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Biomedical subjects

E Greene

Publications and source records attributed to E Greene.

At least 19 recordsLinked to original sources
PubMed1998

Red squirrels, Tamiasciurus hudsonicus, produce predator-class specific alarm calls

Red squirrels, can produce alarm calls when they detect a potential predator. Observations of natural interactions between red squirrels and large birds, and predator-presentation experiments in the field, showed that red squirrels produce acoustically different alarm calls in response to aerial danger (live birds and a model hawk flown towards them) versus danger approaching from the ground (dogs and humans). The alarm call produced in response to aerial danger is acoustically convergent on the 'seet' alarm call produced by many species of passerine birds in response to raptors. The squirrels' 'seet' alarm is a short, low-amplitude, high-frequency call. These characteristics make the call difficult to localize, and is in a frequency range that is poorly perceived by raptors. Red squirrels produce much louder, wide-bandwidth bark calls in response to terrestrial danger. This is the first demonstration of predator-class specific alarm calls of red squirrels. Copyright 1998 The Association for the Study of Animal Behaviour Copyright 1998 The Association for the Study of Animal Behaviour.

Journal Article↗
PubMed1998

A test of the gravity lens theory.

Naito and Cole [1994, in Contributions to Mathematical Psychology: Psychometrics and Methodology Eds G H Fischer and D Laming (New York: Springer)] provide a configuration which they describe as the Gravity Lens illusion. In this configuration, four small dots are presented in proximity to four large disks, and one is asked to compare the slope of an imaginary line which connects one pair of dots with the slope of a line which connects the other pair. In fact the slopes are the same, i.e. their axes are parallel, but because of the positioning of the large disks they appear to be at different orientations. Naito and Cole propose that the perceptual bias is analogous to the effects of gravity on the metrics of physical space, such that mental projections in the vicinity of a disk (or an open circle) are distorted just as the path of light is bent as it passes a massive body such as a star. Here we provide a simple test of this concept by having subjects judge alignments of dots which lie near tangents to a circle. Subjects were asked to project straight lines through pairs of stimulus dots, selecting and marking points in open space which were collinear with each pair. As would be predicted by the Gravity Lens theory, the locations selected by subjects were displaced from straight lines. However, the error magnitudes were substantially larger for judgments of dot pairs which had an oblique alignment, as compared with dot pairs which were aligned with a cardinal axis. This differential of effect as a function of stimulus orientation is not predicted by the gravity concept.

Form Perception↗
PubMed1998

Similar Müller-Lyer effects from operant and comparison response modes.

A one-sided (monopole) version of the Müller-Lyer was used to assess the effect of response mode on the metric distortion associated with the illusion. Two different response modes were tested for comparability. The Operant Mode required the judgment of stimulus span to be indicated by marking a dot on the test page. The Comparison Mode required judgment of equality of two adjoining prepositioned spans. The perceptual effects with the comparison procedure are known to consist of underestimation of spans which are bounded by concave fin-sets and over-estimation of spans which are bounded by convex fin-sets. The question is whether the motor demands of the operant task produce an additional source of metric bias. Analysis indicate a very high correlation of judgments for the two modes of testing. Also, data with either mode of responding closely fit a linear model of the effect, and the model provides comparable index values for the concave and convex versions. These results should mitigate concern about potential motor bias from the operant method and encourage its use as a more efficient procedure for assessing metric distortion.

Distance Perception↗
PubMed1997

Evaluating the decay gradient for collinearity bias with lateral displacement from the axis of induction.

The misperception of alignment which is found in many geometric illusions can be quantified using relatively simple stimulus configurations. Perceived collinearity of one segment (designated as the test segment) is biased by a second segment (designated as the induction segment), with the size of effect being a function of the relative angle between the two segments. The process can be described as angular induction. The strength of bias is greatest when the induction segment is centered at the tip of the test segment. Tong and Weintraub have reported that lateral displacement from the tip, i.e., at right angles to the axis of the induction segment, produces a sharp drop in the strength of effect. This decline is described as a "decay gradient" for the angular induction. One experiment replicates and provides better quantification of this "decay gradient". Two other experiments examine the decay gradient using a pair of induction segments, one on each side of the tip of the test segment. Displacement of the segments (either in the same direction or in opposite directions) produces substantially the same gradient of effect. Therefore, previous evidence of "tandem boosting" of effect for segment pairs does not depend on collinearity among the stimulus components. Finally, a fourth experiment finds that an induction segment which is at a fixed position and orientation differentially affects the influence of a variable induction segment. At some angles the influence of the variable segment is augmented, and at others it is suppressed. These findings are discussed in a neuroreductionist context, and a simple model for angular induction is presented.

