[The comeback of surgical treatment for Parkinson disease].
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Basal Ganglia↗
Biomedical subjects
E Heikkinen
Publications and source records attributed to E Heikkinen.
Increase of collagen synthesis and deposition in the arachnoid and the dura following subarachnoid hemorrhage in the rat.
Arachnoidal fibrosis following subarachnoid hemorrhage (SAH) has been suggested to play a pathogenic role in the development of late post-hemorrhagic hydrocephalus in humans. The purpose of this study was to investigate the rate of collagen synthesis in the arachnoid and the dura in the rat under normal conditions and to study the time schedule and the localization of the increased collagen synthesis following an experimental SAH. We found that the activity of prolyl 4-hydroxylase, a key enzyme in collagen synthesis, was 3-fold higher in the dura than that in the arachnoid and was similar to the activity in the skin. We then induced SAH in rats by injecting autologous arterial blood into cisterna magna. After SAH, we observed an increase in prolyl 4-hydroxylase activity of the arachnoid and the dura at 1 week. At this time point the enzyme activity in both tissues was 1.7-1.8-fold compared to that in the controls and after this time point the activities declined but remained slightly elevated at least till week 4. The rate of collagen synthesis was measured in vitro by labeling the tissues with [(3)H]proline. The rate increased to be 1.7-fold at 1 to 2 weeks after the SAH in both of the tissues. Immunohistochemically we observed a deposition of type I collagen in the meninges at 3 weeks after the SAH. SAH is followed by a transient increase in the rate of collagen synthesis in the arachnoid and, surprisingly, also the dura. Increased synthesis also resulted in an accumulation of type I collagen in the meningeal tissue, suggesting that the meninges are a potential site for fibrosis. The time schedule of these biochemical and histological events suggest that meningeal fibrosis may be involved in the pathogenesis of late post-hemorrhagic hydrocephalus.
Animals↗
An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications.
Transposons are mobile genetic elements and have been utilized as essential tools in genetics over the years. Though highly useful, many of the current transposon-based applications suffer from various limitations, the most notable of which are: (i) transposition is performed in vivo, typically species specifically, and as a multistep process; (ii) accuracy and/or efficiency of the in vivo or in vitro transposition reaction is not optimal; (iii) a limited set of target sites is used. We describe here a genetic analysis methodology that is based on bacteriophage Mu DNA transposition and circumvents such limitations. The Mu transposon tool is composed of only a few components and utilizes a highly efficient and accurate in vitro DNA transposition reaction with a low stringency of target preference. The utility of the Mu system in functional genetic analysis is demonstrated using restriction analysis and genetic footprinting strategies. The Mu methodology is readily applicable in a variety of current and emerging transposon-based techniques and is expected to generate novel approaches to functional analysis of genes, genomes and proteins.
Base Sequence↗
A path analysis model of self-rated health among older people.
The aim of this study was to examine the structure of self-rated health among 75-year-old men and women. The study was part of the Evergreen project, comprising all the 75-year-old residents (N = 382) of Jyväskylä, in central Finland, in 1989. The data were collected by interviews, questionnaires and laboratory examinations, focusing on different domains of health and functional capacity. Of the target group, 91.6% (119 men and 231 women) participated in the interview, and 77.2% (104 men and 191 women) took part in the clinical and laboratory examinations. Path analysis models (LISREL 8) were used to examine the structure of self-rated health. About half the participants self-rated their health as good or unusually good. In both genders, a smaller number of difficulties in performing the physical activities of daily living (PADL), fewer chronic diseases, and better maximal working capacity were associated with better self-rated health. In addition, among the women a smaller number of depressive symptoms, and among the men better cognitive capacity had a positive effect on self-rated health. Physical activity and muscle strength had a positive indirect effect on self-rated health among both men and women. The explanatory power of the path analysis model for self-rated health was 44% for the men, and 42% for the women. The ability to perform the physical activities of daily living independently is an important associate of good self-rated health among older people. The path analysis models suggest that the factors underlying the physical activities of daily living also directly modify self-ratings of health among the elderly. The models of self-rated health for the men and the women were not exactly alike.
Aged↗
Absence of p53 mutations in benign and pre-malignant male genital lesions with over-expressed p53 protein.
