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E Hess

Publications and source records attributed to E Hess.

At least 37 records · Page 2Linked to original sources
PubMed1978

Enzymatic sulfation of steroids. V. Partial purification and some properties of sulfotransferase III, the major glucocorticoid sulfotransferase of liver cytosols from male rats.

This manuscript describes purification of sulfotransferase III (STIII), the major hepatic glucocorticoid sulfontransferase of male rats, 77.8 +/- 16 fold from cytosol. This represents a probable 250--345 fold enrichment, compared with homogenates. Purified STIII has a molecular weight of 61 500 +/- 2500 from Sephadex G-100 chromatography. It is markedly activated by 5 mM divalent Ba, Ca, Co, Cr, Mg, Mn, and Ni salts; inhibited stronlgy by 5 mM divalent Zn and Cd; and unaffected by 8 mM ADP, ATP, and AMP. Comparison of the ability of purified STIII to sulfate equimolar cortisol, estradiol-17beta, testosterone, and dehydroepiandrosterone suggests that the enzyme may sulfate glucocorticoid preferentially. However, its cortisol sulfotransferase activity is inhibited by a variety of steroids. Of these, dehydroepiandrosterone, dexamethasone, and progesterone were tested extensively. They were found to be competitive inhibitors. STIII has a sharp pH optimum at pH 6.0 +/- 0.1. However, it is routinely assayed at pH 6.8, as explained in the text. It exhibits a sequential mechanism and Km values of 6.82 +/- 1.2 and 6.28 +/- 0.64 micron for cortisol and 3'-phosphoadenosine-5'-phosphosulfate, respectively. It also possesses essential sulfhydryl groups, as shown by p-hydroxymercuribenzoate inhibition studies.

Animals↗
PubMed1975

[Epidemiology of salmonellae and fertilizing of grasland with sewage sludge (author's transl)].

Our investigations prove that sludge contains Salmonellae in more than 90% of samples. The maximum number of organisms reached 10(7) per liter. One of our most important findings was the fact that neither aerobic stabilization nor anaerobic digestion significantly reduces the contamination with Salmonellae. Moreover we found that Salmonellae in sewage sludge spread on grass may survive up to 72 weeks. Fertilizing with unsanitized sludge may therefore lead to transmission from plant to animal. The increasing number of Salmonella carriers among our herds of cattle and their striking accumulation during the grazing period demonstrate that such transmission represents a growing danger. Sanitizing of sludge to be used as fertilizer is therefore urgent. We have investigated the sanitary effect of pasteurisation and of gamma irradiation on sewage sludge. After a proper pasteurisation in 5 plants (70 degrees C for 30 minutes) 98-100% of tested sludge samples contained less than 10 Enterobacteriaceae per gramm. The application of 300 krad resulted in a percentage of 97.2% of samples with less than 10 Enterobacteriaceae per gramm.

Animals↗