Analysis of Variance↗
PubMed1997

Evaluating Müller-Lyer effects using single fin-set configurations.

Four experiments were conducted to study the bias of perceived length for Müller-Lyer configurations that contained a single set of fins (i.e., two segments that join to form a vertex). The experiments manipulated several factors that have been shown to be critical to the effect: (1) version (which way the apex pointed), (2) length of the stimulus span, (3) presence or absence of a line segment in the span being judged, (4) fin length, (5) fin angle, and (6) the zone in which the response was rendered. Using percent error as the index of perceptual distortion, the major finding was that the two versions show an opposite slope for strength of effect as a function of span. When stimulus spans were plotted against response means (not converting to percent error), an almost perfect linear relation was found. These results indicate that the perceptual effects can be modeled as a linear system having two parameters through which the treatments exert their influence. The results are discussed in relation to major theories of mechanism for the Müller-Lyer illusion.

Adult↗
PubMed1996

Synaptic membrane G proteins are complexed with tubulin in situ.

The G proteins G S and Gi1 appear to be capable of binding to tubulin specifically, and it has been suggested that such binding results in G protein activation via direct transfer of GTP. This study was undertaken to demonstrate that consequences of G protein activation by tubulin, i.e., stimulation or inhibition of adenyl cyclase, were dependent on the G proteins expressed as well as unique aspects of the membrane or cytoskeleton in a given cell type. Membranes from rat C6 glioma cells, which express G s alpha but not G i alpha 1, responded to the addition of tubulin with a stable activation of adenyl cyclase. Conversely, membranes from rat cerebral cortex, which contain both G s and G i 1, responded to exogenous tubulin with a stable inhibition of adenyl cyclase. Unlike C6 membranes, cerebral cortex membranes are richly endowed with tubulin, and antitubulin antibodies immunoprecipitated complexes of tubulin and G i 1 and G s from detergent extracts of these membranes. Nearly 90% of the G s alpha from Triton X-114 extracts coimmunoprecipitated with tubulin, suggesting that these proteins exist as a complex in the synaptic membrane. Such complexes may provide the framework for a G protein-cytoskeleton link that participates in the modulation of cellular signal transduction.

Adenylyl Cyclase Inhibitors↗
PubMed1995

Probing collinearity bias in the close induction field.

The substrates of the Poggendorff illusion can be evaluated using as few as two line segments, a test segment whose collinearity is judged, and an induction segment which serves to bias that judgment. Previous research from this laboratory has shown that a very short induction segment can produce a substantial bias of perceived collinearity when it is centered at the tip of the test segment, and there is some evidence that the symmetry of this configuration affects the strength of this bias. Four experiments were conducted to clarify the issue of symmetry in the stimulation of the "half-field" zones which lie on each side of the tip. That Exp. 1a showed displacements which moved the induction segment to an eccentric position, i.e., occupying a single half-field, reduced collinearity error. With additional displacement which produced a gap there was a rebound of induction effect. Exp. 1b indicated that an eccentric induction segment does not produce the usual strength or pattern of bias as a function of its orientation relative to the test segment. Exp. 2a suggested that the strength of induction effect is a function of symmetry within the half-fields rather than the lengths of the segments per se. Finally, in Exp. 2b the strength of induction was a joint function of the position of a short segment within one half-field and the length of another segment which stimulated the other half-field. The effect showed a complex oscillation with changes in position. We discuss the induction mechanism as beginning with "contour filters" which register the induction segment(s) and which combine their responses on the basis of "tandem activation" of the close half-fields.

Form Perception↗
PubMed1994

Anomalous and luminance contours produce similar angular induction effects.