Mutations of the tumor-suppressor gene p53 are common in epithelial tumors. Clonal mutations of p53 have been found in cervical and vulvar carcinomas negative for human papillomavirus (HPV), though at least in cervical cancer HPV infection and p53 mutations are not mutually exclusive. We have previously shown that about 40% of male genital warts and bowenoid papulosis lesions exhibit immunohistochemically detectable aberrant p53 protein, irrespective of the presence of HPV DNA. We studied p53 mutations in exons 4-8 with SSCP and sequencing in 13 male patients with 1 to 3 therapy-resistant genital warts or intra-epithelial neoplasias each and in 4 patients with penile squamous cell carcinoma. Thus, 13 genital warts, 6 bowenoid papulosis, 1 Queyrat's erythroplasia and 1 carcinoma in situ were studied. p53 protein was detected immunohistochemically, and HPV status was analyzed with DNA in situ hybridization and amplification of HPV-specific DNA. There was no correlation between p53 protein expression and HPV status. No mutations in exons 5-8 of the p53 gene were found in any of the lesions, and furthermore, no exon 4 mutations were found in lesions positive in p53 immunohistochemistry. In conclusion, overexpression of p53 does not indicate a p53 mutation in male genital warts, pre-malignant lesions or malignant squamous cell carcinomas. Our study thus suggests that p53 mutations are not important, or at least not early, events in male genital carcinogenesis.
Biopsy↗
The Y chromosomes of Drosophila lummei and D. novamexicana differ in fertility factors.
Many first-generation interspecific hybrids between the five species of the Drosophila virilis subgroup are fertile. Fertile hybrids can be utilized in the analysis of sterile hybrids, by creating new combinations of genetic elements to identify cross-incompatible chromosomes or chromosome segments. Here, crosses starting from D. lummei, D. virilis, D. novamexicana and D. americana were produced to analyse the 96.4% male sterility in the progeny of D. virilis females mated with D. novamexicana males (sterility based on sperm motility). In autosomally identical heterozygous combination, the substitution of a large segment of the D. virilis X chromosome with a double or a triple inversion from the sister taxa decreased male sterility from 96.4% (Xvirilis) to 11% (Xvirilis/novamexicana), 19% (Xvirilis/americana) and 34% (Xvirilis/lummei). This result shows that the Xvirilis interaction with D. novamexicana elements (Y and/or autosomes) is a major component in the F1 sterility observed. When males with a substituted Ylummei in otherwise pure D. novamexicana were crossed with D. virilis females, all the F1 males were fertile. We constructed combinations of intact Xvirilis, recombinant Xvirilis/novamexicana, Ynovamexicana and Ylummei chromosomes in homozygous D. virilis and heterozygous D. virilis/D. novamexicana autosomal backgrounds. In such combinations, the Y chromosome interacted strongly not only with the nonconspecific X chromosome, but with several autosomes. Autosomal hybrid sterility contributions tend to be recessive and not present in the F1. In this species group, the role of the Y chromosome is central in contributing to the phenomenon called Haldane's rule of sterility, but the Y chromosomal incompatibility factor of D. novamexicana is the most divergent one.
Animals↗
'A good person does not feel envy': envy in a nursing community.
The definition of envy is commonly based on psychoanalytical views of organizational culture. The purpose of this paper is to describe envy in a nursing community. The population study consisted of random sample of 120 subjects drawn from among the employees of one central hospital in Finland. The study material consisted of data collected by questionnaires (response rate 65%). The data were processed by various statistical methods. Open-ended questions were analysed by inductive content analysis. The results of this study indicated that the employee's view of his/her official position in the nursing community, his/her relationships with his/her fellow workers and the management as well as relationships with other nursing communities are all related to envy. The employee's view of his/her official position intensified his/her feelings of envy, if he/she had other negative feelings, anxiety, dissatisfaction with him/herself, and negative feelings towards fellow workers. The major object of envy was fellow workers' salaries. Envy towards other nursing communities was generated by alleged differences in the amount of labour, or by the charge nurse's greater interest in other sections. Employees coped with envy by hiding these feelings and being modest. Women coped with envy by being silent, while men denied the value of the object of envy.
Adaptation, Psychological↗
Associations of sensory-motor functions with poor mobility in 75- and 80-year-old people.
This study investigated the associations of sensory-motor functions with mobility in elderly people. All 75- and 80-year-old residents of the city of Jyväskylä, Finland, were invited to take part in the study. A total of 617 (93%) persons were interviewed, and 500 (75%) took part in laboratory examinations. Self-reported mobility was recorded during the interview. Basic mobility functions (maximal walking speed and stair-mounting ability) and sensory-motor functions (maximal isometric muscle strength, standing balance, reaction time and visual acuity) were measured in the laboratory. Multivariate analyses showed that poor sensory-motor functions were significantly associated with poor performance in basic mobility functions and that poor performance in basic mobility functions was associated with self-reported disability in mobility. The associations discovered were consistent with models of the disablement process. Muscle strength, balance, reaction time and vision all have individual significance for mobility, underlining the need for multifactorial approaches in prevention and rehabilitation.
