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Biomedical subjects

E Haneke

Publications and source records attributed to E Haneke.

At least 73 records · Page 4Linked to original sources
PubMed1987

Skin tumor of T accessory cells (interdigitating reticulum cells) with high content of T lymphocytes.

A case of T-accessory cell tumor of the skin in a 67-year-old man is reported. The limbs, shoulders, and face were affected, but no visceral involvement is evident 6 years after onset. Tumor cells are nonphagocytic mononuclear cells with folded irregular nuclei. Immunologically, cells were positive for S100 protein, HLA-DR, Ki-M1, Leu 3a (CD4), Leu 6 (CD1); that is, they are identical to the phenotype of Langerhans or interdigitating reticulum cells (IDCs). Birbeck granules were absent. The clinical course appears to be less aggressive than that of the reported IDC sarcomas in other anatomical sites. The similarity of our case to some cases of so-called "non-X histiocytosis" of the skin is discussed. It is suspected that the "non-X histiocytosis of the skin" reported in the literature might have included T-accessory cell tumors, especially those of IDC origin. More immunological studies on the histiocytic disorders of the skin are necessary to clarify their cytogenesis.

Aged↗
PubMed1987

Peanut agglutinin immunohistochemistry of basal cell carcinoma.

103 biopsies of basal cell carcinomas (BCCs) were studied using peanut agglutinin (PNA), PNA antibody and the peroxidase-antiperoxidase technique; 53 specimens of various skin tumors were examined as controls; 96% of the BCCs showed a band-like peritumorous reaction not seen in any other tumor except for the Pinkus' fibroepithelioma. The peritumorous PNA-binding was continuous in 51% of the BCCs studied and discontinuous in 45%; only 4% were completely negative. Both fibroepitheliomas revealed a discontinuous PNA-positive band. A narrow basement membrane-like positivity was seen around some small hair follicles situated within 2 neurofibromas and under one seborrhoeic keratosis. The PNA-binding band is apparently neither fibronectin, laminin, Type IV or Type V collagen and is not a constituent of normal epidermal, adnexal and vascular basement membranes.

Basement Membrane↗
PubMed1987

[Retention of ketoconazole in the skin following oral treatment].

Up to now there have been no data on how long ketoconazole is retained in the skin after administration has been discontinued. In our study, 17 probands received 200 mg ketoconazole daily for 10 days. Shave biopsies were taken from the buttocks on the last treatment day as well as 5 and 10 days later. Ketoconazole concentrations were determined by high-pressure liquid chromatography after extensive homogenization of the skin specimens. At the end of the treatment period, about 2 h after intake of the last tablet a median ketoconazole skin concentration of 3.6 micrograms/g was determined. Five days after the end of treatment, the median ketoconazole skin level was 2.5 micrograms/g and after 10 days it was still 1.2 micrograms/g. Using a bioassay with Candida albicans strain ATCC 28,516, no demonstrable amount of ketoconazole could be seen in the hair roots and adjacent hair shafts at days 10, 15 and 20 of the trial. These results suggest that ketoconazole reaches the horny layer by passive diffusion and that it is strongly adsorbed by keratin. Clinical experience indicates that repeated short-term treatment with ketoconazole provides an effective prophylaxis against the recurrence of superficial mycosis; our results represent experimental confirmation of this experience.

Administration, Oral↗
PubMed1987

[Clinical aspects and therapy of frequent diseases of the oral mucosa].

Different diseases of the oral mucosa may closely resemble each other and may therefore pose considerable differential diagnostic problems. The most frequent oral mucous membrane disorders are briefly presented according to their main clinical feature. Recurrent aphthous ulcerations have to be differentiated from viral infections causing aphthoid defects. Blisters and erosions are characteristic for erythema multiforme and oral lesions of bullous autoimmune dermatoses. Leucoplakias may be due to exogenous irritant factors, but may also be a symptom of oral disease. Warts and granulomas may simulate malignant tumours. Pigmentations are due to a variety of endogenous and exogenous factors. Some very frequent, but harmless alterations of the tongue are also briefly described.

Diagnosis, Differential↗
PubMed1986

[Fulminant acne: therapy with 13-cis-retinoic acid and indomethacin].

Young men with mild acne vulgaris may rarely experience a very acute exacerbation with ulceration and necrosis of the lesions accompanied by fever, malaise, loss of weight, often by polyarthritis, and occasionally by erythema nodosum. Elevated sedimentation rate and marked leucocytosis are characteristic; microhematuria is often observed. This condition has been called acne fulminans. We report on a young male patient suffering from severe acne fulminans. Systemic 13-cis retinoic acid (Roaccutan, Accutane) and indomethacine (Amuno) treatment rapidly improved the skin and joint lesions. Intracutaneous tests with viral, bacterial, and fungal antigens before and during treatment suggest a temporary cellular immune defect.

Acne Vulgaris↗
PubMed1986

[Immunohistochemical detection of carcinoembryonic antigen and protein S-100 in sweat gland tumors].