One can shear a pattern of lines to produce an anomalous contour which has a perceptual influence similar to that of a straight line segment. Illusion effects have been found with configurations which contain these anomalous contours, as well as cross adaptation with respect to luminance contours. We have found that sheared-line contours will bias judgment of collinearity, ie perceived alignment, of a luminance contour. The angular induction effects are similar to those reported for interactions between luminance contours, and the same equation can be used to model both kinds of data. The results of this experiment support the neuroreductionist view that anomalous and luminance contours are processed at the same level of the nervous system. Additionally, we suggest that with both types of contour the perceptual system registers and responds to the alignment of local brightness differentials.

Adult↗
PubMed1994

Angular induction as a function of the length and position of segments and gaps.

The perceptual distortions which are manifested in the Poggendorff illusion can be studied with the use of a more restricted set of stimulus elements. Experiments were designed in which angular induction effects between two line elements, known respectively as the test segment and induction segment, were evaluated. In some stimulus configurations the induction 'segment' consisted of a tandem pair of segments. Previous studies had shown that the induction segment will bias operant judgments of collinearity for a test segment, this effect being a function of the relative angle between the two. Six experiments are reported, in which the length and position of segments in relation to the tip of the test segment were varied. It was found that substantial induction is produced by a very short segment, and that this can bias judgment even when its displacement spans more than 10 deg of visual angle. Several aspects of the data suggest that the strength of effect is a log-linear function of segment position. However, the results from displacement of single or tandem segments do not conform to predictions based on length/response summation, and thus do not support a linear-systems approach. Neural substrates for these interactions are given brief attention.

Adult↗
PubMed1994

Collinearity judgment as a function of induction angle.

The misalignment which is seen in the Poggendorff illusion can be studied with better control by using a configuration which has only two line segments. Two experiments were conducted in which subjects judged collinearity of a test segment, this judgment being subjected to a biasing influence from a second (induction) segment. Exp. 1 held the test segment at one of three orientations relative to the observer (30 degrees, 45 degrees, and 60 degrees) and systematically varied the orientation of the induction segment in 15 degrees increments through the range of possible positions. The orientation of the page relative to the observer was varied as well. Exp. 2 varied the test segment through a greater range of angles and sampled more levels of induction segment orientation. Analysis indicated that projection errors follow orderly rules similar in kind to but different in magnitude from those observed for the Tilt Illusion, most notably, (a) misprojection is greatest when the orientation of the interfering line is similar to that of the line segment being projected and (b) the strength of this influence decreases as the relative angle becomes orthogonal. Also, the orientation of the segment being projected relative to the observer serves to modulate the strength of the basic induction effect. These perceptual interactions are discussed in relation to neural models for orientation selectivity.

Adult↗
PubMed1994

Classical geometric illusion effects with nonclassical stimuli: angular induction from decomposing lines into point arrays.

Angular induction is the process by which one line segment can bias judgment of orientation and/or collinearity of another segment, and it has been established that the magnitude of error is a determinate function of the relative angle between the two. We examined how these known relationships are affected by decomposing the induction segment into an array of scattered points. The bias that was produced by such arrays was found to be consistent with a formal model of angular induction, with the strength of the effect decreasing as the scatter among the points was increased. This decline in strength was almost linear with a logarithmic transform of the dimensions of the stimulus array. We also evaluated the hypothesis that the induction stimulus is detected by one or more channels--for example, neurons--for which the sensitivity profiles are modeled as Gabor wavelets. The change in induction strength with increasing point scatter was not predicted by a single width of channel. However, the combined activity of an ensemble of channels that differed in width did match the perceptual effects if one also stipulated that each channel would respond maximally to a fine-line stimulus.

Adult↗
PubMed1993

Protective effects of anti-O polysaccharide and anti-lipid A monoclonal antibodies on pulmonary hemodynamics.

