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Biomedical subjects

E Heilmann

Publications and source records attributed to E Heilmann.

At least 37 records · Page 2Linked to original sources
PubMed1983

Proliferation of haematopoietic colonies in serum-free cultures.

In eight patients with polycythaemia vera and 20 normals, mononuclear cells of the peripheral blood were cultured in methylcellulose with and without exogenously added erythropoietin. The cultures were set up with fetal calf serum and with a serum-free medium of unsaturated fatty acids, lecithin and cholesterol. In patients with polycythemia vera, we found a proliferation of haematopoietic colonies without erythropoietin in both culture systems which could not be seen in normals. The proliferation of endogenous colonies in serum-free media suggests that polycythaemia vera is a clonal disorder which can be modulated by serum factors and by erythropoietin.

Adult↗
PubMed1983

In vitro studies to prove the effect of sera of eosinophilia in colony formation.

Serum samples from four patients with reactive eosinophilia and two patients with eosinophilic leukaemia were compared with normal sera with respect to formation of eosinophil colonies after addition of the sera to mononuclear cells from peripheral blood of healthy subjects. Supernatants from ConA stimulated guinea-pig spleen cells and human lymphocytes were tested in a similar way. Grown colonies were placed on glass slides and after staining with luxol fast blue the percentage of eosinophils was counted. The serum samples of the patients with reactive eosinophilia produced the greatest number of eosinophil colonies while supernatants of spleen and lymphocytes produced the greatest number of eosinophilic granulocytes. Our findings suggest the existence of a factor stimulating eosinophil colonies in the tested serum fractions. Beyond that an indication is given for a substance in the supernatants of spleen and lymphocyte suspensions which stimulates more intensively the maturing into eosinophilic granulocytes than the formation of colonies.

Colony-Stimulating Factors↗
PubMed1983

[Effects of extracorporeal blood irradiation on the lymphocyte population in chronic lymphadenosis].

The surface markers of lymphocytes were detected in 18 patients with chronic lymphatic leukemia (CLL), with 9 of them being treated by means of extracorporeal blood irradiation (ECIB) and 9 of them being without any treatment. We found that B-lymphocytes were eliminated by ECIB to a higher degree than it can be recognized by the decrease of lymphocytes. As to their percentage B-cells with ME-receptors or EAC-receptors respectively were partially diminished to a higher degree than the total population of B-cells. Moreover, the pathological Ig-type could be shown to be affected predominantly. The changes of marker profiles and absolute values achieved by ECIB revealed an approach of values in treated patients to those of untreated patients. The investigations make it clear that the determination of lymphocyte subpopulation can be used not only for diagnosis and differential diagnosis of lymphatic system diseases, also for evaluating therapy effects.

Chronic Disease↗
PubMed1983

Morbidity of chronic myeloid leukemia.

In 129 patients with chronic myeloid leukemia recorded in our files between 1955 and 1978, the sex ratio showed a slight decrease of the previously higher proportion of males. The morbidity rates of the two sexes are approaching. A shift of the incidence peak in males to a younger age is a striking feature, in contrast to a shift to the opposite direction in females. The incidence peak of women shifted from 35 years in the first to 65 yr in the second period, for men from 55 to 35 yr. The mean age at diagnosis was higher in women than in men. A decrease of frequency was observed during the second period 1970-1978. Whether this is a real improvement and consistent trend has to be ascertained by further studies.

Adolescent↗
PubMed1983

Primary polycythaemia in childhood and adolescence.

Four adolescents with polycythaemia are described where symptoms typical of the disease had already existed in childhood. Three patients fulfilled the criteria of a polycythaemia vera. In one female patient the possibility of familial erythrocytosis is discussed. Polycythaemia vera mononuclear cells formed erythroid colonies in cultures in the absence of erythropoietin. The patients were phlebotomized to maintain haematocrit readings of 40 to 45%. No case has yet progressed to myeloid metaplasia or acute leukaemia.

Adolescent↗
PubMed1983

Studies in polycythemia after kidney transplantation.

Mononuclear cells from peripheral blood of six patients with polycythemia after kidney transplantation were studied in methyl cellulose cultures using fetal calf serum with and without adding erythropoietin. Parallel tests were run with "synthetic medium". The data obtained were compared to those of patients with polycythemia vera and normal subjects. The erythropoietin independent proliferation in synthetic medium typical for polycythemia vera could not be found in patients with renal grafts. However, in the preparations with fetal calf serum without erythropoietin, a formation of erythroid colonies could be detected suggesting an increased sensitivity or proliferation of erythropoietically determined stem cells in patients with transplanted kidneys.