Carcinoembryonic antigen (CEA) and S 100 protein (S 100) have been demonstrated in formalin-fixed paraffin sections of eccrine and apocrine sweat glands, and tumors of the sweat glands, as well as, for comparison, in a variety of tumors deriving from epidermal and follicular structures using the peroxidase-antiperoxidase technique. CEA is detectable, as a membrane antigen, in the normal coil of eccrine sweat glands and in the cytoplasm of luminal cells in eccrine and apocrine sweat ducts up to the horny layer. Nearly all tumors of the sweat glands contain variable amounts of CEA. S 100 is localized in the cytoplasm of eccrine coils and secreted with the sweat. Apparently it is only found in tumors derived from the eccrine and apocrine sweat gland coils. Immunohistochemical demonstration of CEA and S 100 thus facilitates the diagnosis of tumors of the sweat glands and their differentiation from epidermal and follicular tumors.

Apocrine Glands↗
PubMed1986

[Tricholemmal carcinoma. Report of 11 cases].

Eleven cases of tricholemmal carcinoma are reported. The histological features of tricholemmal carcinoma are compared with those of tricholemmoma. The tumor is very rare and occurs in slightly damaged skin of elderly people. Whereas its clinical appearance is nonspecific, it can be differentiated from tricholemmoma by the occurrence of marked cellular and nuclear atypia and less orderly architecture. Although the histological picture suggests a high-grade malignancy, its biological behaviour appears to be relatively benign. To date, no metastases have been observed in our patients.

Aged↗
PubMed1985

Electron microscopy of Merkel cell carcinoma from formalin-fixed tissue.

Merkel cell carcinomas have characteristic, but not pathognomonic, histomorphologic features. The diagnosis can be confirmed by immunohistochemistry studies and electron microscopy. However, differential diagnostic problems often occur only after all tumor material has been fixed in formalin and embedded in paraffin, which gives poor tissue preservation for electron microscopy. Therefore, in addition to histologic and immunohistochemical studies, parallel ultrastructural investigations were performed on tumor specimens fixed in glutaraldehyde and routinely processed, for electron microscopy, on formalin-fixed tissue and on formalin-fixed paraffin blocks. Formalin fixation led to an almost complete loss of neurosecretory granules and cell membranes; however, the characteristic paranuclear whorls of intermediate filaments were retained in all tumors. Merkel cell carcinomas are immunoreactive for neuron-specific enolase (NSE), but NSE-antisera have only recently become commercially available. Cytokeratin is demonstrable as a paranuclear clump, and this feature was found in four out of six tumors. Thus histologic, immunohistochemical, and electron microscopy studies of formalin-fixed tissue are able to confirm the diagnosis of neuroendocrine Merkel cell carcinoma.

Adenocarcinoma↗
PubMed1985

Electron microscopic demonstration of virus particles in hand, foot and mouth disease.

Early skin and oral mucous membrane lesions of hand, foot and mouth disease (HFMD) were biopsied and investigated by light and electron microscopy. Histology showed spongiosis, intraepidermal splits indicating beginning vesicle formation, exocytosis of lymphoid cells and macrophages, and individual necrotic keratinocytes. Electron microscopy revealed naked viruses with a mean diameter of 24 nm which were arranged in sheets in the cytoplasm. Many keratinocyte nuclei contained tonofibrils. Cellular injury ranged from slight cytoplasmic edema to keratinocyte necrosis. Some nuclei showed dissolution of the nuclear membrane and dislocation of cytoplasm into the nucleus.

Biopsy↗
PubMed1985

Clonal heterogeneity in a case of Merkel cell carcinoma demonstrated by flow cytometry.

A 66-year-old female patient is presented in whom a rapidly growing tumour developed on the glabella. Light and electron microscopy confirmed Merkel cell carcinoma. Flow cytometry (FCM), performed of a tumour specimen, yielded multiple aneuploid stem lines. The FCM data are discussed with regard to the histological features and the biological behaviour of the tumour.

Aged↗
PubMed1985

Thymopentin treatment of herpes simplex infections. An open, monitored, multicenter study.

Twenty-seven patients suffering from long-standing, severe, recurrent herpes simplex (14 labial and 13 genital) who had not responded adequately to prior therapy were recruited for this open, monitored study. They were treated with thymopentin 50 mg subcutaneously three times weekly for a period of 6 weeks. Clinical controls were performed once a week and then again 6 weeks after cessation of therapy; laboratory investigations were done at time points 0, 3, and 6 weeks. Additionally, information was collected with regard to the clinical course during the following year. Thirteen of 14 patients with labial infection and 10 of the 13 with genital herpes improved markedly (p less than 0.05) as shown by decrease in the relapse rate of at least 50%, shorter relapse episodes, and improvement of symptoms such as pain and itching. Fourteen of these 27 patients experienced no relapse for a period longer than 4 months after cessation of therapy. These favorable results were paralleled by a statistically significant increase in the T cell helper/suppressor ratio. This finding indicates that thymopentin acts as an immunodomulator; it is assumed that the activation of T helper cells induces-presumable via interleukin 2-the proliferation of cytotoxic T lymphocytes and natural killer cells which play a major role in the natural immune defense. No serious side effects of thymopentin were recorded.

Adjuvants, Immunologic↗