Monoclonal antibodies (MAbs) directed to endotoxin can protect in some animal models against the pathophysiological effects of endotoxin infusion. When 0.02 microgram/kg of lipopolysaccharide (LPS) derived from Escherichia coli O111:B4 was incubated in vitro for 2 h with the murine immunoglobulin G MAb, 5B10, directed against the O-polysaccharide antigenic domain of E. coli O111:B4 and then the mixture was infused into sheep, we noted significant protection. The second temperature peak was decreased (P < 0.05 vs. LPS control). The acute pulmonary arterial pressure elevation was diminished (mean peak pulmonary arterial pressure 23.2 +/- 2.5 mmHg, P < 0.05 vs. LPS control), and the peak plasma thromboxane B2 level was reduced (mean peak thromboxane B2 level 0.50 +/- 0.15 ng/ml, P < 0.05 vs. LPS control). In contrast, preincubation of the LPS with a human immunoglobulin M MAb, HA-1A, directed against the core glycolipid of the LPS molecule provided no protective effects in this sheep model. This finding is in agreement with recent studies reporting HA-1A may bind to antibiotic-treated bacteria but not to purified smooth LPS.

Animals↗
PubMed1993

Protective effects of E5, an antiendotoxin monoclonal antibody, in the ovine pulmonary circulation.

The cross-protective effects of a murine immunoglobulin M monoclonal antilipid A antibody (E5 MAb) were tested by challenging awake sheep with mixtures of in vitro incubated E5 MAb (0.02 mg/kg) with lipopolysaccharide (LPS, 0.02 micrograms/kg) derived from Escherichia coli O111:B4, E. coli O55:B5, or Serratia marcescens. Intravenous infusion of these LPS preparations without antibody into awake sheep produced a similar pattern of fever, leukopenia, plasma thromboxane B2 (TxB2) release, and acute pulmonary vasoconstriction with pulmonary hypertension. The addition of MAb E5 to LPS from E. coli O111:B4 reduced these responses to the LPS in a fashion comparable to that achieved with an MAb specific to the E. coli O111:B4 O-side chain. Incubation of LPS derived from E. coli O55:B5 with the E5 MAb only slightly diminished acute pulmonary hypertension, the delayed temperature increase, and the degree of leukopenia (all P = NS) but reduced the mean peak TxB2 at 60 min (P < 0.05) compared with a control infusion of E. coli O55:B5 LPS. We were unable to demonstrate any protective effects on the pulmonary circulation from incubating E5 with LPS derived from S. marcescens. Preincubation of B55 MAb (a murine immunoglobulin M MAb directed against a human milk fat globulin), the control antibody, with LPS from E. coli 0111:B4 decreased the mean peak TxB2 but had no effect on the other parameters. We conclude that incubating E5 with LPS protects the pulmonary circulation of sheep from challenge with LPS derived from the parent E. coli strain. There were trends toward protection by E5 against LPS from 055:B5 E. coli, but these did not reach statistical significance.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

Both tilt and misalignment are manifestations of angular induction.

With a Poggendorff configuration one sees two obliques as being misaligned, and with a Judd configuration one sees them as having different tilts, i.e., as being misoriented. It has been suggested that these are separate illusory effects. I submit, however, that one can see misorientation in the double Poggendorff and misalignment with a Judd-like configuration. It seems likely that both perceptual effects are manifestations of the same process.

Attention↗
PubMed1993

Angular induction is modulated by the orientation of the test segment but not its length.

Angular induction is the basic process by which the orientation of line segments can affect the perceived orientation of other segments as well as their alignment. In this laboratory, we have found that the effect on alignment follows approximately linear rules, with the inducing segment having its greatest influence when its orientation is near to that of the segment being judged. Other laboratories, however, have reported peak effects when the relative angle between the two is at 45 degrees, and with the inducing segment being aligned with one of the cardinal axes of the observer. It has been said that the length of the test segment being judged is a critical factor, but the first experiment of the present study shows a similar linear decline of induction strength irrespective of test segment length. The second experiment indicates that the orientation of the test segment relative to the observer modulates the induction to determine the location of peak effects. A two-factor linear model predicts the observed pattern of results.

Adolescent↗
PubMed1993

The influence of response factors on the magnitude of angular induction.

The angular induction which is manifested in geometric illusions such as the Poggendorff can be quantified by having the subject select a point which appears to be collinear to an oblique line segment. There may be some concern, however, that this operant procedure does not control the distance which is selected by the subject and may introduce nonperceptual sources of response bias. Four experiments, involving 14 subjects, provide evidence that the error, expressed as angular departure from true collinearity, is a constant irrespective of the location of the page which is chosen. Further, this operant procedure provides a valid measure of perceptual bias.

Adolescent↗