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E H Coe

Publications and source records attributed to E H Coe.

25 records · Page 2Linked to original sources

Control of anthocyanin synthesis by the C locus in maize.

Alleles at the C locus in maize include C, one of the complementary genes required for anthocyanin pigmentation in the aleurone tissue of the mature kernel; C-I, dominant inhibitor of pigmentation; and c, recessive colorless. The recessive colorless alleles can be differentiated into two distinct forms: c-p (p for positive), conditional colored, develops pigment in the light during germination; c-n (n for negative), colorless, fails to develop pigment at any time. Four-point linkage data support the differentiation of c-p (in the alleles derived from either W22 or K55 inbreds) from c-n. Light and germination conditions are both required for anthocyanin synthesis in c-p tissue, but light "induction" and germination "induction" are two separable events inasmuch as the light stimulus can be stored. The ratio between the two major pigments, cyanidin and pelargonidin, is lowered in c-p tissue relative to that in dominant colored (C) tissue for the W22 allele and background but not for the K55 allele and background. Segregation tests reveal that the difference in the cyanidin-to-pelargonidin ratio in the C and the c-p tissue of W22 background is associated with the C locus. Several properties of the C locus, including anomalous allelic functions, stage- and properties of the C locus, including anomalous allelic functions, stage- and condition-dependent anthocyanin synthesis controlled by its multiple allelic series, and tissue-specific function, suggest that C may be a regulatory locus in the control of anthocyanin synthesis in aleurone tissue.

Alleles↗

Gene-dependent flavonoid glucosyltransferase in maize.

A direct relationship between a specific gene and a specific enzyme involved in flavonoid biosynthesis is reported for the gene Bz and uridine diphosphoglucose: quercetin glucosyltransferase in maize pollin, seedlings, and seeds. Ratios are presented for specific activities of the glucosyltransferase from pollen, seed, and seedling tissues homozygous and heterozygous for Bz and homozygous for bz.

Flavonoids↗

Salmon silk genes contribute to the elucidation of the flavone pathway in maize (Zea mays L.).

We utilized maize (Zea mays L.) lines expressing the salmon silk (sm) phenotype, quantitative trait locus analysis, and analytical chemistry of flavone compounds to establish the order of undefined steps in the synthesis of the flavone maysin in maize silks. In addition to the previously described sm1 gene, we identified a second sm locus, which we designate sm2, located on the long arm of maize chromosome 2. Our data indicate that the sm1 gene encodes or controls a glucose modification enzyme and sm2 encodes or controls a rhamnosyl transferase. The order of intermediates in the late steps of maysin synthesis was established as luteolin --> isoorientin --> rhamnosylisoorientin --> maysin.

Chromatography, High Pressure Liquid↗