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Biomedical subjects

E Hedfors

Publications and source records attributed to E Hedfors.

At least 55 records · Page 3Linked to original sources
PubMed1983

Phenotypic characterization of cells within subcutaneous rheumatoid nodules.

An immunohistochemical double-staining technique was applied to frozen sections of biopsies from subcutaneous rheumatoid nodules. Material within the central necrosis reacted with anti-immunoglobulin and anti-HLA-DR antibodies. Cells within the palisading layer were "macrophage-like," as indicated by staining for anti-HLA-DR and OKM1, and showed a high proliferative activity as seen by OKT9 reactivity. The granulation zone contained many (alpha Leu-1+) T cells in close contact with HLA-DR-expressing non-T cells.

Antibodies, Monoclonal↗
PubMed1983

Physiological variation of blood lymphocyte reactivity: T-cell subsets, immunoglobulin production, and mixed-lymphocyte reactivity.

Highly purified blood lymphocytes from healthy individuals were obtained from samples collected before and after a standardized bicycle ergometer test. In accordance with previous findings physical work resulted in a marked increase of circulating lymphocytes, with a proportionate decrease of T lymphocytes and increase of non-T lymphocytes including B cells. The decrease of T lymphocytes was accounted for by a reduction of cells reactive with the monoclonal OKT4 serum (helper/inducer), whereas the proportion of OKT8-positive cells (suppressor/cytotoxic) remained unchanged. The lymphocyte production of IgG, IgM, and IgA after 7 days culture with pokeweed mitogen and the lymphocyte DNA synthesis after activation by allogeneic cells was reduced during work. It is concluded that nonspecific stress changes the composition of T-lymphocyte subsets with depression of T-cell stimulation and T-cell-dependent immunoglobulin production.

Antibodies, Monoclonal↗
PubMed1983

HLA-B8/DR3 in sarcoidosis. Correlation to acute onset disease with arthritis.

HLA-A, B, C and DR typing was performed in 19 patients with sarcoidosis of acute onset. Besides bilateral hilar lymph node enlargement (BHL), ankle joint involvement was found in all and erythema nodosum in seven. HLA-B8 was present in 13 and HLA-DR3 in 17 of the 19 patients. The joint manifestations and the erythema nodosum subsided in all within three months. The BHL regressed completely in seventeen patients during a three year observation period. In two patients with an observation period of one year and less than six months, respectively, BHL were still visible. The strong association between the acute, prognostically beneficial manifestation of sarcoidosis and HLA-DR3 might partly be related to the presence of circulating immune complexes in acute sarcoidosis as well as to an immunogenetically determined handling of a postulated etiological antigen.

Adult↗
PubMed1980

[Sarcoidosis].

Explore the source record for details and available documents.

Humans↗
PubMed1977

Anticoagulant treatment in sarcoidosis.

The treatment of sarcoidosis has so far been disappointing. This paper presents a patient in whom persistent disappearance of the pulmonary infiltrations was observed during anticoagulant treatment for venous thrombosis. A possible mechanism for a beneficial effect of anticoagulant treatment in sarcoidosis is discussed.

Adult↗
PubMed1977

The effect of adrenaline, insulin and hydrocortisone on human peripheral blood lymphocytes studied by cell surface markers.

Changes in numbers of peripheral blood lymphocytes from healthy individuals were calculated from samples collected before and after parenteral administration of adrenaline, insulin and hydrocortisone, respectively. A marked increase in circulating lymphocytes was noted in response to adrenaline and insulin. However, subpopulation analysis showed a decrease in the proportion of T-lymphocytes, estimated as cells forming rosettes with sheep red blood cells after incubation in the cold and a corresponding increase in proportion of lymphocytes having receptors for C3 (non-T lymphocytes). In contrast, lymphocyte numbers were unaffected by hydrocortisone. The results indicate that a decreased proportion of circulating T-lymphocytes and an increase of non-T-lymphocytes may be the result of adaptive changes in response to various forms of stress and hence is to be expected in several clinical conditions.

Adult↗
PubMed1976

Characterization of peripheral blood lymphocytes in sarcoidosis.

