Search PubMed⌕ Search

Biomedical subjects

E Green

Publications and source records attributed to E Green.

At least 145 records · Page 8Linked to original sources
PubMedDate not supplied

Use of reduced silver staining to show loss of connections in aged rat brain.

A variation of the Fink-Heimer reduced silver technique was used to show the extent of axonal degeneration in the brains of aged rats. Degeneration product was extensive throughout the white matter of the old brains, being most dense in the optic tract, but substantial in fornix, corpus callosum, cingulum, and anterior commissure. There was selective impregnation of hippocampal strata, suggesting that there may be differential atrophy of connections as a function of age.

Aging↗
PubMedDate not supplied

Ex vivo evaluation of organ function after cold ischemia.

An ex vivo perfusion of kidneys was performed at 34 degrees C after cold ischemia of 24, 48, 72, and 96 hours to evaluate organ function prospectively. The prospective evaluation of organ function followed static hypothermic storage of the kidneys in a solution representative of clinical organ preservation. The warm perfusion was performed with an acellular solution that supports oxidative metabolism of sufficient magnitude to restore urine flow ex vivo. The parameters of organ function evaluated included oxygen consumption, vascular resistance, urine flow, and glomerular filtration rates, which were correlated with the histologic findings. The results of this study suggest that kidneys exposed to 24 and 48 hours of cold ischemia demonstrated oxygen consumption rates and vascular dynamics similar to control kidneys without exposure to cold ischemia, indicating cell viability. When the cold ischemic period was increased beyond 48 hours of preservation, substantially reduced rates of oxygen consumption and increased vascular resistances were observed, representing a loss of viability confirmed histologically. However, organ function was found to be impaired after exposure to cold ischemia at every time point. These results suggest that cold ischemic exposure had a negative impact on immediate renal function once oxidative metabolism was restored, which was exacerbated as the cold ischemic period was extended. Furthermore, these findings suggest that although the renal cells were viable after cold ischemic exposure, the viability status did not result in immediate function. Therefore, assessment of an organ based solely on cell viability may falsely indicate a functional organ. It will be necessary to identify parameters of organ function that can distinguish reversibility from non-reversibility of cellular impairment to distinguish permanent functional disturbances. The ability to predict organ function prospectively will be an important aspect of any effective future expansion of the organ donor pool.

Animals↗
PubMedDate not supplied

Objective quantification method for measuring in vivo accumulated dental plaque.

Dental plaque was collected, dried, and weighed on a gold insert that fit into the buccal aspect of a posterior crown. The plaque weight was determined by calculating the difference between the postcollection insert weight and the precollection insert weight. This system for measurement of the dry weight of in vivo accumulated dental plaque was found to be a practical means of plaque quantification.

Adult↗
PubMedDate not supplied

Teaching a new approach to quality improvement.

Whatever the educator's role in teaching quality methods, rapid, industry-wide changes in quality practices are having a profound impact on the education department. Familiar, comfortable teaching methods for monitoring and evaluation have disappeared. Clinical practice models, long the mainstay of nursing education, have become obsolete in today's multidisciplined environment. This article describes a new approach to quality improvement that utilizes a model expanded beyond clinical practice: a tri-focus of patient, staff, and system, integrated with the four broad-based categories of standards (structure, outcome, process, and evaluation) to achieve quality improvement within the organization.

Decision Trees↗
PubMedDate not supplied

Nurse prescribers: infrastructures required to support their role.

AIM: This study aims to answer the question: what are the opinions of nurse prescribing students about infrastructures required to support nurse prescribing? METHOD: Two researchers collected data from 12 focus groups (six health visitor and six district nurse groups) made up of students undertaking a course in nurse prescribing at the University of Central Lancashire. The total number of people who participated in the study was 146. RESULTS: Ten themes were identified by the focus groups as necessary infrastructures to support nurse prescribing. CONCLUSION: The results indicate that, for nurse prescribers to fulfil their new role effectively, a variety of mechanisms are necessary. These supporting infrastructures are vital to the success of nurse prescribing and are likely to result in a more confident and competent prescribing workforce.

Attitude of Health Personnel↗