbent assay (ELISA) using washed platelets and commercially available alkaline phosphatase anti-human immunoglobulins (Fc-specific). With this technique platelets from normal donors had small amounts of platelet-bound IgG ranging from 0.00 to 0.16 A405 (absorbance at 405 nm wavelength) (10(7) platelets)-1 (0 to 124 ng) and of platelet-bound IgM ranging from 0.00 to 0.05 A405 (10(7) platelets)-1. Eight out of 10 (80%) thrombocytopenic patients with idiopathic autoimmune thrombocytopenic purpura (IATP) had values of both IgG and IgM exceeding the normal range. In addition, one patient (8%) had platelet-bound IgM only. An inverse relationship was demonstrated in patients with IATP between the blood platelet count and the amount of both IgG and IgM. Increased values were also demonstrated in patients with SLE and patients with monoclonal hypergammaglobulinaemia. The direct ELISA is a useful and reproducible technique for platelet-bound IgG and IgM, which requires standard laboratory equipment only.

Adult↗
PubMed1982

Malignant centroblastic-histiocytic lymphoma of the skin.

A mixed B-cell type centroblastic lymphoma with extraordinarily many histiocytes in a 68-year-old man is reported. Multiple skin tumours were the only clinical manifestation during the first 5 months. The disease then progressed to the lymph nodes, spleen, and parenchymal organs, but the bone marrow remained unaffected until death, 11 months after the onset of signs. C3d receptors were the only surface markers of the centroblasts. The histiocytes were normal with respect to morphology, muramidase staining, and Fc and C3b receptors. This highly unusual spread from skin to lymphoid and parenchymal organs is discussed in the light of lymphoid cell kinetics.

Aged↗
PubMed1982

Cobalamin forms in plasma and tissue during treatment of vitamin B12 deficiency.

Thin-layer chromatography and bioautography were used to study the cobalamin pattern of plasma, erythrocytes, and hepatic tissue from patients with cobalamin deficiency on maintenance therapy with hydroxocobalamin (Vibeden), a cyanocobalamin depot preparation (Betolvex), or cyanocobalamin tablets (Behepan). The cobalamin pattern in plasma is dominated by the form in which it is administered. The results of assaying the erythrocytes and liver biopsies show that the cobalamins administered are converted to the coenzyme forms in vivo, irrespective of the type of cobalamin preparation.

Administration, Oral↗
PubMed1980

A case of cold haemoglobinuria with later sarcoidosis. Treatment with plasmapheresis and immunosuppressiva.

A case of severe but transient haemolytic disease occurring after a febrile episode is described. The thermal amplitude of the haemolysin was high during the acute phase, since the autoantibody fixed complement at 31 degrees C. After 4 months complement fixation could exclusively be demonstrated at 4 degrees C. The patient was treated in a room heated to 30-32 degrees C. The treatment consisted of prednisone and azathioprine and during the acute phase plasmapheresis was attempted in order to reduce the antibody concentration. However, the haemolysis decreased when the thermal amplitude of the antibody diminished. 1 year after termination of therapy, she developed sarcoidosis.

Adult↗
PubMed1980

Reversible dementia and neuropathy associated with folate deficiency 16 years after partial gastrectomy.

A 57-year-old woman developed dementia and peripheral neuropathy 16 years after a partial gastrectomy (Billroth II). Serum cobalamin was 198 pmol/l (reference interval 150-550), and the vitamin B12 absorption test (Schilling) showed decreased absorption (1.7% without and 2.2% with intrinsic factor). In spite of 20 months' therapy with vitamin B12, the neurological symptoms progressed. Folate deficiency was suggested by a very low erythrocyte folate and a slightly abnormal FIGLU test. There were no other signs of general malabsorption. A few months' treatment with folic acid significantly improved the massive neurological manifestations which were verified neurophysiologically as well as histologically. A common role of vitamin B12 and folate in the development of neuropathy is suggested.

Dementia↗
PubMed1980

The concentration of vitamin B12, transcobalamins and folate in blood in a Greenland Eskimo population sample. Increased cobalamin and transcobalamin II in plasma compared with a Danish reference group.

The concentrations of cobalamin and transcobalamin I and II in plasma and of folate in erythrocytes were determined in a Greenlander population sample. Compared with the Danish reference group, cobalamin and transcobalamin II were increased in the Eskimos, whereas there were no differences in transcobalamin I and erythrocyte folate.

Adult↗
PubMed1978

Increased concentration of transcobalamin I in a patient with metastatic carcinoma of the breast.

A patient with metastatic carcinoma of the breast and increased plasma cobalamin binding capacity (about 50 nmol/1) is described. The binding protein was identified as transcobalamin I (TCI) by DEAE cellulose ion-exchange chromatography, Sephadex G200 gel filtration and agar gel electrophoresis. Although the total plasma cobalamin concentration (about 20 nmol/1) was elevated, the patient complained of neurological symptoms in accordance with a functional vitamin B12 deficiency. Hence, an inactivation of the coenzyme is suggested by the demonstration of considerable amounts of 5'-deoxyadenosylcobalamin bound to the plasma TCI. Both urinary excretion of FIGLU and methylmalonic acid were within the reference ranges. Reported cases of increased cobalamin binding in patients with nonhaematological malignancy are reviewed. Further investigations to characterize the function of the cobalamin dependent metabolic pathways are necessary to determine the importance of the increased transcobalamin binding in these patients.

Blood Proteins↗
PubMed1978

Interrelationships between Vitamin B12 and folic acid in myelomatosis: cobalamin coenzyme and tetrahydrofolic acid function.

Cobalamin and folate metabolism was investigated in 43 patients with myelomatosis, in 8 control subjects of similar age and 22 younger controls. Plasma total cobalamin was lower in myeloma patients than in either of the control groups and methylcobalamin (Me-Cbl) was disproportionately reduced. Erythrocyte levels of total cobalamin were very similar in patients and elderly controls but were half the levels in younger controls. Erythrocyte levels of Me-Cbl were slightly higher in patients than in the dlderly controls. FIGLU excretion after L-histidine was elevated in 53% of the patients but values did not correlate with serum or erythrocyte folate or with plasma total cobalamin. FIGLU excretion decreased after DL-methionine or Me-Cbl only in patients whose FIGLU excretion was initially high. The results are discussed in the light of the 'methylfolate trap hypothesis' and suggest that some patients with myelomatosis have insufficient activity of methionine synthetase to meet the additional metabolic demand for one carbon compounds.

Adolescent↗
PubMed1977

Cobalamin binding proteins in stomach and serum from various animal species data for B12 binding capacities and molecular sizes of the binding proteins.

1. The unsaturated cobalamin-binding capacity of stomach mucosa and serum from 37 animals and the size of the binders have been measured. 2. The binding capacity in stomach mucosa was from 1 to 600 nmole kg-1 wet wt, the highest values occurring in pig, guinea pig, porpoise and earthworm. In serum it varied from 0.4 to 270 nmole 1(-1), the highest values occurring in duck, grass snake, toad and spiny dogfish. 3. The size in terms of Stokes radius of the cobalamin binders in stomach mucosa varied from 1 nm in hagfish (Mr approximately 14,000) to 7 nm in lamprey (Mr approximately 210,000). In serum it was from 1.4 nm (Mr approximately 17,000) to 7.4 nm (Mr approximately 240,000) both in frog.

Animals↗