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Biomedical subjects

E Hansson

Publications and source records attributed to E Hansson.

At least 145 records · Page 8Linked to original sources
PubMed1983

Incorporation of 3H-valine into soluble protein of cultivated astroglial cells after morphine treatment.

Uptake and incorporation of 3H-valine into soluble protein of cultivated astroglial cells from newborn rat cerebral hemispheres increased after incubating the cells for 4 hr in 10(-6) or 10(-5) M morphine. The effects, inhibited by high naloxone concentrations, were not mediated by cyclic adenosine monophosphate (cAMP)-activated binding sites for morphine or D-alanine2-methionine-enkephalin-amide (D-ala2-met-enk-amide). The cultures consisted of 60-70% astrogliallike cells. By changing the kinetics of labeling it was shown that morphine affects protein synthesis of the cells in a concentration- and time-dependent way. Even in the incubation medium increased amounts of labeled proteins were found in a time-dependent way after morphine administration in different doses. Gel electrophoresis in sodium-dodecyl sulphate (SDS) revealed that proteins with subunits in molecular weight (m.w.) regions of 40,000-50,000 were increased in the cells and in the medium after morphine (10(-6) or 10(-5) M) treatment. Labeled proteins with subunits of approximately 40,000 and approximately 70,000 in the cells and medium, respectively, were decreased after morphine treatment. By exploring the field of glial protein metabolism after morphine treatment, information could be obtained on the interactions between astroglia and neurons in opiate action and during tolerance development.

Animals↗
PubMed1983

Dose and time dependent effects of morphine on the incorporation of [3H]valine into soluble brain and liver proteins.

Morphine (10(-6)-10(-5) M) causes an increase in incorporation of [3H]valine into soluble proteins during 4 hr in rat brain cortical slices, liver slices and cultivated astroglial cells. The effects are dose-dependent. They are neither cell specific nor strictly related to classical opiate receptors. Pulse-labeling with [3H]valine for 60 min after incubation in 10(-6)-10(-5) M morphine, resolves time-dependent changes in incorporation, with both increases and decreases in protein metabolism.

Animals↗
PubMed1983

Protein metabolism in an astroglial primary culture.

Protein metabolism was studied in astroglial primary cultures, grown for different time periods. Removal of fetal calf-serum for two days led to a morphological differentiation consisting of retraction of cell soma and extention of processes. There was a prominent decrease in total soluble protein and a decrease in [3H]valine incorporation into soluble protein. Dibutyrylcyclic-3'-5'-adenosine monophosphate (dB-cAMP)-treatment for two days also changed morphology in a similar way, but had no effect on [3H]valine incorporation into protein. After addition of soluble brain extract to the cultures an increased [3H]valine incorporation into soluble protein was seen together with a morphological differentiation, more pronounced in the presence than in the absence of fetal calf-serum. Proteins were secreted from the cells into the incubation medium and studied by electrophoresis. The more prominent protein bands had m.w. in the region of 10,000-100,000 daltons. The amount of newly synthesized proteins released into the medium was unchanged (or decreased slightly in 14 and 16 day old cultures) after addition of dB-cAMP" or soluble brain extract, and was much reduced after removal of fetal calf-serum.

Animals↗
PubMed1983

MAO COMT, and GABA-T activities in primary astroglial cultures.

Cultures from cerebral hemispheres of newborn rats contain the enzymes monoamine oxidase (MAO), catechol-O-methyltransferase (COMT), and gamma-aminobutyric acid alpha-ketoglutarate transaminase (GABA-T). The COMT activity was higher in the cultures than in adult rat cerebral hemispheres. The MAO activity was comparable in the cultures and in the rat cerebral hemispheres. The activities of both these enzymes increased with age in the cultures and in the rat brain hemispheres. In the culture the activities were further potentiated by removal of fetal calf serum and addition of 0.1 mM dibutyryl-cyclic AMP (dB-cAMP). GABA-T activity was, however, lower in the cultures than in the adult rat brain hemispheres. The activity increased in brain during postnatal maturation. No changes in the enzyme activity were observed in the cultures, either during growth or after removal of fetal calf serum and addition of dB-cAMP.

4-Aminobutyrate Transaminase↗
PubMed1982

Brain primary culture-- a characterization (part II).

Brain primary cultures from newborn rat hemispheres have been further characterized. THe cultures contained the glial fibrillary acidic protein (GFAp; alpha-albumin), as analyzed quantitatively. GFAp (alpha-albumin) increased in concentration during growth of cultures and during maturation of the intact rat brain. FITC-labelled antibodies against GFAp (alpha-albumin) reacted specifically with astrocyte-like cells. Theses cells were also positive after Rio-Hortega's silver carbonate staining for astrocytes. No myelin basic protein (MBP) was detected in cultures, neither by quantitative measurements nor by immunofluorescent histochemistry. Negative results were also obtained for the myelin specific enzyme 2', 3'-cyclic nucleotide 3'-phosphohydrolase (CNP), e. e. the enzyme was at the same level in the cultures of different ages as in 3-day-old rat hemispheres. However, in rat brain increasing concentrations of MBP and CNP were found during maturation. This means that no myelin and thereby probably no or few differentiated oligodendroglial cells were present in the cultures, where the predominate cell type was of astroglial nature.