Adult↗
PubMed1982

[Hereditary spherocytosis and polycythemia].

A case of hereditary spherocytosis is described in a casuistic report where the clinical picture of polycythaemia appeared 5 years after a successful splenectomy. The possibilities of a coincidence of diseases, a postsplenectomy reaction or a genuine myeloproliferative syndrome as the cause underlying a secondary disease are discussed.

Adult↗
PubMed1982

[In vitro studies of cells in histiocytosis X].

In a female patient with histiologically ensured histiocytosis X, cell cultures modified according to NEZELOF were performed in methylcellulosis and Eagle's medium. From the 5th to the 15th day of incubation the cells were daily observed natively through a normal microscope and taken for histochemical examinations and for checking their capacity of phagocytosis. Two cell types could be distinguished: spindle-shaped fibroblasts as well as round cells of changing form and size, the alpha-naphthylacetate esterase response of which proved to be positive. The round cells can partly be regarded as HK cells, whereas giant cells could be predominantly observed during the second week. A phagocytosis of iron chips and human erythrocytes loaded with antibodies could be revealed in both types. HK cells represented mature macrophages, from which giant cells developed under in vitro conditions. There is still not explanation as to the local and immunological factors characterizing the clinical picture of histiocytosis X.

Adult↗
PubMed1982

[Cultured lymphocytes in chronic alcoholics].

Lymphocytes were isolated from the peripheral blood of 80 chronic alcoholics without an evident liver damage and filled up with 4 different mitogens. The DNA synthesis was investigated by means of the 3H thymidine incorporation rate. They were referred to a group of 15 healthy test persons. An increased proliferation rate of B-lymphocytes could be demonstrated, whereas there was no evidence for a change in the stimulatibility of T-lymphocytes. It was only the direct impact of ethyl alcohol on lymphocytes that produced a significant decrease in the ability of T-cell reactivity, whereas a further increase of stimulatibility could be observed in B-cells. Our findings allow the conclusion to be drawn that an immediate toxic impact of ethanol on lymphocytes can be identified in chronic alcoholics without manifest liver damage.

Adult↗
PubMed1982

[Hypereosinophilic syndrome. Case report of the differential diagnosis of eosinophilic leukemia].

We report on a 48 years old patient with chronic eosinophilic leukemia. The diagnosis is based on changes of the blood picture with leukocytosis, deviation to the left to promyelocytes and sometimes normoblasts in the differential smear in with eosinophilia of up to 90%. A hepatosplenomegaly with an extensive extramedullary blood formation could be observed. Endocarditis parietalis fibroplastica was found on autopsy. The problem whether the organ changes observed are due to the eosinophilia existing for more than two years is discussed.

Cell Transformation, Neoplastic↗
PubMed1981

[Erythropoietin assay. Comparison of a biological test system with an haemagglutination inhibition test (author's transl)].

The results from a biological test for erythropoietin (using the rate of iron absorption in polycythemic mice) and a commercially-available immunological test (haemagglutination-inhibition test) were compared. Of 19 batches of the immunological test which were investigated, 7 batches were completely inactive and a further 3 batches reacted only with the test serum supplied with the test. There was a poor correlation between the results from the biological and the immunological measurements, both on patients with high and those with low serum erythropoietin levels. The difficulty of the immunological erythropoietin test is that pure erythropoietin is not sufficiently available. The immunological test investigated here does not use pure erythropoietin. Aside from this, pathophysiological considerations would lead one to expect basic differences between the results from immunological and biological tests.

Adrenal Gland Neoplasms↗
PubMed1981

[Stimulation of hematopoietic stem cells with serum specimens of patients with polycythemia vera].

Mononuclear cells from the peripheral blood of normals and patients with terminal renal insufficiency on hemodialysis were set up in methylcellulose cultures with and without addition of sera of patients with polycythemia vera (pv). In renal patients we found more intensive stimulation of hematopoietic progenitor cells after addition of pv-sera than in normals. The findings suggest a hematopoietic factor in sera of pv which is not identical with erythropoietin.

Cells, Cultured↗
PubMed1981

[Long-term cultures in polycythaemia vera as proof for dependence on erythropoietin (author's transl)].

In eight patients suffering from polycythaemia vera, the proliferation of erythroid colonies in methylcellulose with and without exogenous erythropoietin was studied. After three and five weeks, respectively, no colonies grew. Due to addition of erythropoietin more erythroid colonies proliferated which could be cultivated over nine weeks. The experiments suggest that the erythropoiesis in polycythaemia vera depends on erythropoietin.

Adult↗