Search PubMed⌕ Search

Biomedical subjects

E Gruber

Publications and source records attributed to E Gruber.

At least 55 records · Page 3Linked to original sources
PubMed1981

The effect of chondroitin-sulfate and hyaluronic acid on glycosaminoglycan and collagen synthesis and secretion by cultured arterial endothelial cells.

The effect of the addition of chondroitin-sulfate or hyaluronic acid to the culture medium on the glycosaminoglycan and collagen content and on the glycosaminoglycan composition of pig arterial endothelial cells and of the culture medium has been studied by biochemical and morphological methods. Both chondroitin-sulfate and hyaluronic acid were found to be transported to the interior of the cultured cells and then degraded. CSA, in addition enhanced collagen synthesis within the cells, but not the rate of excretion. Hyaluronic acid did not influence collagen formation.

Animals↗
PubMed1981

Age-related changes in the glycosaminoglycans and collagen of cultured pig endothelial cells.

Pig aortic endothelial cells were grown from aortic intimal explants. Tissue culture samples were obtained at regular intervals between the 2nd and 21st day (time-course experiments) and between the 2nd and 15th passage (influence of subcultivation). Glycosaminoglycans (GAG-s) were isolated separately from the cells and from the medium. The various GAG-s were identified by electrophoresis on cellulose acetate strips, by differential degradations with HNO2 and by digestion with selective enzymes, and their concentration was measured. Protein and collagen content was also determined. Parallel tissue culture monolayers were investigated by microscopical methods. Total GAG and collagen concentrations showed maximum curves when plotted as a function of the time of incubation, with peak values on the 9th day, both in the cells and in the medium. From among the individual GAG-s the amount of hyaluronic acid and chondroitin-sulfate increased gradually, while heparan-sulfate content changed in the opposite direction. Chondroitin was detectable only in the culture medium; its concentration decreased steeply until the tenth day. Alterations in the dermatansulfate content showed inverse tendency. The production and excretion of collagen and of GAG-s increased during the first six passages, both in the cells and in the media, and then declined during the four following ones. The ratio of the individual GAG-s did not alter until the tenth subculture. Further subcultivation resulted in a gradual increase in the relative amount of chondroitin-sulfate and a decrease in heparan sulfate contents.

Aging↗
PubMed1980

Nature of permeability changes in membrane of HeLa cells adsorbing Sendai virus.

Adsorption of Sendai virus to HeLa cells induced in them an increased permeability to K+, Na+, Ca++, deoxyglucose, but not to fluorescein. The stimulation of uptake of 42K was temperature-dependent, did not occur below 15 degrees C, and was not inhibited by ouabain. The virus-induced increase in the uptake and release of 42K and of 3H deoxyglucose could not be mimicked by treatment of cells with linoleic acid, a procedure which increased the fluidity of the cellular membranes. The stimulatory effect of 0.5 mM ATP on the release of deoxyglucose was enhanced several fold in the presence of Sendai virus. These results seem to indicate the possible involvement of membranal enzymes such as e.g. protein kinase in the permeability changes induced by Sendai virus.

Calcium↗
PubMed1980

Contact lens versus intraocular lens in the correction of aphakia.

The insertion of an intraocular lens at the time of cataract surgery has become a very popular method of correcting aphakia. However, it is not done without adding extra risks to the operation and postoperative course. The use of a contact lens, though not quite as effective from a visual standpoint, involves fewer risks. The use of the extended-ear soft contact lens will further enable to correction of aphakia. The more the extended-wear lens becomes successful, the less need will there be for an intraocular lens.

Aphakia, Postcataract↗
PubMed1979

Complications associated with spin-cast soft contact lenses.

The only spin-cast soft contact lens manufactured in the United States is the Bausch & Lomb Soflens. There have been no significant complications reported with this high-quality lens. Essentially, the complications seen with the spin-cast lens have been the same as those seen with any of the lathe-cut hydrophilic lenses. The two most common complications have been poor centering of the lenses and deposits on the lenses.

Contact Lenses, Hydrophilic↗
PubMed1977

Carbon-14 tracer studies in rat-liver perfusion experiments under conditions of gluconeogenesis from lactate and pyruvate.

The intracellular events in the metabolic pathway of gluconeogenesis from lactate and pyruvate in liver tissue were assumed to be understood. Nevertheless the results of several 14C-tracer experiments gave rise to the postulation of still unknown intracellular interactions under this condition. We have tried to contribute to the solution of this problem by using different 14C labeled tracers such as [1-14C]lactate or pyruvate and [2-14C]lactate or pyruvate, [14C]bicarbonate and [1-14C]octanoate in perfusion experiments with livers from rats under conditions of gluconeogenesis from lactate and pyruvate. The 14C labeling patterns of intracellular metabolites as malate, citrate, phosphoenolpyruvate, phosphoglycerate and newly synthesized glucose were analysed under different conditions. A comparison with values calculated by using metabolic models, based on the generally accepted concepts of intracellular interactions, showed some fundamental discrepancies, which justify the postulation of still unknown intracellular interactions in the pathway of gluconeogenesis from lactate and pyruvate.

Acetoacetates↗
PubMed1977

Carbon-14 tracer studies in the metabolism of isolated rat-liver parenchymal cells under conditions of gluconeogenesis from lactate and pyruvate.

In rat liver perfusion experiments under conditions of gluconeogenesis from lactate and pyruvate 14C-labeling patterns of metabolites with (1-14C)-labeled and (2-14C)-labeled lactate or pyruvate, [14C]bicarbonate and [1-14C]octanoate as tracers, have been obtained, which do not agree with generally assumed reaction schemes. The experiments have been repeated with incubations of isolated rat-liver parenchymal cells. The results demonstrate that the discrepancies between expected and analysed 14C-labeling patterns of metabolites were still existent. From this observation it may be concluded, that 14C-labeling patterns of metabolites are indicative for the existence of still unknown metabolic relationships in liver parenchymal cells. Furthermore the results of our experiments prove that conclusions based on the exclusive analysis of metabolite levels are of limited value for studying intracellular events, because of uncharacterized compartmentations, which become evident in 14C-tracer studies. It cannot be answered by our studies, whether the apparent existence of differently labeled species of citrate, oxoglutarate or acetyl-CoA, represent intracellular compartmentation or whether it is the result of metabolic heterogeneity of liver parenchymal cells.

Acetates↗