2',3'-Cyclic-Nucleotide Phosphodiesterases↗
PubMed1981

Acidic protein in macrophages.

An antiserum to unstimulated rat peritoneal macrophages was produced in rabbits. The antibodies were directed against an acidic protein with a molecular weight of 35,000 and with an isoelectric point at 4.6. The macrophage acidic protein (MAP) was purified by gel filtration of rat lung soluble proteins, followed by preparative isoelectric focusing. The preparation of MAP was pure as assayed by agar gel electrophoresis and showed one precipitation peak in crossed immunoelectrophoresis against the crude antiserum directed against peritoneal macrophages. The purified MAP was used for immunization of rabbits, and the antiserum obtained was monospecific, assayed by crossed immunoelectrophoresis and Grabar-Williams immunoelectrophoresis. The titre was 4 times higher in the anti-MAP antiserum (1:80) than in the crude antimacrophage antiserum (1:20), tested against MAP by counter-current immunoelectrophoresis. The antigen (MAP) was demonstrated by direct and indirect immunofluorescence microscopy in rat blood monocytes, in spleen and lung monocytic cells, in clusters of cells in the thymus, and in adventitial macrophages around larger blood vessels in liver, kidney, lung and brain. Scattered meningeal macrophages showed fluorescence in the normal, brain. In stab-wounded areas of rat brain MAP was localized to perivascular and perineuronal macrophages with a morphology similar to that of microglial cells. The localization of the fluorescence was the same both for the antiserum against MAP and for the antiserum raised against crude peritoneal macrophages.

Animals↗
PubMed1981

A comparison of the polypeptide pattern between a brain primary culture and fractions of bulk-isolated glial cells.

This paper reports on the electrophoretic protein/polypeptide pattern of a rat brain primary culture. For comparison, the polypeptide pattern of neuronal and glial enriched fractions from adult rat brain and cerebral hemispheres from newborn and adult rat have been analysed. Water-soluble and SDS-extractable polypeptide fractions appeared and/or increased in amount in the cultures until confluency. The polypeptide pattern of the cultures most resembled that of the glial cell fractions, showing some of this fraction's specificity. Removal of fetal calf serum and addition of 0.1 mM-dibutyryl cyclic adenosine monophosphate (dB-cAMP) produced few changes in the electrophoretic pattern. The study thus provides evidence in favour of the astroglial nature of the brain primary culture. It also shows that the cells undergo some maturation in the culture.

Animals↗
PubMed1980

Brain primary culture - a characterization.

Primary cultures from rat or mouse brain hemispheres contain predominantly glial cells. These cells accumulated [3H]GABA and showed a specific fluorescence with FITC-labeled anti-S-100 antiserum. Round or elongated cells which reacted positively with antimacrophage antiserum and exhibited phagocytotic activity, were considered mesodermal macrophages. Big flat cells, unreactive to S-100 antiserum or to any other antiserum tested, showed a morphology similar to mesenchymal cells of a low differentiation grade. These cells formed a monolayer upon which other cell types grew. Positive reaction for alkaline phosphatases was used as a criterion to identify endothelial cells, the number of which increased with increasing age of the cultures. Anti-14-3-2 antiserum gave no specific reaction, indicating the presence of very few or no differentiated neurons in the cultures.

Alkaline Phosphatase↗
PubMed1980

A study of irrigation fluids for neurosurgery on brain primary cell cultures.

Primary cell cultures from newborn rat brain hemispheres were exposed to different irrigation fluids used in neurosurgery. The cells died after incubation for 5 min with hydrogen peroxide, and the number of cells was drastically decreased after 10 sec of incubation. They shrank after incubation in Elliott's artificial cerebrospinal fluid for 3 h, but the viability as determined by trypan blue exclusion test was not affected. Physiological sodium chloride, Ringer's solution and the culture medium 199 with Hank's salt had no noticeable effect on the viability or morphology of the cells.

Animals↗
PubMed1980

Pregnancy outcome for women working in laboratories in some of the pharmaceutical industries in Sweden.

The pregnancy outcome of laboratory employees in three pharmaceutical in Sweden studied with interviews, postal questionnaires, and register studies. The total material shows only a slightly increased miscarriage rate and normal perinatal mortality and malformation rates. Subdivision into two types of laboratory work, "chemical" and "other," revealed a significant difference, with an increased perinatal death rate and an increased rate of major malformations in the chemical group. This observation supports earlier findings indicating that work during pregnancy in a chemical laboratory can represent a hazard to the fetus.

Abnormalities, Drug-Induced↗
PubMed1980

Budesonide: a new corticosteroid in bronchial asthma.

The time course of response to budesonide in a dose of 1000 microgram administered by inhalation, 800 microgram given by the oral route, and 40 microgram prednisolone administered orally, has been investigated in 12 patients suffering from chronic bronchial asthma. Budesonide is an epimeric mixture of a non-halogenated glucocorticoid, 16 alpha, 17 alpha-(22R,S)-propylmethylenedioxypregna-1,4-diene-11 beta, 21-diol-3,20-dione. Inhaled budesonide and prednisolone produced a statistically significant increase in PEF 2 h after being administered. The peak effect appeared to occur between 6 and 7 h after budesonide inhalation and about 9 h following prednisolone. When given orally, budesonide failed to produce any substantial changes in PEF.

Administration, Oral↗