In the basal state, patients with sarcoidosis have reduced numbers of circulating T lymphocytes and a normal number of circulating B lymphocytes. The lymphocytes show impaired DNA synthesis in response to several mitogens and antigens and impaired K-cell cytotoxicity. Circulating atypical cells of lymphocytic origin are present belonging to both T- and B-cell lineages. After work (with mobilization of lymphocytes) the T-cell deficiency is further accentuated compared with normals. Moreover, after work a deficiency in the number of circulating B cells is also encountered. The atypical cells do not increase. Compared with normals the impaired K-cell cytotoxicity and lower levels of DNA synthesis also persist. It is suggested that the deviations found in sarcoidosis are consequences of a disease process affecting the lymphoid tissues and successively replacing the normal structures. The presence in vivo of DNA-synthesizing circulating lymphocytes may indicate a persistent antigenic stimulation.

Antigen-Antibody Reactions↗
PubMed1976

Lymphocyte mobilization in response to physical work in sarcoidosis. Influence on lymphocyte subpopulations, DNA synthesis and cytotoxicity.

Peripheral blood lymphocytes of nine patients with pulmonary sarcoidosis were collected before and after a standardized bicycle ergometer test. The lymphocytes were characterized by various cell surface markers as T or B lymphocytes, and functionally by mitogen- or antigen-induced DNA synthesis and by antibody dependent cell-mediated cytotoxicity. Physical work resulted in an increase of circulating lymphocytes. Proportionately more B lymphocytes were mobilized. The DNA synthesis of lymphocytes in response to mitogens and antigen was reduced, whereas the cytotoxicity was augmented. Compared with normals the mobilization of lymphocytes was less, and a deficiency of both T and B lymphocytes was revealed. The functional impairment of lymphocytes in the patient group persisted.

Adult↗
PubMed1976

Variations of blood lymphocytes during work studied by cell surface markers, DNA synthesis and cytotoxicity.

Highly purified peripheral blood lymphocytes from healthy individuals were obtained from samples collected before and after a standardized bicycle ergometer test. The physical activity resulted in a marked increase of circulating lymphocytes. The proportion of T lymphocytes estimated as cells forming rosettes with sheep red blood cells after incubation in the cold decreased, whereas a corresponding increase of cells with receptors for C3, IgG-Fc or surface immunoglobulin was noted. Moreover, after work an increase of cells simultaneously reacting with cell surface markers usually designed as T- or B-cell markers occurred. The reactivity of lymphocytes collected after work in response to Con A, PHA, PWM and PPD was impaired, whereas the slight response to LPS was unchanged. The K-cell cytotoxicity of lymphocytes collected after work increased. The data indicate that physical activity leads to the mobilization of lymphocytes from as yet undetermined sites and with changed composition and reactivity.

Adult↗
PubMed1975

Activation of human peripheral blood lymphocytes by concanavalin A dependence of monocytes.

The activation of human peripheral blood lymphocytes or isolated T lymphocytes by concanavalin A (Con A) is hightly potentiated by the presence of autologous, mitomycin C-treated monocytes. The optimal lymphocyte: monocyte ratio within a broad dose range is 1:1 when the incorporation of [14C]thymidine is expressed as total incorporation per culture tube and 1:10 when expressed per lymphocyte. A five-to-ten-fold increase of total DNA synthesis is noted in the presence of 10-90% monocytes. The data may help to explain the wide variations in Con A responsiveness of human peripheral lymphocytes which may be partly related to differences in purification which give rise to cell preparations containing varying amounts of monocytes.

Cell Fractionation↗
PubMed1975

Immunological aspects of sarcoidosis.

Circulating immune complexes were found in sera from patients with acute sarcoidosis during the initial phase of the disease. The symptoms of acute sarcoidosis may be explained by the presence of such complexes. The total number of peripheral T cells was lower in most patients with sarcoidosis than in healthy controls. The difference was most marked in cases with long duration of the disease. The composition of peripheral T cells was altered irrespective of the total number of such cells, and a lack of cells responding to PPD and con A was shown. Tis may be related to the impairment of delayed hypersensitivity reactions which may be present in all stages of the disease. The atypical mononuclear cells which have been found in blood from patients with sarcoidosis are lymphocytic cells. Some have B cell properties whereas some seem to have T cell origin. The presence of such cells may indicate a persistent activation of the immune system. Immunological reactivity determined by genes closely linked to the major histocompatibility complex does not seem to be responsible for susceptibility to sarcoidosis.

Acute Disease↗