Activities of Daily Living↗
Is self-rated health comparable across cultures and genders?
Self-rated health is a frequently used health indicator, but there is little data on its comparability across cultures. We employed samples from Tampere, Finland, and Florence, Italy, of the European Longitudinal Study on Aging to examine the cultural and gender differences in self-rated health. Personal interview data was used and vital status ascertained after 7 years. After adjusting for several health-related variables, we found no substantial difference in self-rated health between genders, although women in Florence were three times and men in Florence four times more likely to report good self-rated health than men in Tampere. The correlational structure of self-rated health was similar in both areas. The significant graded association between self-rated health and mortality in both areas was mostly explained by other health indicators included in a multivariate model. Results suggest that self-rated health is a useful summary of physical health, but it may predict mortality better in men than in women and be sensitive to cultural environment. Therefore, direct gender and cultural comparisons of self-rated health should be made with caution.
Aged↗
Constant hierarchic patterns of physical functioning across seven populations in five countries.
This research was aimed at identifying critical steps in the decline in physical function that often parallels aging. Six basic and nine instrumental activities of daily living (ADLs) were classified into four domains of disability characterized by specific underlying physical impairment. The hierarchical order of this classification was verified in two random samples representative of the older home-dwelling population. The concordance level of disability and results of performance-based measures of physical function were also tested. Finally, the cross-cultural reliability of the model was verified in seven population-based samples of older persons living in five European countries. In older persons the disabling process follows a general pattern of progression based on a typical sequence of impairments.
Activities of Daily Living↗
Maintaining movement ability in old age: challenges for gerontechnology.
The degree of behavioral slowing that occurs with aging is highly task specific. Compensation for age related slowing, when necessary, requires task redesign and ergonomic interventions. Walking, performing various physical chores required in carrying out ADLs and IADLs, and avoiding falls are challenges for significant numbers of old people. Interventions based on prevention and/or compensation were presented. Approaches to the maintenance of strength required to carry out such activities emphasized resistive training programs. Improvements in visibility of the physical environment were stressed as one means of reducing falls and accidents.
Activities of Daily Living↗
A comparative study of factors related to carrying out physical activities of daily living (PADL) among 75-year-old-men and women in two Nordic localities.
The aim of this cross-sectional and cross-national study was to describe and compare the ability to carry out physical activities of daily living (PADL) and examine factors that might explain variation in this ability in two Nordic populations. Seven hundred and six men and women aged 75 from two populations (Glostrup, Denmark, and Jyväskylä, Finland) were interviewed and given a laboratory examination in 1989-90. The ability to carry out the PADL activities was studied by interview. Tests were given to determine depressive symptoms, cognitive capacity, and selected physical and sensory performance domains. Four different regression models (men and women in Jyväskylä and Glostrup) were used to analyze a number of variables describing physical and psychological health and performance related to the PADL. Knee extension strength and stair mounting height in three models (men and women in Jyväskylä and women in Glostrup), and walking speed in one model (men in Glostrup) emerged as explanatory factors on the basis of the physical performance tests done. Sight, except in the women in Glostrup, hearing in the men in Jyväskylä, and balance in the women in Glostrup also explained PADL functioning. In addition, symptoms of depression in the men in Glostrup, and symptoms of illness, except in the men in Jyväskylä, and cognitive capacity in the men in Jyväskylä emerged as explanatory factors in the regression models. There were, however, no major differences in the determinants of PADL functioning in the two Nordic populations of elderly people. Physical, psychological and sensory tests provide useful information, complementary to self-reports regarding declining PADL functional capacity.
Activities of Daily Living↗
Postural balance and self-reported functional ability in 75-year-old men and women: a cross-national comparative study.
OBJECTIVE: To study postural balance in relation to self-reported functional ability (mobility and ADL) and general physical activity in elderly men and women living in three different Nordic environments. PARTICIPANTS: A random sample of 448 men and 556 women from among the 75-year-old residents in Glostrup, Denmark, and Göteborg, Sweden, and all the residents of relevant age (127 men and 261 women) in Jyväskylä, Finland. MEASUREMENTS: Assessment of postural balance with eyes open and closed using a piezoelectric force platform. A structured interview on self-reported functional ability and physical activity. An in-laboratory medical examination. RESULTS: In spite of some differences in balance between the groups studied (better results in women compared with men and, to some extent, better results in the participants from Denmark and Finland than in those from Sweden), the performance in the balance tests was similarly associated with functional ability within all groups. The subjects reporting no need of help in performing the ADL and mobility functions performed significantly better in the balance tests. These differences were seen more clearly in the control of anteroposterior movement of center of forces than in the mediolateral direction. The performance in the balance tests was also significantly better among the subjects reporting a higher level of general physical activity than in their less active counterparts. Physical activity and than in their less active counterparts. Physical activity and certain long standing illnesses modified significantly the relationship between postural balance and ADL-performance. When these factors were analyzed simultaneously, the role of balance as a predictor of ADL-performance largely disappeared. CONCLUSIONS: The results suggest that good balance is one of the prerequisites of performance without difficulty in mobility and ADL functions. Physical exercise may help to maintain balancing abilities in old age; good balance, in turn, may also enable a physically active way of life. The associations of balance with functional ability and physical activity were independent of sex and locality. The results also support the validity of static stabilometry as a tool for evaluating threats to functional limitations in older subjects.
Activities of Daily Living↗
Physical activity and the changes in maximal isometric strength in men and women from the age of 75 to 80 years.
OBJECTIVE: To research the natural changes in maximal isometric strength, over a period of 5 years, in men and women aged 75 at baseline, and to study the effect of everyday physical activity on strength alterations. DESIGN: A 5-year longitudinal study. SETTING: Exercise laboratory. PARTICIPANTS: The target group in 1989 was the total 75-year-old population of Jyväskylä. One hundred one men (81%) and 186 women (75%) participated in baseline strength tests, and after 5 years, 55 men and 111 women (70% and 72% of the survivors) took part in the follow-up measurements. METHODS: Maximal isometric hand grip, arm flexion, knee extension, trunk flexion, and trunk extension forces were measured using dynamometers. Self-rated physical activity was recorded using a scale by Grimby (1986). Strength changes were compared between groups based on the amount of everyday physical activity: (1) remained active (AA, 24 men, 24 women); (2) remained sedentary (SS, 11 men, 43 women); (3) decreased activity (AS, 11 women); and (4) increased activity (SA, 32 women). AS and SA could be formed for women only because of the small number of men. All analyses were stratified by gender. MAIN RESULTS: The average percentage change in strength over 5 years among survivors varied from a 4% increase in knee extension strength observed in men and women to a 16% decrease in grip strength in women. The grip strength decrease was greater in women than men. The AA men maintained their trunk extension strength at a higher level than the SS men. Time by group interactions in men were not significant. In women, the rate of decline in AS was 32% in grip and 27% in elbow flexion strength, which was greater than in the other activity groups. The AA women retained their knee extension strength at a higher level than the other groups. Those who died before follow-up tests exhibited poorer strength test results at baseline. Physical activity decreased over follow-up. CONCLUSIONS: Strength alterations with age differed between muscle groups. Undertaking everyday physical activities such as household work, walking, and gardening, which are also the most common physically demanding activities of older people, may play an important role in maintaining strength at an adequate level for independent living.
Aged↗
Calcaneal bone mineral density predicts fracture occurrence: a five-year follow-up study in elderly people.
A 5-year follow-up study investigated calcaneal bone mineral density (BMD) and changes in BMD in relation to fracture occurrence. The subjects comprised two cohorts born in 1914 and 1910 living in the city of Jyväskylä in central Finland. One hundred and three men (82%) and 188 women (73%), aged 75, and 57 men (74%) and 136 women (65%), aged 80, of the eligible population participated in the baseline bone measurements. The follow-up bone measurements were obtained for 59 men (68%) and 119 women (66%), aged 80 years, and for 21 men (53%) and 61 women (48%), aged 85 years. During the follow-up period, 8 men and 36 women from the younger and 11 men and 24 women from the older cohort sustained at least one fracture. When the baseline levels of BMD were related to fracture occurrence, the results clearly showed that with increased BMD values the probability of fracture decreased. Where men and women had similar BMD values, they also had a similar fracture probability. Except for one woman in the older cohort, none of those who had initial BMD values more than 1 standard deviation above the mean for their age developed a fracture during the follow-up period. The mean annual decrease in BMD was greater in the women (2.5-2.7%) than in the men (0.8-1.0%). The BMD change tended to associate with fracture occurrence only in the 75-year-old women (p = 0.075). The results suggest that calcaneus BMD can be used as a predictor of fracture occurrence in 75- to 80-year-old men and women. However, associating fractures with the change in BMD was difficult due to the limited number of survivors and initial differences in BMD values.
Aged↗
Socio-economic status, health and life-style in two elderly cohorts in Jyväskylä.
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Activities of Daily Living↗
Functional capacity and health of elderly people--the Evergreen project. Background, design and methods of the project.
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Activities of Daily Living↗